EGCG and Polyphenon E attenuate inflammation-related mouse colon carcinogenesis induced by AOM plus DDS

  • Authors:
    • Yohei Shirakami
    • Masahito Shimizu
    • Hisashi Tsurumi
    • Yukihiko Hara
    • Takuji Tanaka
    • Hisakata Moriwaki
  • View Affiliations

  • Published online on: May 1, 2008     https://doi.org/10.3892/mmr.1.3.355
  • Pages:355-361
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Abstract

Chronic inflammation raises the risk of the development of colorectal cancer (CRC). Green tea catechins (GTCs), which possess anti-inflammatory properties, are known to be anti-carcinogenic in a variety of organ sites, including the colorectum. This study investigated whether (−)-epigallocatechin gallate (EGCG), a candidate chemopreventive agent and major biologically-active component of green tea, and Polyphenon E (Poly E), a mixture of GTCs, suppress inflammation-related colon carcinogenesis induced by azoxymethane (AOM) and dextran sodium sulfate (DSS). Male ICR mice aged 5 weeks were given a single intraperitoneal injection of AOM (10 mg/kg body weight), followed by 2% (w/v) DSS in drinking water for 7 days to induce colitis-related colonic tumors. They also received drinking water containing EGCG (0.01 or 0.1%) or Poly E (0.01 or 0.1%) up to week 17. At week 17, treatment with EGCG or Poly E had significantly suppressed the multiplicity and volume of colonic neoplasms as compared to the AOM/DSS group, and had resulted in a lesser degree of malignancy. In addition, treatment with EGCG or Poly E decreased the protein and mRNA expression levels of Cyclooxygenase (COX)-2 and the mRNA expression of inflammatory cytokines (TNF-α, IFN-γ, IL-6, IL-12 and IL-18) in the colonic mucosa. Our findings provide evidence that tea catechins are beneficial to the suppression of cancer development in the inflamed colon.

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May 2008
Volume 1 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Shirakami, Y., Shimizu, M., Tsurumi, H., Hara, Y., Tanaka, T., & Moriwaki, H. (2008). EGCG and Polyphenon E attenuate inflammation-related mouse colon carcinogenesis induced by AOM plus DDS. Molecular Medicine Reports, 1, 355-361. https://doi.org/10.3892/mmr.1.3.355
MLA
Shirakami, Y., Shimizu, M., Tsurumi, H., Hara, Y., Tanaka, T., Moriwaki, H."EGCG and Polyphenon E attenuate inflammation-related mouse colon carcinogenesis induced by AOM plus DDS". Molecular Medicine Reports 1.3 (2008): 355-361.
Chicago
Shirakami, Y., Shimizu, M., Tsurumi, H., Hara, Y., Tanaka, T., Moriwaki, H."EGCG and Polyphenon E attenuate inflammation-related mouse colon carcinogenesis induced by AOM plus DDS". Molecular Medicine Reports 1, no. 3 (2008): 355-361. https://doi.org/10.3892/mmr.1.3.355