N-cadherin promotes epithelial-mesenchymal transition and cancer stem cell-like traits via ErbB signaling in prostate cancer cells

  • Authors:
    • Min Wang
    • Dong Ren
    • Wei Guo
    • Shuai Huang
    • Zeyu Wang
    • Qiji Li
    • Hong Du
    • Libing Song
    • Xinsheng Peng
  • View Affiliations

  • Published online on: November 26, 2015     https://doi.org/10.3892/ijo.2015.3270
  • Pages: 595-606
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Abstract

N-cadherin has been reported to be upregulated and associated with metastasis and poor prognosis in prostate cancer patients, however the underlying mechanism still remains puzzling. In the present study, we found that upregulation of N-cadherin enhanced, while downregulation of N-cadherin impaired the invasion, migration, and epithelial to mesenchymal transition (EMT) of prostate cancer (PCa) cells. Overexpression of N-cadherin increased the efficiency of colony and tumor spheroid formation and the stemness factor expression (including c-Myc, Klf4, Sox2 and Oct4), and vice versa. Furthermore, microarray analysis and western blot analysis mechanistically proved that N-cadherin activated ErbB signaling pathway by upregulating the expression of Grb2, pShc and pERK1/2. Importantly, the regulation of N-cadherin on EMT and stemness was counteracted by lapatinib, a specific ErbB signaling pathway inhibitor. Collectively, these findings demonstrate that N-cadherin regulates EMT and stemness of PCa cells via activating ErbB signaling pathway, which indicates the pivotal role of N-cadherin/ErbB axis in the metastasis of prostate cancer.
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February 2016
Volume 48 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Wang, M., Ren, D., Guo, W., Huang, S., Wang, Z., Li, Q. ... Peng, X. (2016). N-cadherin promotes epithelial-mesenchymal transition and cancer stem cell-like traits via ErbB signaling in prostate cancer cells. International Journal of Oncology, 48, 595-606. https://doi.org/10.3892/ijo.2015.3270
MLA
Wang, M., Ren, D., Guo, W., Huang, S., Wang, Z., Li, Q., Du, H., Song, L., Peng, X."N-cadherin promotes epithelial-mesenchymal transition and cancer stem cell-like traits via ErbB signaling in prostate cancer cells". International Journal of Oncology 48.2 (2016): 595-606.
Chicago
Wang, M., Ren, D., Guo, W., Huang, S., Wang, Z., Li, Q., Du, H., Song, L., Peng, X."N-cadherin promotes epithelial-mesenchymal transition and cancer stem cell-like traits via ErbB signaling in prostate cancer cells". International Journal of Oncology 48, no. 2 (2016): 595-606. https://doi.org/10.3892/ijo.2015.3270