Human amniotic membrane-derived epithelial stem cells display anticancer activity in BALB/c female nude mice bearing disseminated breast cancer xenografts

  • Authors:
    • Nam-Hee Kang
    • Bo-Rim Yi
    • So Yoon Lim
    • Kyung-A Hwang
    • Young Seok Baek
    • Kyung-Sun Kang
    • Kyung-Chul Choi
  • View Affiliations

  • Published online on: February 14, 2012     https://doi.org/10.3892/ijo.2012.1372
  • Pages: 2022-2028
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Abstract

Breast cancer is one of the most common malignant tumors and the leading cause of mortality among women. In this study, we propose a human stem cell transplantation strategy, an important method for treating various cancers, as a potential breast cancer therapy. To this end, we used human amniotic membrane-derived epithelial stem cells (hAECs) as a cell source for performing human stem cell transplantation. hAECs have multipotent differentiation abilities and possess high proliferative potential. We transplanted hAECs into female BALB/c nude mice bearing tumors originating from MDA-MB-231 breast cancer cells. Co-culturred hAECs and MDA-MB-231 cells at a ratio of 1:4 or 1:8 (tumor cells to stem cells) inhibited breast cancer cell growth by 67.29 and 67.33%, respectively. In the xenograft mouse model, tumor volumes were significantly decreased by 5-flurouracil (5-FU) treatment and two different ratios of hAECs (1:4 and 1:8) by 84.33, 73.88 and 56.89%, respectively. Treatment of nude mice with hAECs (1:4) produced remarkable antitumor effects without any side-effects (e.g., weight loss, death and bruising) compared to the mice that received only 5-FU treatment. Tumor progression was significantly reduced by hAEC treatment compared to the xenograft model. On the other hand, breast tissues (e.g., the epidermis, dermis and reticular layer) appeared to be well-maintained following treatment with hAECs. Taken together, these results provide strong evidence that hAECs can be used as a safe and effective cancer-targeting cytotherapy for treating breast cancer.

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June 2012
Volume 40 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kang, N., Yi, B., Lim, S.Y., Hwang, K., Baek, Y.S., Kang, K., & Choi, K. (2012). Human amniotic membrane-derived epithelial stem cells display anticancer activity in BALB/c female nude mice bearing disseminated breast cancer xenografts. International Journal of Oncology, 40, 2022-2028. https://doi.org/10.3892/ijo.2012.1372
MLA
Kang, N., Yi, B., Lim, S. Y., Hwang, K., Baek, Y. S., Kang, K., Choi, K."Human amniotic membrane-derived epithelial stem cells display anticancer activity in BALB/c female nude mice bearing disseminated breast cancer xenografts". International Journal of Oncology 40.6 (2012): 2022-2028.
Chicago
Kang, N., Yi, B., Lim, S. Y., Hwang, K., Baek, Y. S., Kang, K., Choi, K."Human amniotic membrane-derived epithelial stem cells display anticancer activity in BALB/c female nude mice bearing disseminated breast cancer xenografts". International Journal of Oncology 40, no. 6 (2012): 2022-2028. https://doi.org/10.3892/ijo.2012.1372