Rb deficiency accelerates progression of carcinoma of the urinary bladder in vivo and in vitro through inhibiting autophagy and apoptosis

  • Authors:
    • Cheng-Yuan Wang
    • Zhi-Bin Xu
    • Jiang-Ping Wang
    • Yong Jiao
    • Bo Zhang
  • View Affiliations

  • Published online on: February 22, 2017     https://doi.org/10.3892/ijo.2017.3889
  • Pages: 1221-1232
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Abstract

Urinary bladder cancer is known as a common cancer diagnosed across the world and results in significant mortality and morbidity rates among patients. The retinoblastoma (Rb) protein, as a main tumor suppressor, controls cellular responses to potentially oncogenic stimulation. Rb phosphorylation could disrupt E2F complex formation, resulting in diverse transcription factor dysfunction. In our study, we investigated how Rb is involved in controlling urinary bladder cancer progression. The results indicate that Rb expression is reduced in mice with urinary bladder tumor, and its suppression leads to urinary bladder cancer progression in vivo and in vitro. Rb mutation directly results in tumor size with lower survival rate in vivo. Rb knockdown in vitro promoted bladder tumor cell proliferation, migration and invasion. Interestingly, Rb knockout and knockdown result in autophagy and apoptosis inhibition via suppressing p53 and caspase-3 signaling pathways, enhancing bladder cancer development in vitro and in vivo. These findings reveal that Rb deficiency accelerated urinary bladder cancer progression, exposing an important role of Rb in suppressing urinary bladder cancer for treatment in the future.
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April-2017
Volume 50 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Wang C, Xu Z, Wang J, Jiao Y and Zhang B: Rb deficiency accelerates progression of carcinoma of the urinary bladder in vivo and in vitro through inhibiting autophagy and apoptosis. Int J Oncol 50: 1221-1232, 2017
APA
Wang, C., Xu, Z., Wang, J., Jiao, Y., & Zhang, B. (2017). Rb deficiency accelerates progression of carcinoma of the urinary bladder in vivo and in vitro through inhibiting autophagy and apoptosis. International Journal of Oncology, 50, 1221-1232. https://doi.org/10.3892/ijo.2017.3889
MLA
Wang, C., Xu, Z., Wang, J., Jiao, Y., Zhang, B."Rb deficiency accelerates progression of carcinoma of the urinary bladder in vivo and in vitro through inhibiting autophagy and apoptosis". International Journal of Oncology 50.4 (2017): 1221-1232.
Chicago
Wang, C., Xu, Z., Wang, J., Jiao, Y., Zhang, B."Rb deficiency accelerates progression of carcinoma of the urinary bladder in vivo and in vitro through inhibiting autophagy and apoptosis". International Journal of Oncology 50, no. 4 (2017): 1221-1232. https://doi.org/10.3892/ijo.2017.3889