miR‑149‑5p promotes chemotherapeutic resistance in ovarian cancer via the inactivation of the Hippo signaling pathway
- Authors:
- Published online on: January 24, 2018 https://doi.org/10.3892/ijo.2018.4252
- Pages: 815-827
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Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Ovarian cancer is one of the most common types of gynecological cancers and the 5th leading cause of cancer-related mortality in females (1,2). Despite enormous improvements over the past years, the prognosis of patients with ovarian cancer remains dismal, and this is largely attributed to chemotherapeutic resistance following several cycles of treatment. Hence, a better understanding of the underlying mechanisms responsible for chemotherapeutic resistance in ovarian cancer may aid in the development of novel targets for therapeutic intervention in ovarian cancer.
The Hippo pathway represents a novel tumor suppressor pathway and the dysregulation of Hippo signaling has been demonstrated to be involved in several biological process and tumor development (3–5). From a molecular perspective, 4 core kinase components have been reported to be essential for the tumor suppressive function of Hippo signaling, including mammalian STE20-like kinase (MST)1/2, protein salvador homolog 1 (SAV1), large tumor-suppressor kinase (LATS)1/2 and Mps one binder kinase activator-like 1 (MOB1) (5). Physiologically, the Hippo signaling pathway is active, which tightly controls the activity of 2 critical downstream effectors of the Hippo pathway, YAP and tafazzin (TAZ), both spatially and temporally via the phosphorylation of YAP and TAZ by these 4 core kinase cassettes, leading to the degradation of YAP and TAZ (6,7). Conversely, when the activity of Hippo signaling is inhibited by unknown mechanisms, phosphorylated YAP and TAZ translocate to the nucleus and elicit the transcriptional activity of TEA domain (TEAD) family members, including TEAD1-4 (8–10), which further transcriptionally activates downstream effectors to exert its versatile functions (11–13). Recently, accumulating evidence has indicated that the inactivation of Hippo promotes the development of chemotherapeutic resistance in numerous human cancers. For example, the study by Lai et al reported that upregulation of TAZ induced Taxol resistance in breast cancer cells via transcriptionally activating Cyr61 and CTGF (14); moreover, the hyperactivation of YAP has been reported to be positively associated with a poor chemotherapeutic response to taxanes and a poor prognosis in patients with ovarian cancer (15), indicating that the inactivation of the Hippo pathway is crucial for the development of cancer chemoresistance. Therefore, a better understanding of the mechanisms responsible for the inactivation of the Hippo pathway may provide new insight and may aid in the development of more effective treatment strategies for ovarian cancer.
MicroRNAs (miRNAs or miRs) are small endogenous non-coding RNAs that are able to repress a variety of target genes at the post-transcriptional level by binding with the 3′ untranslated region (3′UTR) of the target gene mRNAs (16). Numerous studies have reported that miRNAs play crucial roles in tumorigenesis, malignant progression and the metastasis of cancers through various mechanisms (17–22). Furthermore, studies have also supported that the up- or downregulation of a certain miRNA can be directly tied to the response to chemotherapeutic agents (23–26). In this study, from a publically available miRNA dataset from The Cancer Genome Atlas (TCGA) and our experiments, we found that miR-149-5p expression was significantly elevated in chemo-resistant ovarian cancer tissues and cell lines compared with chemosensitive ovarian cancer ones. Furthermore, the silencing of miR-149-5p increased the apoptotic ratio and decreased the mitochondrial potential of ovarian cancer cells in response to cisplatin (CDDP) in vitro, and also enhanced the chemotherapeutic sensitivity of ovarian cancer cells to CDDP in vivo. Conversely, the upregulation of miR-149-5p decreased the apoptotic ratio and increased the mitochondrial potential of ovarian cancer cells in response to to CDDP in vivo. Our results further demonstrated that miR-149-5p directly targeted MST1 and SAV1, leading to the inactivation of TEAD transcription. Importantly, the effects of miR-149-5p silencing on the apoptotic ratio, mitochondrial potential and caspase-3 activity in ovarian cancer cells were attenuated by the individual knockdown of MST1 and SAV1. Therefore, our findings reveal a novel mechanism through which miR-149-5p induces chemotherapeutic resistance in ovarian cancer, suggesting that miR-149-5p may be used as a chemotherapeutic response indicator in ovarian cancer.
