Open Access

Identifying molecular subtypes in human colon cancer using gene expression and DNA methylation microarray data

  • Authors:
    • Zhonglu Ren
    • Wenhui Wang
    • Jinming Li
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  • Published online on: November 24, 2015     https://doi.org/10.3892/ijo.2015.3263
  • Pages: 690-702
  • Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Identifying colon cancer subtypes based on molecular signatures may allow for a more rational, patient-specific approach to therapy in the future. Classifications using gene expression data have been attempted before with little concordance between the different studies carried out. In this study we aimed to uncover subtypes of colon cancer that have distinct biological characteristics and identify a set of novel biomarkers which could best reflect the clinical and/or biological characteristics of each subtype. Clustering analysis and discriminant analysis were utilized to discover the subtypes in two different molecular levels on 153 colon cancer samples from The Cancer Genome Atlas (TCGA) Data Portal. At gene expression level, we identified two major subtypes, ECL1 (expression cluster 1) and ECL2 (expression cluster 2) and a list of signature genes. Due to the heterogeneity of colon cancer, the subtype ECL1 can be further subdivided into three nested subclasses, and HOTAIR were found upregulated in subclass 2. At DNA methylation level, we uncovered three major subtypes, MCL1 (methylation cluster 1), MCL2 (methylation cluster 2) and MCL3 (methylation cluster 3). We found only three subtypes of CpG island methylator phenotype (CIMP) in colon cancer instead of the four subtypes in the previous reports, and we found no sufficient evidence to subdivide MCL3 into two distinct subgroups.
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February 2016
Volume 48 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Ren, Z., Wang, W., & Li, J. (2016). Identifying molecular subtypes in human colon cancer using gene expression and DNA methylation microarray data. International Journal of Oncology, 48, 690-702. https://doi.org/10.3892/ijo.2015.3263
MLA
Ren, Z., Wang, W., Li, J."Identifying molecular subtypes in human colon cancer using gene expression and DNA methylation microarray data". International Journal of Oncology 48.2 (2016): 690-702.
Chicago
Ren, Z., Wang, W., Li, J."Identifying molecular subtypes in human colon cancer using gene expression and DNA methylation microarray data". International Journal of Oncology 48, no. 2 (2016): 690-702. https://doi.org/10.3892/ijo.2015.3263