Repression of Smad4 by miR‑205 moderates TGF-β-induced epithelial-mesenchymal transition in A549 cell lines

Corrigendum in: /10.3892/ijo.2021.5166

  • Authors:
    • Yuanyuan Zeng
    • Jianjie Zhu
    • Dan Shen
    • Hualong Qin
    • Zhe Lei
    • Wei Li
    • Jian-An Huang
    • Zeyi Liu
  • View Affiliations

  • Published online on: May 31, 2016     https://doi.org/10.3892/ijo.2016.3547
  • Pages: 700-708
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Abstract

The TGF-β/Smad signaling pathway plays important roles in cancer cell proliferation, apoptosis, differentiation, angiogenesis and epithelial-mesenchymal transition (EMT), which is the key event in the early stages of cancer metastasis and enhances the capability of cell migration and invasion. Smad4 acts as the only Co-Smad of TGF/Smad signaling pathway and plays the key role in TGF-β-mediated EMT. Nevertheless, the mRNA regulation mechanisms of Smad4 in human non-small cell lung cancer (NSCLC) remains largely unclear. Computational algorithms predicted that the 3'-UTR of Smad4 is a target of miR‑205. Here, we validated that miR‑205 could directly bind to 3'-UTR of Smad4 by luciferase assays. Moreover, we investigated the functional roles of miR‑205 and its molecular link to Smad4 in lung cancer cells. In this study, we confirmed that overexpression of miR‑205 suppressed the expression of Smad4, in turn, weakened the TGF-β/Smad signaling pathway and inhibited TGF-β/Smad4-induced EMT, invasion and migration ultimately. Furthermore, this study shows that miR‑205 can serve as a promising therapeutic target of highly aggressive NSCLC.
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August-2016
Volume 49 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zeng Y, Zhu J, Shen D, Qin H, Lei Z, Li W, Huang J and Liu Z: Repression of Smad4 by miR‑205 moderates TGF-β-induced epithelial-mesenchymal transition in A549 cell lines Corrigendum in /10.3892/ijo.2021.5166. Int J Oncol 49: 700-708, 2016.
APA
Zeng, Y., Zhu, J., Shen, D., Qin, H., Lei, Z., Li, W. ... Liu, Z. (2016). Repression of Smad4 by miR‑205 moderates TGF-β-induced epithelial-mesenchymal transition in A549 cell lines Corrigendum in /10.3892/ijo.2021.5166. International Journal of Oncology, 49, 700-708. https://doi.org/10.3892/ijo.2016.3547
MLA
Zeng, Y., Zhu, J., Shen, D., Qin, H., Lei, Z., Li, W., Huang, J., Liu, Z."Repression of Smad4 by miR‑205 moderates TGF-β-induced epithelial-mesenchymal transition in A549 cell lines Corrigendum in /10.3892/ijo.2021.5166". International Journal of Oncology 49.2 (2016): 700-708.
Chicago
Zeng, Y., Zhu, J., Shen, D., Qin, H., Lei, Z., Li, W., Huang, J., Liu, Z."Repression of Smad4 by miR‑205 moderates TGF-β-induced epithelial-mesenchymal transition in A549 cell lines Corrigendum in /10.3892/ijo.2021.5166". International Journal of Oncology 49, no. 2 (2016): 700-708. https://doi.org/10.3892/ijo.2016.3547