Materials and methods
Cell culture and agents
The ovarian cancer cell lines, TOV-21G, A2780, OVCAR-3, Caov-3, ES-2, HO-8910 and SK-OV-3, were obtained from the Shanghai Chinese Academy of Sciences cell bank (Shanghai, China), and all human ovarian cancer cell lines were maintained in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS (HyClone, Logan, UT, USA). CDDP (Cat. no. P4394; Sigma-Aldrich, St. Louis, MO, USA), which undergoes relatively slow solvolysis in DMSO, was dissolved in 0.9% NaCl at 4 mM, immediately aliquoted and stored at −70°C. Thawed aliquots were used only once. The stock solutions were further diluted with DMSO and added to the cell cultures with the final DMSO concentrations being <0.05%. The ovarian cancer cells were treated with cisplatin (5 µM, 48 h) before further functional assays.
Patients and tumor tissues
A total of 20 ovarian cancer tissues and 10 benign ovarian lesion tissues, including 4 simple ovarian cyst and 6 simple ovarian serous cystadenoma were obtained during surgery at the Sun Yat-sen University Cancer Center (Guangzhou, China) (Tables I and II). Patients were diagnosed based on clinical and pathological evidence, and the specimens were immediately snap-frozen and stored in liquid nitrogen tanks. For the use of these clinical samples for research purposes, prior patient consents and approval from the Institutional Research Ethics Committee of Sun Yat-sen University Cancer Center were obtained (ethics approval no. 308-2016-03-01).
 | Table IThe basic information of 20 patients with ovarian serous cystadenocarcinoma for miR-149-5p expression analysis. |
| Table IIThe basic information of 10 patients with benign ovarian lesions for miR-149-5p expression analysis. |
RNA extraction, reverse transcription and real-time PCR
Total RNA from the tissues or cells was extracted using TRIzol reagent (Life Technologies/Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's instructions. Messenger RNAs (mRNAs) were polyadenylated using a poly-A polymerase-based First-Strand Synthesis kit (Takara, Dalian, China) and the reverse transcription (RT) from total mRNA was performed using a PrimeScript RT Reagent kit (Takara) according to the manufacturer's instructions. miRNAs were reverse transcribed from total mRNA using the RevertAid First Strand cDNA Synthesis kit (Thermo Fisher Scientific) according to the manufacturer's instructions. Complementary DNA (cDNA) was amplified and quantified on an ABI 7500HT system (Applied Biosystems, Foster City, CA, USA) using SYBR-Green I (Applied Biosystems). The RT-PCR reaction was performed with the following parameter values: 15 min at 37°C, 10 min at 65°C, 5 min at 85°C and −20°C until use. RT-PCR analysis was performed on an iQ5 Real Time PCR Detection System (Bio-Rad, Hercules, CA, USA) with a 20 µl volume reaction containing 2 µl reverse transcription product, 10 µl 2All-in-One™ Q-PCR Mix, 2 µl PCR Forward Primer (2 µM), 2 µl Universal Adaptor PCR Primer (2 µM) and 4 µl ddH2O. The reactions were incubated in 96-well plates at 95°C for 10 min, following by 40 cycles, and then ramped from 66°C to 95°C to obtain the melting curve. Each sample was analyzed in triplicate. The primers sequence and the product size were listed in Table III. Real-time PCR was performed according to a standard method, as previously described (27). Primers for U6 and miR-149-5p (miRQ0000450-1-1) were synthesized and purified by RiboBio (Guangzhou, China). U6 or glycer-aldehyde-3-phosphate dehydrogenase (GAPDH) was used as endogenous controls for miRNA or mRNA respectively. Relative fold expression was calculated with the comparative threshold cycle (2−ΔΔCq) method as previously described (28).
