PRL-3 improves colorectal cancer cell proliferation and invasion through IL-8 mediated glycolysis metabolism

  • Authors:
    • Heyang Xu
    • Yujie Zeng
    • Lu Liu
    • Qian Gao
    • Shaowen Jin
    • Qiusheng Lan
    • Wei Lai
    • Xingxi Luo
    • Heng Wu
    • Yongliang Huang
    • Zhonghua Chu
  • View Affiliations

  • Published online on: August 2, 2017     https://doi.org/10.3892/ijo.2017.4090
  • Pages: 1271-1279
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Abstract

Phosphatase of regenerating liver-3 (PRL-3) has been found to be overexpressed in liver metastases of colorectal cancer and rarely expressed in primary tumors, which plays an important role in the metastasis of colorectal cancer cells. Metabolism reprogramming has been found to be a hallmark of cancer cells, and aerobic glycolysis is a metabolic adaption for cancer cells and promotes cell proliferation. However, the association between PRL-3 and glycolysis in colorectal cancer cells is not well understood. In the present study, we explored the association between PRL-3 and glycolysis. We found that PRL-3 improved colorectal cancer cell glucose assumption, lactate production and reduced intracellular ROS levels. Besides, PRL-3 improved the expression of Glut1, HK2, PKM2 and LDHA, which are important glycolysis related molecules and enzymes. Moreover, we explored IL-8 mediated enhancement of glycolysis by PRL-3. More importantly, the proliferation and invasion of colorectal cancer cells were enhanced significantly by PRL-3 through improving glycolysis. Taken together, these results implicated the important role of PRL-3 in glycolysis metabolism through improving IL-8 secretion in colorectal cancer cells, and PRL-3 mediated glycolysis contributed to the promotion of cancer metastasis.

Introduction

Colorectal cancer is one of the most common cancers in the world. Patients in advanced stages with metastatic lesions appear to have a poor prognosis (1). Clinical studies have found that colorectal cancer cells prefer to metastasize to the liver over other organs, which results in poor prognosis. Therefore, it is important to identify and understand the factors involved in the progression of colorectal cancer metastasis to the liver.

PRL-3 belongs to the family of protein tyrosine phosphatases (PTPs), which has been demonstrated to play an important role in colorectal cancer metastasis in the liver (2). PTPs regulate phosphorylation of many important signaling molecules that are involved in cell proliferation, migration and prognosis (3). PRL-3 is normally expressed in heart and skeletal muscle. However, studies have found that PRL-3 is significantly overexpressed in metastatic cells and is moderately expressed in primary lesions of colorectal cancer (4). Moreover, the expression of PRL-3 in primary colorectal cancer lesions indicates poor prognosis and shortened survival (5). Therefore, PRL-3 appears to be a biomarker for colorectal cancer, and, in particular, a biomarker for colorectal cancer liver metastasis. However, the mechanisms of regulating liver metastasis are still uncertain. Our previous research found that PRL-3 promoted colorectal cancer cell proliferation through TNF-α secretion, which also induced the activation of a Ca2+-activated K+ channel (KCNN4) (6). We demonstrated that PRL-3 facilitated epithelial mesenchymal transition (EMT) in colorectal cancer cells (7), indicating that PRL-3 strongly influenced the biological characteristics of tumor cells. Moreover, we studied the tumor microenvironment and found that PRL-3 integrated with tumor associated macrophages (TAMs), induced TAM secreting inflammatory cytokines such as IL-6 and IL-8, which then enhanced colorectal cancer cell invasion (8).

Tumor cell growth and proliferation require large quantities of bioenergy and biomaterials. In recent years, increasing number of studies have found that an important hallmark of cancer cells is metabolism reprogramming, which was first proposed by Warburg (termed the Warburg effect) in the 1920s (9,10). Unlike normal cells, most cancer cells exhibit a high rate of glycolysis rather than oxidative phosphorylation and therefore produce large amounts of lactate, leading to a decrease in extracellular pH, which facilitates cancer cell removal (11). The activation of glycolytic pathways promotes tumor cells to adapt to fast proliferation. Furthermore, glycolysis-associated enzymes and molecules are highly expressed in tumor cells, which also play an important role in cancer (12).

