Licochalcone A, a natural chalconoid isolated from Glycyrrhiza inflata root, induces apoptosis via Sp1 and Sp1 regulatory proteins in oral squamous cell carcinoma

  • Authors:
    • Jung Jae Cho
    • Jung-Il Chae
    • Goo Yoon
    • Ka Hwi Kim
    • Jin Hyoung Cho
    • Seung-Sik Cho
    • Young Sik Cho
    • Jung-Hyun Shim
  • View Affiliations

  • Published online on: May 23, 2014     https://doi.org/10.3892/ijo.2014.2461
  • Pages: 667-674
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Abstract

Licochalcone A (LCA), a chalconoid derived from root of Glycyrrhiza inflata, has been known to possess a wide range of biological functions such as antitumor, anti-angiogenesis, antiparasitic, anti-oxidant, antibacterial and anti-inflammatory effects. However, the anticancer effects of LCA on oral squamous cell carcinoma (OSCC) have not been reported. Our data showed that LCA inhibited OSCC cell (HN22 and HSC4) growth in a concentration- and time-dependent manner. Mechanistically, it was mediated via downregulation of specificity protein 1 (Sp1) expression and subsequent regulation of Sp1 downstream proteins such as p27, p21, cyclin D1, Mcl-1 and survivin. Here, we found that LCA caused apoptotic cell death in HSC4 and HN22 cells, as characterized by sub-G1 population, nuclear condensation, Annexin V staining, and multi-caspase activity and apoptotic regulatory proteins such as Bax, Bid, Bcl?xl, caspase-3 and PARP. Consequently, this study strongly suggests that LCA induces apoptotic cell death of OSCC cells via downregulation of Sp1 expression, prompting its potential use for the treatment of human OSCC.
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August-2014
Volume 45 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Cho JJ, Chae J, Yoon G, Kim KH, Cho JH, Cho S, Cho YS and Shim J: Licochalcone A, a natural chalconoid isolated from Glycyrrhiza inflata root, induces apoptosis via Sp1 and Sp1 regulatory proteins in oral squamous cell carcinoma. Int J Oncol 45: 667-674, 2014
APA
Cho, J.J., Chae, J., Yoon, G., Kim, K.H., Cho, J.H., Cho, S. ... Shim, J. (2014). Licochalcone A, a natural chalconoid isolated from Glycyrrhiza inflata root, induces apoptosis via Sp1 and Sp1 regulatory proteins in oral squamous cell carcinoma. International Journal of Oncology, 45, 667-674. https://doi.org/10.3892/ijo.2014.2461
MLA
Cho, J. J., Chae, J., Yoon, G., Kim, K. H., Cho, J. H., Cho, S., Cho, Y. S., Shim, J."Licochalcone A, a natural chalconoid isolated from Glycyrrhiza inflata root, induces apoptosis via Sp1 and Sp1 regulatory proteins in oral squamous cell carcinoma". International Journal of Oncology 45.2 (2014): 667-674.
Chicago
Cho, J. J., Chae, J., Yoon, G., Kim, K. H., Cho, J. H., Cho, S., Cho, Y. S., Shim, J."Licochalcone A, a natural chalconoid isolated from Glycyrrhiza inflata root, induces apoptosis via Sp1 and Sp1 regulatory proteins in oral squamous cell carcinoma". International Journal of Oncology 45, no. 2 (2014): 667-674. https://doi.org/10.3892/ijo.2014.2461