Plasmids, small interfering RNA and transfection
The miR-149-5p expression plasmid was generated by cloning the genomic pre-miR-149-5p gene into the retroviral transfer plasmid pMSCV-puro (Clontech Laboratories Inc., Tokyo, Japan) to generate plasmid pMSCV-miR-149-5p. Subsequently, pMSCV-miR-149-5p was co-transfected with the pIK packaging plasmid (Cat. no. 75483; ATCC, VA, USA) into 293FT (CRL-1573) cells using the standard calcium phosphate transfection method, as previously described (29). At 36 h after the co-transfection, the supernatants were collected and incubated with the cells to be infected for 24 h in the presence of polybrene (2.5 µg/ml). Following infection, puromycin (1.5 µg/ml) was used to select stably transduced cells over a 10-day period. The luciferase reporter system of pTEAD-luc (Cat. no. 83467; Addgene, Cambridge, MA, USA) was used to examine the transcriptional activity of TEAD. The 3′-untranslated region (3′UTR) regions of human MST1 and SAV1 were PCR-amplified from genomic DNA and cloned into the pmirGLO luciferase reporter vector (Promega, Madison, WI, USA) and the list of primers used in clone reactions are presented in Table IV. The miArrest plasmids for anti-miR-149-5p and negative control plasmids were constructed and cloned into phU6 plasmids by GeneChem (Shanghai, China). The sequence of anti-miR-149-5p was GGGAGTGAAGACACGGAGCCAGA. Small interfering RNA (siRNA) for YAP, TAZ, MST1 and SAV1 knockdown were obtained from Ribobio. The transfection of siRNAs and the plasmids was performed using Lipofectamine 3000 (Life Technologies/Thermo Fisher Scientific) according to the manufacturer's instructions.
Western blot analysis
Nuclear/cytoplasmic fractionation was carried out using the Cell Fractionation kit (Cell Signaling Technology, Danvers, MA, USA) according to the manufacturer's instructions, and the whole cell lysates were extracted using RIPA Buffer (Cell Signaling Technology). Western blot analysis was performed according to a standard method, as previously described (30). Antibodies against YAP (Cat. no. ab81183), TAZ (Cat. no. ab93362), MST1 (Cat. no ab76822), SAV1 (Cat. no. ab105105), Bcl-2 (Cat. no. ab194583) and Bcl-xL (Cat. no. ab32370) were purchased from Abcam (Cambridge, MA, USA), and antibodies against caspase-3 (Cat. no. 9662) and caspase-9 (Cat. no. 9501) were from Cell Signaling Technology. The membranes were stripped and reprobed with an anti-α-tubulin antibody (Sigma-Aldrich) as the loading control.
Flow cytometric analysis
Flow cytometric analysis of apoptosis was carried out using the FITC Annexin V Apoptosis Detection kit I (BD Biosciences, San Jose, CA, USA), and was presented as the protocol described below. Briefly, the cells were dissociated with trypsin and resuspended at 1×106 cells/ml in binding buffer with 50 µl/ml FITC Annexin V and 50 µl/ml PI. The cells were subsequently incubated for 15 min at room temperature, and were then were analyzed using a Gallios flow cytometer (Beckman Coulter, Atlanta, GA, USA). The cell inner mitochondrial membrane potential (Δψm) was detected by flow cytometry using the MitoScreen JC-1 staining kit (BD Biosciences), and was presented as the protocol described below. Briefly, the cells were dissociated with trypsin and resuspended at 1×106 cells/ml in Assay Buffer, and then incubated at 37°C for 15 min with 10 µl/ml JC-1. Before analyzed by flow cytometer, cells were washed twice by Assay Buffer. Flow cytometry data were analyzed using FlowJo 7.6 software (TreeStar Inc., Ashland, OR, USA).
