CD44-shRNA recombinant adenovirus inhibits cell proliferation, invasion, and migration, and promotes apoptosis in HCT116 colon cancer cells

  • Authors:
    • Soo Young Lee
    • Kyung Ae Kim
    • Chang Hyun Kim
    • Young Jin Kim
    • Jae-Hyuk Lee
    • Hyeong Rok Kim
  • View Affiliations

  • Published online on: December 9, 2016     https://doi.org/10.3892/ijo.2016.3801
  • Pages: 329-336
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Abstract

The cell-surface glycoprotein CD44 is closely associated with cell proliferation, tumor invasion, and metastasis. Previous studies have reported that knockdown of CD44 with short hairpin RNA (shRNA) reduced cell proliferation and migration, and induced apoptosis. However, more efficient means of delivering small interference RNA are still necessary. We developed an in vitro model of CD44-shRNA recombinant adenovirus (Ad-CD44-shRNA) and evaluated its ability to alter tumor invasion, migration, and apoptosis in human colon cancer cells. An shRNA against CD44 was used for knockdown of CD44 expression, and recombinant adenovirus was constructed using AD293 cells. The Ad-CD44-shRNA-treated HCT116 colon cancer cells showed a significant decrease in cell proliferation, migration, and invasion, while apoptosis was increased. The Ad-CD44-shRNA also decreased the phosphorylation of Akt and GSK-3β. The levels of Bcl-2 and Bcl-xL expression were downregulated, whereas the expression levels of Bax, cleaved caspase‑3 and -9, and PARP were increased in Ad-CD44-shRNA-treated colon cancer cells. These results support the feasibility of an adenovirus-mediated RNA interference therapy targeting human colon cancer via the CD44 as a potential future therapeutic intervention.
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January-2017
Volume 50 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lee SY, Kim KA, Kim CH, Kim YJ, Lee J and Kim HR: CD44-shRNA recombinant adenovirus inhibits cell proliferation, invasion, and migration, and promotes apoptosis in HCT116 colon cancer cells. Int J Oncol 50: 329-336, 2017
APA
Lee, S.Y., Kim, K.A., Kim, C.H., Kim, Y.J., Lee, J., & Kim, H.R. (2017). CD44-shRNA recombinant adenovirus inhibits cell proliferation, invasion, and migration, and promotes apoptosis in HCT116 colon cancer cells. International Journal of Oncology, 50, 329-336. https://doi.org/10.3892/ijo.2016.3801
MLA
Lee, S. Y., Kim, K. A., Kim, C. H., Kim, Y. J., Lee, J., Kim, H. R."CD44-shRNA recombinant adenovirus inhibits cell proliferation, invasion, and migration, and promotes apoptosis in HCT116 colon cancer cells". International Journal of Oncology 50.1 (2017): 329-336.
Chicago
Lee, S. Y., Kim, K. A., Kim, C. H., Kim, Y. J., Lee, J., Kim, H. R."CD44-shRNA recombinant adenovirus inhibits cell proliferation, invasion, and migration, and promotes apoptosis in HCT116 colon cancer cells". International Journal of Oncology 50, no. 1 (2017): 329-336. https://doi.org/10.3892/ijo.2016.3801