MAGE-A9 in head and neck cancer: Prognostic value and preclinical findings in the context of irradiation

  • Authors:
    • Till J. Meyer
    • Stefan Hartmann
    • Gisela Wohlleben
    • Muna Brisam
    • Axel Seher
    • Alexander C. Kübler
    • Bülent Polat
    • Urs D.A. Müller‑Richter
  • View Affiliations

  • Published online on: January 19, 2018     https://doi.org/10.3892/mco.2018.1558
  • Pages: 513-519
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Abstract

Radiotherapy alone, or as an addition to surgery is important for the treatment of head and neck squamous cell carcinoma (HNSCC). In addition to their expression in germ cells, melanoma associated antigens‑A (MAGE‑A) are only expressed in malignant tissue. Notably, there is a known correlation between MAGE‑A9 expression and poor prognosis in HNSCC patients. However, current knowledge regarding the function of MAGE‑A9 expression, particularly in the context of irradiation, is limited. MAGE‑A9 expression in 37 oral squamous cell carcinoma patents was immunohistochemically determined and analyzed for overall survival by the Kaplan‑Meier log‑rank test. Next, the expression of MAGE‑A9 was determined by reverse transcription‑quantitative polymerase chain reaction in HNSCC cell lines prior to and following irradiation with 2 Gray. The radiosensitivity of each cell line was determined using a clonogenic survival assay. There was a significantly (P=0.0468) longer overall survival in patients with a low level of MAGE‑A9 expression. The median overall survival in patients with high MAGE‑A9 expression was 47% compared to 73% in the group with low MAGE‑A9 expression. The cell lines revealed a distinct expression pattern of MAGE‑A9. Following irradiation of the cell lines, a significant enhancement of MAGE‑A9 mRNA expression levels was observed. The most prominent alteration in MAGE‑A9 expression was observed in the most radioresistant cell line. A high MAGE‑A9 expression level correlates significantly with lower overall survival in HNSCC patients. Additionally, irradiation increased the MAGE‑A9 mRNA levels in all five HNSCC cell lines, and the most resistant cell line demonstrated the greatest increase in MAGE‑A9 expression following irradiation.
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March-2018
Volume 8 Issue 3

Print ISSN: 2049-9450
Online ISSN:2049-9469

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Spandidos Publications style
Meyer TJ, Hartmann S, Wohlleben G, Brisam M, Seher A, Kübler AC, Polat B and Müller‑Richter UD: MAGE-A9 in head and neck cancer: Prognostic value and preclinical findings in the context of irradiation. Mol Clin Oncol 8: 513-519, 2018
APA
Meyer, T.J., Hartmann, S., Wohlleben, G., Brisam, M., Seher, A., Kübler, A.C. ... Müller‑Richter, U.D. (2018). MAGE-A9 in head and neck cancer: Prognostic value and preclinical findings in the context of irradiation. Molecular and Clinical Oncology, 8, 513-519. https://doi.org/10.3892/mco.2018.1558
MLA
Meyer, T. J., Hartmann, S., Wohlleben, G., Brisam, M., Seher, A., Kübler, A. C., Polat, B., Müller‑Richter, U. D."MAGE-A9 in head and neck cancer: Prognostic value and preclinical findings in the context of irradiation". Molecular and Clinical Oncology 8.3 (2018): 513-519.
Chicago
Meyer, T. J., Hartmann, S., Wohlleben, G., Brisam, M., Seher, A., Kübler, A. C., Polat, B., Müller‑Richter, U. D."MAGE-A9 in head and neck cancer: Prognostic value and preclinical findings in the context of irradiation". Molecular and Clinical Oncology 8, no. 3 (2018): 513-519. https://doi.org/10.3892/mco.2018.1558