Caspase-9 or caspase-3 activity assays
The activity of caspase-9 or caspase-3 was analyzed by spectrophotometry using the Caspase-9 Colorimetric Assay kit or Caspase-3 Colorimetric Assay kit (KeyGen BioTech, Nanjing, China), and was presented as the protocol described below. Briefly, 5×106 cells or 100 mg fresh tumor tissues were washed with cold PBS and re-suspended in lysis buffer and incubated on ice for 30 min. Subsequently, 50 µl cell suspension was mixed with 50 µl reaction buffer, and 5 µl caspase-3/-9 substrate, followed by incubation at 37°C for 4 h. The absorbance was measured at 405 nm, and BCA protein quantitative analysis was used as the reference to normalize each experimental group.
Establishment of tumor xenograft model
For the establishment of a tumor xenograft model, 20 BALB/c-nu mice (6 weeks old, weighing 10–12 g) were purchased from Hunan Silaikejingda Laboratory Animal Technology Co. (Hunan, China) and randomly divided into 4 groups (n=5 per group) and the indicated cells (1×106) were inoculated subcutaneously into the inguinal folds of the nude mice. At 7 days after the cell inoculation, the mice were injected intraperitoneally 15 mg/kg CDDP twice per week for 5 weeks in one group of mice injected with the vector cells and one group of mice injected with the anti-miR-149-5p cells respectively. Tumor volume was determined using an external caliper and calculated using the equation (L × W2)/2. On day 40, the animals were euthanized, and the tumors were excised, weighed and stored in liquid nitrogen tanks. For survival analyses, the mice were monitored daily for signs of discomfort, and were either euthanized all at one time or individually when presenting signs of distress, such as a 10% loss of body weight, or head tilting. Overall survival was assessed by the Kaplan-Meier method, using an exact log-rank test. Ethics approval for the animal experiments was provided by The Institutional Animal Care and Use Committee of Sun Yat-sen University Cancer Center (ethics approval no. 102012016008I).
Luciferase assay
The cells (4×104) were seeded in triplicate in 24-well plates and luciferase assay was carried out as previously described (31). The cells were transfected with 100 ng of HOP-flash reporter luciferase plasmid, HIP-flash (HOP-flash mutant), pmirGLO-MST1-3′UTR or -SAV1-3′UTR, luciferase plasmid, plus 5 ng pRL-TK Renilla plasmid (Promega) using Lipofectamine 3000 (Invitrogen) according to the manufacturer's instructions. Luciferase and Renilla signals were measured at 36 h following transfection using a Dual Luciferase Reporter Assay kit (Promega) according to the manufacturer's instructions.
miRNA immunoprecipitation
The cells were co-transfected with the HA-Ago2 plasmid (10822; Addgene), followed by HA-Ago2 immunoprecipitation using HA-antibody (Cat. no. H3663, Sigma-Aldrich) as previously described (32). Real-time PCR analysis of the IP material was used to test the association of the mRNA of YAP1 and TAZ with the RISC complex.
Gene set enrichment analysis (GSEA)
The miRNA dataset of ovarian cancer from TCGA was downloaded and we then procured the expression value of the corresponding genes from the Level 3 data of each sample (the unit was RNA-Seq by expectation maximization, RKPM). We then analyzed the log2 value of each sample using Excel 2010 and GraphPad 5 software, as well as statistically analyzed the miRNA expression levels of all ovarian cancer tissues using a paired t-test or unpaired t-test. GSEA was performed with RNAseqV2 dataset of ovarian cancer from TCGA as the MSigDB dataset. The high and low expression level of miR-149-5p was stratified by the medium expression level of miR-149-5p in ovarian cancer tissues. Gene set analysis was performed by Molecular Signatures Database version 5.2.
Identification of potential targets of miR-149-5p
As previously described (33,34), the TargetScan (http://www.targetscan.org/vert_71/) and miRanda (http://34.236.212.39/microrna/home.do) databases were used to identify possible targets of miR-149-5p.