Inflammation is an important risk factor for colorectal cancer, but the mechanisms underlying this effect of inflammation on colorectal cancer cells are still not fully understood. It has been indicated that tumor associated inflammation may affect the proliferation, metastasis and angiogenesis of tumor cells (13). Notably, our previous study also found that inflammatory cytokines IL-6 and IL-8, which were secreted by TAMs, enhanced colorectal cancer cells invasion (8). However, the association between inflammatory cytokines and glycolysis metabolism is still uncertain.

In the present study, we aimed to determine whether PRL-3 is involved in the metabolism reprogramming of colorectal cancer cells. This investigation revealed that PRL-3 promotes glycolysis through secretion of IL-8 in colorectal cancer cells, leading to an increase of glucose consumption and lactate production, reduced intercellular reactive oxygen species (ROS) levels and induced overexpression of glycolysis enzymes and molecules, contributing to enhanced tumor cell proliferation and invasion.

Materials and methods

Samples and patients

Colorectal cancer cell samples were collected from 47 patients admitted to the Department of Gastroenteropancreatic Surgery of Sun Yat-sen Memorial Hospital, Sun Yat-sen University, between 2013 and 2016. Specimens were collected immediately after tumor removal. All samples were collected with informed consent according to the Internal Review and the Ethics Boards of the Sun Yat-sen Memorial Hospital of Sun Yat-sen University. The protocol was approved by the Ethics Committee of Sun Yat-sen Memorial Hospital.

Cell cultures and treatments

LoVo colorectal cancer cells were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were transfected with PAcGFP-PRL-3 (LoVo-P) or PAcGFP (LoVo-C) using Lipofectamine 3000. Cells were stored at Sun Yat-sen Memorial Hospital (6). Cells were cultured in RPMI-1640 medium and 10% fetal bovine serum (FBS), with 100 mg/ml penicillin. The cells were incubated at 37°C, 5% CO2 in a humidified atmosphere.

Reagents and antibodies

Lipofectamine 3000 was purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was purchased from Biological Industries (Kibbutz Beit Haemek, Israel). RPMI was purchased from Invitrogen (Carlsbad, CA, USA). TRIzol and Prime Script RT were purchased from Takara Bio (Dalian, China). The siRNA was purchased from Shanghai GenePharma, Co., Ltd. (Shanghai, China). Antibodies against GAPDH (cat. no. ab8245), IL-8 (cat. no. ab18672), Glut1 (cat. no. ab115730), PKM2 (cat. no. ab38237), HK2 (cat. no. ab104836), LDHA (cat. no. ab125683) were purchased from Abcam (Cambridge, MA, USA).

Western blot assay

Cells were washed with phosphate-buffered saline (PBS) and then lysed on ice with RIPA buffer containing 1% PMSF. The Bradford assay was used to detect protein concentration. Denatured proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to PVDF membranes and then blocked in 5% non-fat milk. Membranes were washed 3 times with Tris-buffered saline + 0.1% Tween-20 (TBST), incubated overnight at 4°C with relevant primary antibodies, and then washed and incubated with horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. Labeled proteins and relative band intensities were visualized and measured with Quantity One software (Bio-Rad Laboratories, Hrercules, CA, USA).

RNA extraction and real-time quantitative RT-PCR

Total RNA was isolated using TRIzol reagent, and 500 ng RNA was reverse transcribed using PrimeScript RT according to the manufacturer's protocol. Quantitative real-time RT-PCR was performed using the LightCycler 480 (Roche, Basel, Switzerland) and SYBR assays (Takara Bio). Primers were designed to detect GAPDH, PRL-3, Glut1, PKM2, HK2, LDHA and IL-8. The primers used for qRT-PCR are shown in Table I. Each sample contained 1X SYBR Premix Ex Taq™, 0.2 μM of each forward and reverse primer and 500 ng template cDNA in a final volume of 20 μl. Cycling parameters were set as follows: denaturation at 95°C for 30 sec, followed by 40 amplification cycles (95°C for 5 sec and 60°C for 20 sec). For relative quantification, 2−ΔCt was used to calculate the fold change in gene expression. All experiments were performed in triplicate.

Table I

Oligonucleotide sequence of qRT-PCR primers.

Table I

Oligonucleotide sequence of qRT-PCR primers.

