Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model

Yamazoe,Tomohiro; Koizumi,Shinichiro; Yamasaki,Tomohiro; Amano,Shinji; Tokuyama,Tsutomu; Namba,Hiroki

doi:10.3892/ijo.2014.2702

Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model

Authors:
- Tomohiro Yamazoe
- Shinichiro Koizumi
- Tomohiro Yamasaki
- Shinji Amano
- Tsutomu Tokuyama
- Hiroki Namba
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Affiliations: Department of Neurosurgery, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan
Published online on: October 8, 2014 https://doi.org/10.3892/ijo.2014.2702
Pages: 147-152

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Abstract

Although neural and mesenchymal stem cells have been well-known to have a strong glioma tropism, this activity in induced pluripotent stem cells (iPSCs) has not yet been fully studied. In the present study, we tested tumor tropic activity of mouse iPSCs and neural stem cells derived from the iPSC (iPS-NSCs) using in vitro Matrigel invasion chamber assay and in vivo mouse intracranial tumor model. Both iPSC and iPS-NSC had a similar potent in vitro tropism for glioma conditioned media. The migrated iPSCs to the gliomas kept expressing Nanog-GFP gene, suggesting no neuronal or glial differentiation. iPSCs or iPS-NSCs labeled with 5-bromo-2-deoxyuridine were intracranially implanted in the contralateral hemisphere to the GL261 glioma cell implantation in the allogeneic C57BL/6 mouse. Active migration of both stem cells was observed 7 days after implantation. Again, the iPSCs located in the tumor area expressed Nanog-GFP gene, suggesting that the migrated cells were still iPSCs. These findings demonstrated that both iPSCs and iPS-NSCs had potent glioma tropism and could be candidates as vehicles in stem cell-based glioma therapy.

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