Statistical analysis
All values are presented as the means ± standard deviation (SD). Significant differences were determined using GraphPad 5.0 software (GraphPad Software, Inc., La Jolla, CA, USA). One-way ANOVA with Tukey's post hoc test was used to determine statistical differences between multiple groups. An unpaired or paired t-test was used to determine statistical differences between 2 groups and confidence intervals, 95%. A value of p<0.05 was considered to indicate a statistically significant difference. All the experiments were repeated 3 times.
Results
miR-149-5p is upregulated in chemoresistant ovarian cancer tissues
By analyzing TCGA ovarian cancer miRNA sequencing datasets, we found that miR-149-5p expression was elevated in chemoresistant ovarian cancer tissues compared with chemosensitive ovarian cancer tissues (Fig. 1A). We further examined miR-149-5p expression in our own 20 ovarian cancer tissues, including 10 complete response, 1 partial response, 3 stable disease and 6 progressive disease ovarian cancer tissues perspectively. Consistently, we found that miR-149-5p expression in the ovarian cancer tissues from patients with stable and progressive disease was higher than that in ovarian cancer tissues from patients with complete response (Fig. 1B). Furthermore, miR-149-5p expression was markedly upregulated in ovarian cancer tissues compared with benign ovarian disease tissues (Fig. 1C). The expression levels of miR-149-5p in the ovarian cancer cell lines were evaluated and the results revealed that compared with the chemosensitive ovarian cancer cell lines (TOV-21G, A2780, OVCAR-3 and Caov-3), miR-149-5p expression was differentially upregulated in the chemoresistant ovarian cancer cell lines (ES-2, HO-8910, SK-OV-3) (Fig. 1C). Thus, these results suggest that a high expression of miR-149-5p positively correlates with a poor chemotherapeutic response in patients with ovarian cancer.
Upregulation of miR-149-5p enhances the chemoresistance of ovarian cancer cells to CDDP
The role of miR-149-5p in the chemoresistance of ovarian cancer was further examined. Based on the results of the expression level of miR-149-5p shown in Fig. 1C, we decided to exogenously overexpress miR-149-5p via viral transduction in the chemosensitive TOV-21G and A2780 cells (Fig. 2A). As shown in Fig. 2B, miR-149-5p overexpression decreased the apoptotic rates of the TOV-21G and A2780 cells treated with CDDP. The effects of miR-149-5p overexpression on mitochondrial potential were further investigated and the results revealed that miR-149-5p overexpression increased the mitochondrial potential of the TOV-21G and A2780 cells treated with CDDP (Fig. 2C). The effects of the upregulation of miR-149-5p on apoptosis and caspase protein expression were further confirmed by examining the activity of caspase-9 and caspase-3, as well as the expression levels of the anti-apoptotic proteins, Bcl-2 and Bcl-xL. The results revealed that the overexpression of miR-149-5p reduced the activity and expression of caspase-3 and caspase-9, and increased the expression Bcl-2 and Bcl-xL (Fig. 2D–G). Therefore, these results demonstrate that miR-149-5p promotes the resistance of ovarian cancer cells to CDDP in vivo.
Silencing of miR-149-5p sensitizes ovarian cancer cells to CDDP
We further endogenously silenced miR-149-5p by transfecting anti-miR-149-5p into the chemoresistant HO-8910 and SK-OV-3 cells (Fig. 3A) due to the higher expression levels of miR-149-5p in the HO-8910 and SK-OV-3 cells (as shown in Fig. 1D). As shown in Fig. 3B, the silencing of miR-149-5p markedly increased the apoptotic rate of the HO-8910 and SK-OV-3 cells treated with CDDP. Furthermore, the silencing of miR-149-5p decreased the mitochondrial potential of the HO-8910 and SK-OV-3 cells treated with CDDP (Fig. 3C). In addition, the silencing of miR-149-5p enhanced the activity and expression of caspase-3 and caspase-9, and decreased the expression levels of Bcl-2 and Bcl-xL (Fig. 3D–G). Collectively, these results demonstrate that the silencing of miR-149-5p promotes the resistance of ovarian cancer cells to CDDP.