GeneForward primerReverse primerAmplicon
GAPDH AATGGGCAGCCGTTAGGAAA GCGCCCAATACGACCAAATC168
PRL-3 ACACATGCGCTTCCTCATCA GTCACTTCACACACACGCAC111
Glut1 GGCTTCTCCAACTGGACCTC CCGGAAGCGATCTCATCGAA176
HK2 CAAGAAGCTCCCACTGGGTT CAACGTCTCTGCCTTCCACT122
PKM2 GTCTGGGAGGAAAGTCGCTC GGCGGAAGGACACAGATTCA104
LDHA CATGGCCTGTGCCATCAGTA AGATATCCACTTTGCCAGAGACA158
IL-8 CCACCGGAAGGAACCATCTC TTCCTTGGGGTCCAGACAGA279
Glycolysis consumption and lactate production

Glucose and lactate assay kits were purchased from Sigma-Aldrich to determine the concentrations of glucose and lactate in the culture medium, respectively. Cells were seeded on 6-well plates at a density of 1×105 cells/well and the medium was changed to DMEM after incubation overnight. The concentrations of glucose and lactate were measured according to the manufacturer's protocol.

Measurement of intracellular reactive oxygen species (ROS)

Intracellular ROS levels were detected by H2DCF-DA (Invitrogen). The cells were cultured in a 96-well plate. Cells were washed with PBS before incubation with H2DCF-DA for 30 min, and ROS levels were examined at excitation and emission wavelength of 485 and 520 nm. Cell numbers were normalized before the measurement.

Inflammatory cytokine array analysis

The RayBio Cytokine Analysis array (RayBiotech, Norcross, GA, USA), consisting of 40 different inflammatory cytokine antibodies spotted onto a membrane, was used in the present study. Cytokine array membranes were blocked for 30 min and then incubated with samples at 37°C for 1 h. Then membranes were washed and incubated with diluted biotin-conjugated antibodies at 37°C for 2 h. After the membranes were washed, 1,000-fold diluted horseradish peroxidase-conjugated streptavidin was added and incubation was continued for 2 h. Membranes were then washed thoroughly and exposed to detection buffer in the dark. By comparing the signal intensities, relative expression levels of cytokines were made. The intensities of signals were quantified by densitometry.

Immunohistochemistry

Using primary antibodies against PRL-3, Glut1, PKM2, HK2, LDHA and IL-8, the tissue slides were incubated overnight at room temperature. Secondary staining with Alexa Fluor 555 conjugated donkey anti-rabbit and Alexa Fluor 488 conjugated goat anti-mouse secondary antibodies was performed at room temperature for 60 min. Images were taken with a Zeiss LSM 700 laser scanning microscope (Carl Zeiss, Oberkochen, Germany) with a core data acquisition system (Applied Precision, Bratislava, Slovakia). For control experiments, primary antibody was substituted with normal rabbit serum.

Wound scratch test and invasion assays

Cell migration was measured by the movement of cells into a scraped area created by the tip of a 200 μl pipette. The degree of 'wound closure' was examined after 24 and 48 h. After cell adherence, the remaining gap was then measured using light microscopy and quantified. Invasion assays were performed using 105 cells/well added to a Matrigel invasion chamber. FBS was added to the lower chamber, and the number of cells invaded from the top chamber after 24 and 48 h was measured with a spectrophotometer.

Statistical analysis

Statistical analysis was performed using the SPSS. Data from three separate experiments were reported as the means ± SD. Statistical significance between the samples was assessed by the Student's-t test where P<0.05 was considered to be statistically significant.

Results

PRL-3 promotes glycolysis in colorectal cancer cells

Tumor cells reprogram their metabolism from oxidative phosphorylation to glycolysis to meet huge energy demands and for large amounts of biomass (14). A glycolytic feature is often characterized by glucose assumption, lactate production and reduced intracellular ROS levels. To examine the role of PRL-3 in glycolysis of colorectal cancer cells, we used glucose consumption assays, lactate production assays and intracellular ROS measurement assays to analyze glycolysis in PRL-3 overexpressed colorectal cancer cells (LoVo-P), control cells (LoVo-C) and wild-type cells (LoVo). We found that overexpression of PRL-3 promoted glucose consumption, lactate production and reduced the intracellular ROS levels. However, LoVo-C and LoVo cells did not exhibit the same trends (Fig. 1).

