Methanol extract of the ethnopharmaceutical remedy Smilax spinosa exhibits anti-neoplastic activity

  • Authors:
    • Mareike Seelinger
    • Ruxandra Popescu
    • Benedikt Giessrigl
    • Kanokwan  Jarukamjorn
    • Christine  Unger
    • Bruno Wallnöfer
    • Monika Fritzer-Szekeres
    • Thomas  Szekeres
    • Rene Diaz
    • Walter Jäger
    • Richard Frisch
    • Brigitte Kopp
    • Georg Krupitza
  • View Affiliations

  • Published online on: June 29, 2012     https://doi.org/10.3892/ijo.2012.1538
  • Pages: 1164-1172
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Plants have been the source of several effective drugs for the treatment of cancer and over 60% of anticancer drugs originate from natural sources. Therefore, extracts of the rhizome of Smilax spinosa, an ethnomedicinal plant from Guatemala which is used for the treatment of inflammatory conditions, were investigated regarding their anti-neoplastic activities. By using several solvents the methanol extract was by far the most potent against HL60 cell proliferation (50% inhibition at 60 µg/ml). Furthermore, fractionation of this extract yielded fraction F2, which exhibited enforced pro-apoptotic activity, and activated CYP1A1. Proteins that are relevant for cell cycle progression and apoptosis, as well as proto-oncogenes were investigated by western blotting. This revealed that the methanol extract increased the levels of p21 and this may have caused cell cycle attenuation. The derivative fraction F2 induced apoptosis through the intrinsic pathway, which correlated with the inhibition of Stat3 phosphorylation and concomitant induction of caspase 9, then caspase 8 and caspase 3. In summary, the methanol extract and the derivative fraction F2 of S. spinosa showed anti-neoplastic effects in HL-60 cells and CYP1A1 activation in estrogen receptor-positive MCF-7 breast cancer cells but not in estrogen-negative MDA-MB231 breast cancer cells. Based on our data Smilax spinosa may be a promising source for novel anticancer agents.
View Figures
View References

Related Articles

Journal Cover

September 2012
Volume 41 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Seelinger, M., Popescu, R., Giessrigl, B., Jarukamjorn, K., Unger, C., Wallnöfer, B. ... Krupitza, G. (2012). Methanol extract of the ethnopharmaceutical remedy Smilax spinosa exhibits anti-neoplastic activity. International Journal of Oncology, 41, 1164-1172. https://doi.org/10.3892/ijo.2012.1538
MLA
Seelinger, M., Popescu, R., Giessrigl, B., Jarukamjorn, K., Unger, C., Wallnöfer, B., Fritzer-Szekeres, M., Szekeres, T., Diaz, R., Jäger, W., Frisch, R., Kopp, B., Krupitza, G."Methanol extract of the ethnopharmaceutical remedy Smilax spinosa exhibits anti-neoplastic activity". International Journal of Oncology 41.3 (2012): 1164-1172.
Chicago
Seelinger, M., Popescu, R., Giessrigl, B., Jarukamjorn, K., Unger, C., Wallnöfer, B., Fritzer-Szekeres, M., Szekeres, T., Diaz, R., Jäger, W., Frisch, R., Kopp, B., Krupitza, G."Methanol extract of the ethnopharmaceutical remedy Smilax spinosa exhibits anti-neoplastic activity". International Journal of Oncology 41, no. 3 (2012): 1164-1172. https://doi.org/10.3892/ijo.2012.1538