Open Access

MicroRNA expression profiles in non‑epithelial ovarian tumors

  • Authors:
    • Roger K. Chang
    • Xidan Li
    • Ninni Mu
    • Olga Hrydziuszko
    • Beatriz Garcia-Majano
    • Catharina Larsson
    • Weng-Onn Lui
  • View Affiliations

  • Published online on: November 10, 2017     https://doi.org/10.3892/ijo.2017.4200
  • Pages:55-66
  • Copyright: © Chang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views
0

Abstract

Ovarian germ cell tumors (OGCTs) and sex cord stromal tumors (SCSTs) are rare gynecologic tumors that are derived from germ and stromal cells, respectively. Unlike their epithelial counterparts, molecular pathogenesis of these tumor types is still poorly understood. Here, we characterized microRNA (miRNA) expression profiles of 9 OGCTs (2 malignant and 7 benign) and 3 SCSTs using small RNA sequencing. We observed significant miRNA expression variations among the three tumor groups. To further demonstrate the biological relevance of our findings, we selected 12 miRNAs for validation in an extended cohort of 16 OGCTs (9 benign and 7 malignant) and 7 SCSTs by reverse transcription-quantitative polymerase chain reaction. Higher expression of miR‑373‑3p, miR‑372‑3p and miR‑302c‑3p and lower expression of miR‑199a‑5p, miR‑214‑5p and miR‑202‑3p were reproducibly observed in malignant OGCTs as compared to benign OGCTs or SCSTs. Comparing with benign OGCTs, miR‑202c‑3p and miR‑513c‑5p were more abundant in SCSTs. Additionally, we examined Beclin 1 (BECN1), a target of miR‑199a‑5p, in the clinical samples using western blot analysis. Our results show that BECN1 expression was higher in malignant OGCTs than benign OGCTs, which is concordant with their lower miR‑199a‑5p expression. This study suggests that these miRNAs may have potential value as tumor markers and implications for further understanding the molecular basis of these tumor types.

Related Articles

Journal Cover

January 2018
Volume 52 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

2016 Impact Factor: 3.079
Ranked #33/217 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Chang, R.K., Li, X., Mu, N., Hrydziuszko, O., Garcia-Majano, B., Larsson, C., & Lui, W. (2018). MicroRNA expression profiles in non‑epithelial ovarian tumors. International Journal of Oncology, 52, 55-66. https://doi.org/10.3892/ijo.2017.4200
MLA
Chang, R. K., Li, X., Mu, N., Hrydziuszko, O., Garcia-Majano, B., Larsson, C., Lui, W."MicroRNA expression profiles in non‑epithelial ovarian tumors". International Journal of Oncology 52.1 (2018): 55-66.
Chicago
Chang, R. K., Li, X., Mu, N., Hrydziuszko, O., Garcia-Majano, B., Larsson, C., Lui, W."MicroRNA expression profiles in non‑epithelial ovarian tumors". International Journal of Oncology 52, no. 1 (2018): 55-66. https://doi.org/10.3892/ijo.2017.4200