PRL-3 promotes Glut1, HK2, PKM2 and LDHA expression in colorectal cancer cells

The progression of glycolysis contains more than ten metabolic reactions that are catalyzed by a number of enzymes or molecules. For example, Glut1 transports glucose across the plasma membrane, which plays an important role in rate-limiting glucose metabolism. Additionally, HK2, PKM2 and LDHA are important rate-limiting enzymes in glycolysis. To examine whether glycolysis-associated molecules and enzymes may be regulated by PRL-3 in colorectal cancer cells, we analyzed mRNA expressions of Glut1, HK2, PKM2 and LDHA. Compared to LoVo-C and LoVo cells, the data showed that expression of Glut1, HK2, PKM2 and LDHA were all increased in LoVo-P cells (Fig. 2A). Consistent with these results, western blot analysis also showed the same trends (Fig. 2B and C).

PRL-3 improves IL-8 expression in colorectal cancer cells

We next explored the inflammatory cytokine expression in supernatants by using inflammatory cytokine antibody array to find the expression differences between LoVo-P, LoVo-C and LoVo cells. Each membrane contained 40 inflammatory cytokines (Table II), relative inflammatory cytokine expression was compared between between LoVo-P, LoVo-C and LoVo cells separately. Results showed significant upregulation of IL-8 expression in LoVo-P cells (Fig. 3A). RT-PCR showed that IL-8 gene was upregulated in LoVo-P cells (Fig. 3B). In line with the mRNA level, the protein level of IL-8 showed the same trends (Fig. 3C and D). These data suggested that PRL-3 improved the expression of IL-8 in colorectal cancer cells.

Table II

Inflammatory cytokines on the membrane.

Table II

Inflammatory cytokines on the membrane.

ABCDEFGHIJKL
1POSPOSNEGNEGEotaxinEotaxin-2G-CSFGM-CSFICAM1IFN-γI-309IL-1α
2POSPOSNEGNEGEotaxinEotaxin-2G-CSFGM-CSFICAM1IFN-γI-309IL-1α
3IL-1βIL-2IL-3IL-4IL-6IL-6sRIL-7IL-8IL-10IL-11IL-12p40IL-12p70
4IL-1βIL-2IL-3IL-4IL-6IL-6sRIL-7IL-8IL-10IL-11IL-12p40IL-12p70
5IL-13IL-15IL-16IL-17IP-10MCP-1MCP-2M-CSFMIGMIP-1αMIP-1βMIP-1δ
6IL-13IL-15IL-16IL-17IP-10MCP-1MCP-2M-CSFMIGMIP-1αMIP-1βMIP-1δ
7RANTESTGF-βTNF-αTNF-βsTNF-RIsTNF-RIIPDGF-BBTIMP-2BLANKBLANKNEGPOS
8RANTESTGF-βTNF-αTNF-βsTNF-RIsTNF-RIIPDGF-BBTIMP-2BLANKBLANKNEGPOS
IL-8 mediates the promotion of glycolysis in colorectal cancer cells

To explore whether IL-8 mediates the promotion of glycolysis, we used anti-IL-8 antibody to neutralize IL-8 function. The addition of anti-IL-8 antibody to the culture medium increased the remaining glucose, reduced the lactate production and increased intracellular ROS levels of cancer cells in a dose-dependent manner, whereas an isotype-matched IgG (10 μg/ml) did not have same effects (Fig. 4A–C), indicating the role of IL-8 in colorectal cancer cells glycolysis. In order to examine whether PRL-3 improves glycolysis related molecules and enzymes through IL-8, we examined the effect of IL-8 on the expression of Glut1, HK2, PKM2 and LDHA in LoVo-P cells. RT-PCR and western blot analysis showed these molecules and enzymes were significantly reduced after anti-IL-8 antibody was added into the culture medium (Fig. 4D and F).

PRL-3 improves growth and invasion via glycolysis in colorectal cancer cells

Lactate and glycolysis-associated enzymes and molecules have been found to play important roles in improving cancer cell metastasis (1519). To identify the role of PRL-3 induced glycolysis through IL-8 on colorectal cancer cell growth and invasion, we inhibited lactate by pretreating colorectal cancer cells with oxamic acid, or we inhibited Glut1, HK2, PKM2 or LDHA expression by siRNA, or we inhibited IL-8 by pretreating anti-IL-8 antibody. We found that LoVo-P cells exhibited decreased motility and invasion when oxamic acid or anti-IL-8 antibody was added (Fig. 5A–D). Moreover, our data also showed decreased motility and invasion of LoVo-P cells when the expression of Glut1, HK2, PKM2 or LDHA was inhibited (Fig. 5E–H).