Silencing of miR-149-5p enhances the chemosensitivity of ovarian cancer cells to CDDP in vivo
We further examined the effects of miR-149-5p on the chemoresistance of ovarian cancer cells in vivo. Mice were randomly divided into 4 groups (n=5/group). The vector cells were inoculated subcutaneously into 2 groups of mice in the left dorsal flank, and the other 2 groups of mice were inoculated with anti-miR-149-5p cells (1×106 SK-OV-3 cells per mouse). After 7 days, one group of mice injected with the vector cells and one group of mice injected with the anti-miR-149-5p cells were intraperitoneally injected with CDDP twice per week for 5 weeks respectively [15 mg/kg (Fig. 4A)]. As shown in Fig. 4B and C, the tumor volume and tumor weight were differentially decreased in the anti-miR-149-5p group, CDDP treatment alone group and anti-miR-149-5p plus CDDP group compared to the controls, particularly in the anti-miR-149-5p plus CDDP group. Moreover, the mice injected with anti-miR-149-5p-transfected cells in combination with CDDP displayed a longer survival time (Fig. 4D). Caspase-3 and caspase-9 activities were markedly increased in the mice injected with anti-miR-149-5p-transfected cells plus CDDP (Fig. 4E). Taken together, these findings demonstrated that the silencing of miR-149-5p sensitized ovarian cancer cells to CDDP in vivo.
miR-149-5p inactivates the Hippo signaling pathway
To investigate the mechanisms underlying the promoting effect of miR-149-5p on chemoresistance in ovarian cancer, we performed a Gene set enrichment analysis (GSEA) of miR-149-5p expression against the oncogenic signatures collection of the MSigDB and found that miR-149-5p overexpression positively correlated with the YAP and TAZ stimulated signatures (35) (Fig. 5A), suggesting that miR-149-5p regulates the Hippo signaling pathway, which have been reported to play important roles in the development of cancer chemoresistance (14,15). As shown in Fig. 5B, we found that the upregulation of miR-149-5p increased, while the silencing of miR-149-5p decreased TEAD-dependent luciferase activity. Moreover, the results of cellular fractionation and western blot analysis revealed that the overexpression of miR-149-5p enhanced the nuclear accumulation of YAP and TAZ, while the silencing of miR-149-5p suppressed their nuclear expression (Fig. 5C). Real-time PCR analysis also revealed that miR-149-5p overexpression significantly increased, while the silencing of miR-149-5p inhibited the expression levels of multiple downstream genes, including connective tissue growth factor (CTGF), cysteine rich angiogenic inducer 61 (CYR61), homeobox A1 (HOXA1) and SRY-box 9 (SOX9) in ovarian cancer cells (Fig. 5D). Thus, these results demonstrate that the downregulation of miR-149-5p inactivates the Hippo signaling pathway in ovarian cancer cells.
YAP and TZA are essential for miR-149-5p upregulation-induced chemoresistance in ovarian cancer cells
We then explored the functional significance of YAP and TAZ in the chemoresistance of ovarian cancer cells. As shown in Fig. 5E, the individual inhibition of YAP or TAZ in miR-149-5p-overex-pressing cells abrogated the stimulatory effects of miR-149-5p overexpression on mitochondrial potential. Conversely, the inhibitory effects of miR-149-5p upregulation on the apoptotic ratio, and caspase-3 and caspase-9 activities were reversed by the individual silencing of YAP and TAZ in ovarian cancer cells (Fig. 5F–H). Taken together, our results indicate that YAP and TAZ are functionally relevant effectors of miR-149-5p in the chemotherapeutic response of ovarian cancer cells.
