Open Access

Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study

  • Authors:
    • Mayako Nishio-Nagai
    • Susumu Suzuki
    • Kazuhiro Yoshikawa
    • Ryuzo Ueda
    • Yoshiaki Kazaoka
  • View Affiliations

  • Published online on: October 2, 2017     https://doi.org/10.3892/ijo.2017.4142
  • Pages: 1471-1481
  • Copyright: © Nishio-Nagai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

FP treatment, which combines 5-fluorouracil (5-FU) and cisplatin (CDDP) chemotherapy, is widely used for treatment of advanced head and neck cancer (HNC). It has been suggested that these drugs cause immunomodulation in the cancer microenvironment, for example, downregulation of immunosuppressive cells such as regulatory T-cells (Tregs) and myeloid-derived suppressive cells (MDSCs), activating dendritic cells (DCs), and upregulation of tumor antigens and major histocompatibility complex (MHC) molecules in cancer cells leads to enhancement of cancer immunity, which is important in cancer treatment, as well as providing a direct killing effect. Therefore, development of chemoimmunotherapy by combining FP treatment with immunotherapy for HNC has become a recent challenging issue. However, the direct effects of these drugs on immune effector cells, especially cytotoxic T-lymphocytes (CTLs), are not well known. We have investigated the direct actions of these drugs on CTL functions in in vitro experiments using cytomegalovirus (CMV) pp65 antigen-specific CTLs (CMVpp65-CTLs) and oral squamous cell cancer (OSCC) cell lines overexpressing CMVpp65 antigen as target cells. Although CDDP partially inhibited proliferation of memory CMVpp65-CTL in peripheral blood, the proliferation was not inhibited by 5-FU. Cytotoxicity and the IFN-γ release response of the CMVpp65-CTLs were not inhibited by these drugs, and it is important to note that these drugs, especially 5-FU, sensitized OSCC cell lines to CMVpp65-CTL. Furthermore, CMVpp65-CTL cytotoxicity to CDDP-resistant OSCC cells, HSC-3/CDDP-R1, was the same as the cytotoxicity to the parental cells. Thus, we suggest that combined immunotherapy with FP treatment is an effective novel HNC treatment.
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November-2017
Volume 51 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Nishio-Nagai M, Suzuki S, Yoshikawa K, Ueda R and Kazaoka Y: Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study. Int J Oncol 51: 1471-1481, 2017
APA
Nishio-Nagai, M., Suzuki, S., Yoshikawa, K., Ueda, R., & Kazaoka, Y. (2017). Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study. International Journal of Oncology, 51, 1471-1481. https://doi.org/10.3892/ijo.2017.4142
MLA
Nishio-Nagai, M., Suzuki, S., Yoshikawa, K., Ueda, R., Kazaoka, Y."Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study". International Journal of Oncology 51.5 (2017): 1471-1481.
Chicago
Nishio-Nagai, M., Suzuki, S., Yoshikawa, K., Ueda, R., Kazaoka, Y."Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study". International Journal of Oncology 51, no. 5 (2017): 1471-1481. https://doi.org/10.3892/ijo.2017.4142