Correlation between PRL-3 and Glut1, HK2, PKM2, LDHA and IL-8 in CRC patients

To explore the association between PRL-3, IL-8, Glut1, HK2, PKM2 and LDHA in clinical patient tissues, we performed IHC and scored the results of 47 patients with colorectal cancer. We first analyzed the expression of PRL-3 in tissues from clinical colorectal carcinoma samples. Consistent with our previous research, PRL-3 was rarely expressed in adjacent normal colorectal lesions but was overexpressed in colorectal carcinomas lesions. Furthermore, we analyzed the protein expression of Glut1, HK2, PKM2, LDHA and IL-8 and found that they were overexpressed in tumor tissues and positively correlated with PRL-3 expression (Fig. 6A). IHC scores of PRL-3, Glut1, HK2, PKM2, LDHA and IL-8 were remarkably higher in tumor tissues than those in normal adjacent tissues (Fig. 6B and Table III).

Table III

Association of PRL-3 and Glut1, HK2, PKM2, LDHA, IL-8 expression in 47 colorectal cancer patients.

Table III

Association of PRL-3 and Glut1, HK2, PKM2, LDHA, IL-8 expression in 47 colorectal cancer patients.

Adjacent tissuesTumor tissues
Number4747
PRL-3 expression
 No staining30
 Weak staining440
 Intermediate staining014
 Strong staining043
 IHC score, mean ± SE3.0±1.39.8±2.1
 P-value<0.05
Glut1 expression
 No staining00
 Weak staining374
 Intermediate staining106
 Strong staining037
 IHC score, mean ± SE3.4±1.79.9±2.0
 P-value<0.05
HK2 expression
 No staining00
 Weak staining201
 Intermediate staining2710
 Strong staining036
 IHC score, mean ± SE4.9±2.110.1±2.0
 P-value<0.05
PKM2 expression
 No staining00
 Weak staining340
 Intermediate staining139
 Strong staining038
 IHC score, mean ± SE3.7±1.79.9±2.0
 P-value<0.05
LDHA expression
 No staining00
 Weak staining250
 Intermediate staining2214
 Strong staining033
 IHC score, mean ± SE4.4±2.59.9±2.2
 P-value<0.05
IL-8 expression
 No staining00
 Weak staining440
 Intermediate staining310
 Strong staining037
 IHC score, mean ± SE3.1±1.29.4±1.6
 P-value<0.05

Discussion

Reprogrammed metabolism, which fuels tumor cells replication, growth and invasion, was added to the hallmarks of cancer (20). Many studies have explored the mechanisms of tumor cell unlimited growth and altered metabolism. In the present study, we found that PRL-3 improves glycolysis of colorectal cancer cells, which contributes to cancer cells proliferation and invasion in vitro. Our previous research revealed that inflammatory cytokine IL-8, which was secreted by tumor associated macrophage, promoted colorectal cancer cell invasion (8). Our current experiments showed that PRL-3 improved IL-8 expression in colorectal cancer cells, and IL-8 participates in the promotion of glycolysis by PRL-3. To the best of our knowledge, this is the first report indicating the association between PRL-3 and tumor metabolism reprogram, furthermore, our research uncovered the role of inflammatory cytokine IL-8 in glycolysis.

Various research has been made into the key steps of metastatic process influenced by PRL-3. For example, PRL-3 repressed various target genes which participate in cell cycle arrest to give cell unlimited proliferative advantage (21). PRL-3 promotes PI3K-AKT activity, which is an important driver of cell proliferation and survival (22). PRL-3 has been associated in regulation of focal adhesion components, such as Src, integrin and paxillin, which induced cell motility (23). PRL-3 promoted cell invasion by increasing MMP2 activity (23), induced EMT by acting upstream of PI3K/AKT signaling (24). PRL-3 increased the expression of VEGF and promoted tumor cell angiogenesis (25). However, little attention has been given to the relationship between PRL-3 and tumor metabolism reprogram. In the present study, remaining glucose in the culture medium of LoVo-P cells is less than LoVo and LoVo-C cells, which means LoVo-P cells consumed more glucose. The culture medium did not contain FBS to eliminate possible interference caused by cell growth rate. Besides, lactate production level was significantly higher and intracellular ROS level was lower in LoVo-P cells, indicating the function of PRL-3 in improving colorectal cancer cell glycolysis. Moreover, we also found the expression of glycolysis related molecules and enzymes Glut1, HK2, PKM2 and LDHA were increased in LoVo-P cells, these findings provide evidence that PRL-3 promotes colorectal cancer cell glycolysis.

