Altered cardiac muscle mTOR regulation during the progression of cancer cachexia in the ApcMin/+ mouse

Manne,Nandini  D.P.K.; Lima,M.; Enos,R. T.; Wehner,P.; Carson,J. A.; Blough,Eric

doi:10.3892/ijo.2013.1893

Altered cardiac muscle mTOR regulation during the progression of cancer cachexia in the ApcMin/+ mouse

Authors:
- Nandini D.P.K. Manne
- M. Lima
- R. T. Enos
- P. Wehner
- J. A. Carson
- Eric Blough
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Affiliations: Center for Diagnostic Nanosystems, Marshall University, Huntington, WV, USA, Integrated Muscle Biology Laboratory, Applied Physiology Division Exercise Science, University of South Carolina, SC, USA, Department of Cardiology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV, USA
Published online on: April 11, 2013 https://doi.org/10.3892/ijo.2013.1893
Pages: 2134-2140

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Abstract

Cancer cachexia is a muscle wasting condition that occurs in response to a malignant growth in the body. The mechanisms regulating cardiac muscle mass with cachexia are not well understood. Using the ApcMin/+ mouse model of colorectal cancer, we investigated how cachexia affects the regulation of 5'-adenosine monophosphate-activated protein kinase (AMPK), protein kinase B (Akt) and mammalian target of rapamycin (mTOR) signaling in the heart. Compared to age-matched C57BL/6 (BL6) mice, ApcMin/+ body mass and heart mass were lower at 12 (11±5 and 8±3%, respectively) and 20 weeks (26±3 and 6±4%, respectively) of age (P<0.05). Diminished heart mass in the 20-week-old ApcMin/+ mice coincided with a decreased rate of myofibrillar protein synthesis and increased AMPKα phosphorylation. Cachexia decreased mTOR phosphorylation and the phosphorylation of the mTOR substrates, S6 ribosomal protein and 4EBP1 independent of Akt activation. These changes in mTOR-related protein signaling were accompanied by modest increases in the amount of Beclin1 but not protein ubiquitination or cardiomyocyte apoptosis. Taken together, these data suggest that loss of cardiac mass during cachexia progression in the ApcMin/+ mouse is associated with an Akt-independent suppression of anabolic signaling and evidence of increased autophagy.

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