Novel molecular targets regulated by tumor suppressors microRNA-1 and microRNA-133a in bladder cancer

  • Authors:
    • Takeshi Yamasaki
    • Hirofumi Yoshino
    • Hideki Enokida
    • Hideo Hidaka
    • Takeshi Chiyomaru
    • Nijiro Nohata
    • Takashi Kinoshita
    • Miki Fuse
    • Naohiko Seki
    • Masayuki Nakagawa
  • View Affiliations

  • Published online on: February 29, 2012     https://doi.org/10.3892/ijo.2012.1391
  • Pages: 1821-1830
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Our expression signatures of human cancer including bladder cancer (BC) revealed that the expression of microRNA-1 (miR-1) and microRNA-133a (miR-133a) is significantly reduced in cancer cells. In the human genome, miR-1 and miR-133a are located on the same chromosomal region (miR-1-2 and miR-133a-1 on 18q11.2, and miR-1-1 and miR-133a-2 on 20q13.33) called cluster. In this study, we identified the novel molecular targets commonly regulated by miR-1 and miR-133a in BC. Genome-wide molecular target search and luciferase reporter assays showed that prothymosin-α (PTMA) and purine nucleoside phosphorylase (PNP) are directly regulated by miR-1 and miR-133a. Silencing of these two genes significantly inhibited cell proliferation and invasion, and increased apoptosis in BC cells. Immunohistochemistry showed that PTMA expression levels were significantly higher in BC compared to normal bladder epitheliums. PTMA and PNP were identified as new target genes regulated by the miR-1 and miR-133a cluster in BC. These genes may function as oncogenes contributing to cell proliferation and invasion in BC. Tumor suppressive miR-1 and miR-133a-mediated novel molecular targets may provide new insights into the potential mechanisms of BC oncogenesis.

Related Articles

Journal Cover

June 2012
Volume 40 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yamasaki T, Yoshino H, Enokida H, Hidaka H, Chiyomaru T, Nohata N, Kinoshita T, Fuse M, Seki N, Nakagawa M, Nakagawa M, et al: Novel molecular targets regulated by tumor suppressors microRNA-1 and microRNA-133a in bladder cancer. Int J Oncol 40: 1821-1830, 2012
APA
Yamasaki, T., Yoshino, H., Enokida, H., Hidaka, H., Chiyomaru, T., Nohata, N. ... Nakagawa, M. (2012). Novel molecular targets regulated by tumor suppressors microRNA-1 and microRNA-133a in bladder cancer. International Journal of Oncology, 40, 1821-1830. https://doi.org/10.3892/ijo.2012.1391
MLA
Yamasaki, T., Yoshino, H., Enokida, H., Hidaka, H., Chiyomaru, T., Nohata, N., Kinoshita, T., Fuse, M., Seki, N., Nakagawa, M."Novel molecular targets regulated by tumor suppressors microRNA-1 and microRNA-133a in bladder cancer". International Journal of Oncology 40.6 (2012): 1821-1830.
Chicago
Yamasaki, T., Yoshino, H., Enokida, H., Hidaka, H., Chiyomaru, T., Nohata, N., Kinoshita, T., Fuse, M., Seki, N., Nakagawa, M."Novel molecular targets regulated by tumor suppressors microRNA-1 and microRNA-133a in bladder cancer". International Journal of Oncology 40, no. 6 (2012): 1821-1830. https://doi.org/10.3892/ijo.2012.1391