Our previous research showed the association between PRL-3 and inflammation in tumor microenvironment. Tumor associated macrophages secreted IL-6 and IL-8 enhanced colorectal cancer cells invasion, however, the mechanism remained unclear. Previous research on the association between inflammation and cancer mainly focused on tumor growth, angiogenesis, EMT, invasion, colonization and recruitment (2631). Besides, activation of several signal pathways was found to be involved in chronic inflammation such as NF-κB (32). Notably, recent studies revealed that activation of NF-κB increased glycolysis in the inflammatory environment (33). In the present study, we integrated the association between IL-8 and glycolysis. Inflammatory cytokine antibody array showed upregulation of IL-8 in LoVo-P cells, suggesting correlation between PRL-3 and IL-8. Furthermore, glycolysis of colorectal cancer cells was inhibited when IL-8 was neutralized, and inhibition was more significant in LoVo-P cells, indicating the important role of IL-8 in improving colorectal cancer cell glycolysis by PRL-3. Tumor metabolism reprogram available cell proliferation and even invasion since glycolysis produces more biological materials than oxidative phosphorylation (34). This study confirmed the function of glycolysis in colorectal cancer cell proliferation and invasion. Various research has explored the correlation between glycolysis and metastasis. For example, decreased pH may facilitate the invasion of tumor cells by promoting adjacent non-tumor cell apoptosis (35), and it has been found that by TGF-β dependent regulation of MMP2, lactate promotes tumor migration (36). Moreover, Glut1 was found to be correlated with MMP-2, which is important in degrading the basement membrane and improving cancer cell invasion (37); Hexokinase 2 was found to be a potent factor which is associated with cancer cell migration (17); PKM2 was found to promote cancer cell migration via activation of STAT signal pathway (18). In the present study, when lactate was neutralized or glycolysis related molecules and enzymes were inhibited, colorectal cancer cell invasion was repressed significantly, which is consistent with other research. Furthermore, we showed high expression of PRL-3, Glut1, HK2, PKM2, LDHA and IL-8 in tumor legion of colorectal cancer, and the positive correlation between PRL-3 and other molecules, indicating the possible clinical therapeutic strategies for colorectal cancer patients.

In summary, this study demonstrated that PRL-3 improved glycolysis of colorectal cancer cells via the secretion of IL-8. However, the detailed mechanism is still unknown and will be investigated in our following research.

Acknowledgments

The present study was supported by the National Natural Science Foundation of Guangdong Province (no. 2016A030313353), the National Natural Science Foundation of China (no. 81602539), the International Science and Technology Cooperation Program of Guangdong Province (no. 2013B051000025) and the Science and Technology Project of Guangdong Province (no. 2015A050502021).

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October-2017
Volume 51 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
x
Spandidos Publications style
Xu H, Zeng Y, Liu L, Gao Q, Jin S, Lan Q, Lai W, Luo X, Wu H, Huang Y, Huang Y, et al: PRL-3 improves colorectal cancer cell proliferation and invasion through IL-8 mediated glycolysis metabolism. Int J Oncol 51: 1271-1279, 2017
APA
Xu, H., Zeng, Y., Liu, L., Gao, Q., Jin, S., Lan, Q. ... Chu, Z. (2017). PRL-3 improves colorectal cancer cell proliferation and invasion through IL-8 mediated glycolysis metabolism. International Journal of Oncology, 51, 1271-1279. https://doi.org/10.3892/ijo.2017.4090
MLA
Xu, H., Zeng, Y., Liu, L., Gao, Q., Jin, S., Lan, Q., Lai, W., Luo, X., Wu, H., Huang, Y., Chu, Z."PRL-3 improves colorectal cancer cell proliferation and invasion through IL-8 mediated glycolysis metabolism". International Journal of Oncology 51.4 (2017): 1271-1279.
Chicago
Xu, H., Zeng, Y., Liu, L., Gao, Q., Jin, S., Lan, Q., Lai, W., Luo, X., Wu, H., Huang, Y., Chu, Z."PRL-3 improves colorectal cancer cell proliferation and invasion through IL-8 mediated glycolysis metabolism". International Journal of Oncology 51, no. 4 (2017): 1271-1279. https://doi.org/10.3892/ijo.2017.4090