MST1 and SAV1 are authentic targets of miR-149-5p in ovarian cancer cells
Using the publically available algorithms, TargetScan and miRanda, we found that kinase components of Hippo signaling, including MST1 and SAV1, may be potential targets of miR-149-5p (Fig. 6A). Western blot analysis revealed that miR-149-5p overexpression decreased the expression levels of MST1 and SAV1; conversely, the silencing of miR-149-5p increased their expression levels in the ovarian cancer cells (Fig. 6B). Furthermore, luciferase assay revealed that the upregulation of miR-149-5p suppressed, while the silencing of miR-149-5p enhanced the reporter activity driven by the 3′UTRs of the MST1 and SAV1 transcripts, but not by the mutant 3′UTRs of these transcripts within miR-149-5p-binding seed regions in ovarian cancer cells (Fig. 6C and D). Moreover, microribonucleoprotein (miRNP) immunoprecipitation (IP) assay revealed an association of miR-149-5p with MST1 and SAV1 transcripts (Fig. 6E and F), further indicating the direct repressive effects of miR-149-5p on MST1 and SAV1. Importantly, the stimulatory effects of anti-miR-149-5p on the apoptotic ratio, and caspase-3 and caspase-9 activities were abrogated by the individual silencing of MST1 and SAV1; however, the inhibitory effects of miR-149-5p silencing were reversed by the individual silencing of MST1 and SAV1 in ovarian cancer cells (Fig. 6G–J). Collectively, our results indicate that miR-149-5p promotes the chemoresistance of ovarian cancer cells by directly targeting MST1 and SAV1, leading to the inactivation of Hippo signaling.
Discussion
In the present study, our results revealed that miR-149-5p expression was upregulated in chemoresistant ovarian cancer tissues and cell lines compared with chemosensitive ovarian cancer tissues and cell lines. The upregulation of miR-149-5p enhanced, while the silencing of miR-149-5p inhibited the chemoresistance of ovarian cancer cells to CDDP in vivo and in vivo. Our results further revealed that miR-149-5p directly suppressed MST1 and SAV1 expression, leading to the inactivation of TEAD transcription and the downregulation of Hippo downstream target genes. Therefore, our findings reveal a novel biological role of miR-149-5p in the chemotherapeutic resistance of ovarian cancer to CDDP.
The dysregulation of the Hippo signaling pathway has been demonstrated to play an important role in the chemotherapeutic resistance of cancer. YAP and TAZ are important downstream effectors of the Hippo pathway and are suppressed by active Hippo signaling (36,37). Studies have shown that the upregulation of YAP and TAZ positively correlates with the chemoresistance of several human cancer types (38,39). Furthermore, the downregulation of the Hippo pathway core kinase components has been reported to be implicated in chemotherapeutic resistance of cancer cells to diverse chemotherapeutic drugs. For example, the downregulation of MST1, which is mediated by heat shock protein 70 (Hsp70) in a proteasomal-dependent manner, promotes the CDDP resistance of prostate cancer cells (40). Additionally, the overexpression of miR-181c contributes to chemoresistance in pancreatic cancer by directly targeting MST1 and SAV1 (41). These studies indicate that the inactivation of the Hippo signaling pathway, either the upregulation of the downstream effectors, YAP and TAZ, or the downregulation of core components of the Hippo signaling pathway, confers chemotherapeutic resistance in human cancers. In this study, our results demonstrated that a high expression of miR-149-5p inactivated Hippo signaling by directly suppressing MST1 and SAV1, which further promoted the chemotherapeutic resistance of ovarian cancer cells to CDDP. Importantly, the inhibitory effects of anti-miR-149-5p on the chemoresistance of ovarian cancer cells to CDDP were attenuated by the individual knockdown of MST1 and SAV1. There finding indicate that miR-149-5p renders CDDP chemoresistance in ovarian cancer cells by targeting MST1 and SAV1, leading to the inactivation of Hippo signaling pathway.
