Knockdown of microRNA‑29a regulates the expression of apoptosis‑related genes in MCF‑7 breast carcinoma cells

  • Authors:
    • Gholamreza Khamisipour
    • Elham Mansourabadi
    • Behrouz Naeimi
    • Ali Moazzeni
    • Rahim Tahmasebi
    • Mojtaba Hasanpour
    • Majid Mosahebi Mohammadi
    • Zahra Mansourabadi
    • Shakib Shamsian
  • View Affiliations

  • Published online on: December 8, 2017     https://doi.org/10.3892/mco.2017.1528
  • Pages:362-369
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Abstract

MicroRNA (miR), as non-coding small RNA, are key regulators of cancer‑related biological cell processes and contribute to tumor growth through regulation of groups of pro‑ and anti‑apoptotic genes. The present study aimed to investigate the effects of miR‑29a on the expression of genes involved in apoptosis, including p21, B‑cell lymphoma 2 (BCL‑2), p53 and survivin. The MCF‑7 breast cancer cell line was transfected with anti‑miR‑29a and treated with Taxol in subdivided treatment groups including: Scramble; anti‑miR‑29a; anti‑miR‑29a + Taxol; Taxol; and control. Expression levels of p21, BCL‑2, p53 and survivin were evaluated using reverse transcription‑quantitative polymerase chain reaction. miR‑29a knockdown resulted in p21 and p53 upregulation and a decrease in survivin expression. These results indicated that miR‑29a inhibition regulates apoptosis. The present data suggested that miR‑29a inhibition may be a promising strategy for the induction of apoptosis of tumor cells.

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February 2018
Volume 8 Issue 2

Print ISSN: 2049-9450
Online ISSN:2049-9469

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APA
Khamisipour, G., Mansourabadi, E., Naeimi, B., Moazzeni, A., Tahmasebi, R., Hasanpour, M. ... Shamsian, S. (2018). Knockdown of microRNA‑29a regulates the expression of apoptosis‑related genes in MCF‑7 breast carcinoma cells. Molecular and Clinical Oncology, 8, 362-369. https://doi.org/10.3892/mco.2017.1528
MLA
Khamisipour, G., Mansourabadi, E., Naeimi, B., Moazzeni, A., Tahmasebi, R., Hasanpour, M., Mosahebi Mohammadi, M., Mansourabadi, Z., Shamsian, S."Knockdown of microRNA‑29a regulates the expression of apoptosis‑related genes in MCF‑7 breast carcinoma cells". Molecular and Clinical Oncology 8.2 (2018): 362-369.
Chicago
Khamisipour, G., Mansourabadi, E., Naeimi, B., Moazzeni, A., Tahmasebi, R., Hasanpour, M., Mosahebi Mohammadi, M., Mansourabadi, Z., Shamsian, S."Knockdown of microRNA‑29a regulates the expression of apoptosis‑related genes in MCF‑7 breast carcinoma cells". Molecular and Clinical Oncology 8, no. 2 (2018): 362-369. https://doi.org/10.3892/mco.2017.1528