Buccal microRNA dysregulation in lung field carcinogenesis: Gender-specific implications

  • Authors:
    • Ramesh K. Wali
    • Thomas A. Hensing
    • Daniel W. Ray
    • Mart Dela Cruz
    • Ashish K. Tiwari
    • Andrew Radosevich
    • Lisa Jepeal
    • Hiran C. Fernando
    • Virginia R. Litle
    • Marjory Charlot
    • Navneet Momi
    • Vadim Backman
    • Hemant K. Roy
  • View Affiliations

  • Published online on: June 11, 2014     https://doi.org/10.3892/ijo.2014.2495
  • Pages: 1209-1215
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Abstract

MicroRNAs (miRNAs) have been shown to be reliable early biomarkers in a variety of cancers including that of lung. We ascertained whether the biomarker potential of miRNAs could be validated in microscopically normal and easily accessible buccal epithelial brushings from cigarette smokers as a consequence of lung cancer linked ‘field carcinogenesis’. We found that compared to neoplasia-free subjects, a panel of 68 miRNAs were upregulated and 3 downregulated in the normal appearing buccal mucosal cells collected from patients harboring lung cancer (n=76). The performance characteristics of selected miRNAs (with ≥1-fold change) were excellent with an average under the receiver operator characteristic curve (AUROC) of >0.80. Several miRNAs also displayed gender specificity between the groups. These results provide the first proof-of-concept scenario in which minimally intrusive cheek brushings could provide an initial screening tool in a large at-risk population.
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September-2014
Volume 45 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Wali RK, Hensing TA, Ray DW, Dela Cruz M, Tiwari AK, Radosevich A, Jepeal L, Fernando HC, Litle VR, Charlot M, Charlot M, et al: Buccal microRNA dysregulation in lung field carcinogenesis: Gender-specific implications. Int J Oncol 45: 1209-1215, 2014
APA
Wali, R.K., Hensing, T.A., Ray, D.W., Dela Cruz, M., Tiwari, A.K., Radosevich, A. ... Roy, H.K. (2014). Buccal microRNA dysregulation in lung field carcinogenesis: Gender-specific implications. International Journal of Oncology, 45, 1209-1215. https://doi.org/10.3892/ijo.2014.2495
MLA
Wali, R. K., Hensing, T. A., Ray, D. W., Dela Cruz, M., Tiwari, A. K., Radosevich, A., Jepeal, L., Fernando, H. C., Litle, V. R., Charlot, M., Momi, N., Backman, V., Roy, H. K."Buccal microRNA dysregulation in lung field carcinogenesis: Gender-specific implications". International Journal of Oncology 45.3 (2014): 1209-1215.
Chicago
Wali, R. K., Hensing, T. A., Ray, D. W., Dela Cruz, M., Tiwari, A. K., Radosevich, A., Jepeal, L., Fernando, H. C., Litle, V. R., Charlot, M., Momi, N., Backman, V., Roy, H. K."Buccal microRNA dysregulation in lung field carcinogenesis: Gender-specific implications". International Journal of Oncology 45, no. 3 (2014): 1209-1215. https://doi.org/10.3892/ijo.2014.2495