The involvement of FoxO in cell survival and chemosensitivity mediated by Mirk/Dyrk1B in ovarian cancer

  • Authors:
    • Jingchun Gao
    • Xiangjun Yang
    • Ping Yin
    • Wenfeng Hu
    • Hongfeng Liao
    • Zhihui Miao
    • Chao Pan
    • Na Li
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  • Published online on: December 12, 2011     https://doi.org/10.3892/ijo.2011.1293
  • Pages: 1203-1209
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Abstract

Minibrain-related kinase (Mirk) is a serine/threonine kinase which is also known as the dual specificity tyrosine-phosphorylation-regulated kinase 1B (Dyrk1B). It is known that Dyrk1A, the closest family member to Mirk/Dyrk1B can mediate cellular localization of mammalian forkhead subclass O (FoxO1), a transcription factor, although the effect of Mirk/Dyrk1B on FoxO factors remains to be defined. In this study, we showed that Mirk/Dyrk1B protein was overexpressed in 5 of 8 ovarian cancer cell lines and negatively correlated with the protein level of FoxO factors (FoxO1+FoxO3A). Knockdown of Mirk by small interfering RNA (siRNA) resulted in cell apoptosis and sensitized cells to cisplatin accompanied by nuclear translocation of FoxO1 and/or FoxO3A as well as increased Bim, TRADD, cleaved caspase-3 and PARP. Furthermore, combined siRNAs of Mirk with FoxO1 and/or FoxO3A led to fewer apoptotic cells and cisplatin sensitivity compared to Mirk siRNA alone, suggesting that FoxO is involved in Mirk-mediated cell survival and chemosensitivity of ovarian cancer. Taken together, Mirk/Dyrk1B plays an important role in ovarian cancer cell survival through modulating FoxO translocation and may be a novel therapeutic target for ovarian cancer.
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April 2012
Volume 40 Issue 4

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Spandidos Publications style
Gao J, Yang X, Yin P, Hu W, Liao H, Miao Z, Pan C and Li N: The involvement of FoxO in cell survival and chemosensitivity mediated by Mirk/Dyrk1B in ovarian cancer. Int J Oncol 40: 1203-1209, 2012
APA
Gao, J., Yang, X., Yin, P., Hu, W., Liao, H., Miao, Z. ... Li, N. (2012). The involvement of FoxO in cell survival and chemosensitivity mediated by Mirk/Dyrk1B in ovarian cancer. International Journal of Oncology, 40, 1203-1209. https://doi.org/10.3892/ijo.2011.1293
MLA
Gao, J., Yang, X., Yin, P., Hu, W., Liao, H., Miao, Z., Pan, C., Li, N."The involvement of FoxO in cell survival and chemosensitivity mediated by Mirk/Dyrk1B in ovarian cancer". International Journal of Oncology 40.4 (2012): 1203-1209.
Chicago
Gao, J., Yang, X., Yin, P., Hu, W., Liao, H., Miao, Z., Pan, C., Li, N."The involvement of FoxO in cell survival and chemosensitivity mediated by Mirk/Dyrk1B in ovarian cancer". International Journal of Oncology 40, no. 4 (2012): 1203-1209. https://doi.org/10.3892/ijo.2011.1293