The inactivation of Hippo signaling has been extensively reported to be implicated in the initiation and progression of ovarian cancer through various mechanisms. Fan et al have reported that the disruption of Hippo signaling by Sphingosine 1-phosphate (S1P) contributed to the development of ovarian cancer by promoting unlimited cell proliferation (42). YAP, a major effector of the Hippo signaling pathway, promotes the initiation and progression of ovarian cancer by forming autocrine loops with ERBB signaling (43). Furthermore, YAP confers the resistance of ovarian cancer cells to chemotherapeutic agents, and a high nuclear YAP expression strongly correlates with a poor prognosis in patients with ovarian cancer (44,45). These findings indicate that the disruption of Hippo signaling plays an oncogenic role in ovarian cancer. In this study, our results revealed that miR-149-5p inactivated Hippo signaling by targeting MST1 and SAV1, which further rendered chemotherapeutic resistance in ovarian cancer. Thus, our results present a novel role of miR-149-5p in promoting the chemoresistance of ovarian cancer cells to CDDP via the direct suppression of MST1 and SAV1, leading to the inactivation of Hippo signaling.
There is evidence to indicate that miRNAs plays an important role in regulating the activation of Hippo signaling via the post-transcriptional suppression of multiple target mRNAs of Hippo signaling components (46,47). For example, miR-129-5p has been shown to inhibit proliferation and survival by targeting the downstream co-activators, YAP and TAZ, of Hippo signaling in ovarian cancer (48); moreover, the upregulation of miR-130b has been shown to promote cancer stem cell characteristics in glioblastoma cells by directly suppressing MST1 and SAV1 (49). These studies demonstrate that the aberrant expression of miRNAs is involved in the development and progression of cancer via the Hippo signaling pathway. In this study, through bioinformatics analysis, we found that the core kinase components of Hippo signaling are potential targets of miR-149-5p. Furthermore, the upregulation of miR-149-5p enhanced the tanscriptional activity of TEAD, nuclear translocation of TAP and TAZ and expression levels of multiple downstream genes of Hippo signaling pathway; conversely, silencing miR-149-5p yielded an opposite effect. Collectively, our results uncover a novel mechanism of the inactivation of the Hippo signaling pathway, which further promotes the chemoresistance of ovarian cells to CDDP.
The upregulation of miR-149-5p has been reported to be implicated in the progression and metastasis of several malignancies, including non-small cell lung cancer, squamous cell carcinomas of the nasopharynx and prostate cancer, and a high expression of miR-149-5p predicts a poor prognosis (50–52). Furthermore, other studies have indicated that miR-149-5p expression is decreased in hepatocellular carcinoma, breast cancer and colorectal cancer (53–55). These studies suggested that the pro- or anticancer roles of miR-149-5p were tumor-type dependent. However, little is known about the biological roles of miR-149-5p in ovarian cancer. In this study, we found that miR-149-5p was markedly upregulated in chemoresistant ovarian cancer tissues and cell lines compared with chemo-sensitive ovarian cancer tissues and cell lines. Moreover, the upregulation of miR-149-5p enhanced, while the silencing of miR-149-5p inhibited the chemoresistance of ovarian cancer cells to CDDP in vivo and in vivo. Our results further revealed that miR-149-5p directly suppressed MST1 and SAV1 expression, leading to the inactivation of TEAD transcription and the downregulation of nuclear YAP and TAZ and Hippo signaling downstream target genes. Therefore, our results demonstrate that miR-149-5p promotes the chemoresistance of ovarian cancer cells to CDDP by inactivating the Hippo signaling pathway.
In conclusion, our study reveals that the upregulation of miR-149-5p contributes to the chemoresistance of ovarian cancer cells to CDDP as a pivotal suppressor of Hippo signaling pathway. Thus, an in-depth understanding of the precise role of miR-149-5p in the chemoresistance of ovarian cancer faciliates to increase our knowledge of the development of chemoresistance in ovarian cancer, which may aid in the development of noveltherapeutic strategies against ovarian cancer.
Acknowledgments
This study was supported by grants from the National Natural Science Foundation of China (no. 81672561).
Notes
[1] Competing interests
The authors declare that they have no competing interests.
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