Human rhabdomyosarcoma cells express functional pituitary and gonadal sex hormone receptors: Therapeutic implications

Poniewierska-Baran,Agata; Schneider,Gabriela; Sun,Wenyue; Abdelbaset-Ismail,Ahmed; Barr,Frederic G.; Ratajczak,Mariusz Z.

doi:10.3892/ijo.2016.3439

Human rhabdomyosarcoma cells express functional pituitary and gonadal sex hormone receptors: Therapeutic implications

Authors:
- Agata Poniewierska-Baran
- Gabriela Schneider
- Wenyue Sun
- Ahmed Abdelbaset-Ismail
- Frederic G. Barr
- Mariusz Z. Ratajczak
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Affiliations: Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA, Department of Regenerative Medicine, Warsaw Medical University, Warsaw, Poland, Laboratory of Pathology, National Cancer Institute, Bethesda, MD, USA
Published online on: March 11, 2016 https://doi.org/10.3892/ijo.2016.3439
Pages: 1815-1824
Copyright: © Poniewierska-Baran et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Evidence has accumulated that sex hormones play an important role in several types of cancer. Because they are also involved in skeletal muscle development and regeneration, we were therefore interested in their potential involvement in the pathogenesis of human rhabdomyosarcoma (RMS), a skeletal muscle tumor. In the present study, we employed eight RMS cell lines (three fusion positive and five fusion negative RMS cell lines) and mRNA samples obtained from RMS patients. The expression of sex hormone receptors was evaluated by RT-PCR and their functionality by chemotaxis, adhesion and direct cell proliferation assays. We report here for the first time that follicle-stimulating hormone (FSH) and luteinizing hormone (LH) receptors are expressed in established human RMS cell lines as well as in primary tumor samples isolated from RMS patients. We also report that human RMS cell lines responded both to pituitary and gonadal sex hormone stimulation by enhanced proliferation, chemotaxis, cell adhesion and phosphorylation of MAPKp42/44 and AKT. In summary, our results indicate that sex hormones are involved in the pathogenesis and progression of RMS, and therefore, their therapeutic application should be avoided in patients that have been diagnosed with RMS.

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