The application of autologous platelet‑rich plasma gel in cartilage regeneration

Xie,Aiguo; Nie,Lanjun; Shen,Gan; Cui,Ziwei; Xu,Peng; Ge,Huaqiang; Tan,Qian

doi:10.3892/mmr.2014.2358

The application of autologous platelet‑rich plasma gel in cartilage regeneration

Authors:
- Aiguo Xie
- Lanjun Nie
- Gan Shen
- Ziwei Cui
- Peng Xu
- Huaqiang Ge
- Qian Tan
View Affiliations

Affiliations: Department of Plastic Surgery, Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China, Department of Plastic Surgery, Drum Tower Clinical Medical College, Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China
Published online on: June 30, 2014 https://doi.org/10.3892/mmr.2014.2358
Pages: 1642-1648

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Cartilage defect caused by disease or trauma remains a challenge for surgeons, owning to the limited healing capacity of cartilage tissues. Cartilage tissue engineering provides a novel approach to address this issue, and appears promising for patients with cartilage defects. The cell scaffold, as one of the three key elements of tissue engineering, plays an important role in cartilage tissue engineering. Platelet‑rich plasma (PRP), which is a fraction of the plasma containing multiple growth factors, has become a major research focus in the context of its use as a bioactive scaffold for tissue engineering. Therefore, we investigated the value of using PRP scaffolds combined with chondrocytes in cartilage tissue engineering. In this study, we examined the levels of growth factors in PRP, and the effects of PRP on cell proliferation and matrix synthesis in rabbit chondrocytes cultured in PRP. Short-term in vitro culture followed by long‑term in vivo implantation was performed to evaluate the chondrogenesis of neocartilage in vivo. The results show that PRP may provide a suitable environment for the proliferation and maturation of chondrocytes, and can be used as a promising bioactive scaffold for cartilage regeneration.

View Figures

View References

1	Cao Y, Vacanti JP, Paige KT, Upton J and Vacanti CA: Transplantation of chondrocytes utilizing a polymer-cell construct to produce tissue-engineered cartilage in the shape of a human ear. Plast Reconstr Surg. 100:294–303. 1997.PubMed/NCBI
2	Luo X, Liu Y, Zhang Z, et al: Long-term functional reconstruction of segmental tracheal defect by pedicled tissue-engineered trachea in rabbits. Biomaterials. 34:3336–3344. 2013. View Article : Google Scholar : PubMed/NCBI
3	Wu J, Xue K, Li H, Sun J and Liu K: Improvement of PHBV scaffolds with bioglass for cartilage tissue engineering. PloS One. 8:e715632013. View Article : Google Scholar : PubMed/NCBI
4	Xue K, Zhu Y, Zhang Y, Chiang C, Zhou G and Liu K: Xenogeneic chondrocytes promote stable subcutaneous chondrogenesis of bone marrow-derived stromal cells. Int J Mol Med. 29:146–152. 2012.PubMed/NCBI
5	El Backly RM, Zaky SH, Canciani B, et al: Platelet rich plasma enhances osteoconductive properties of a hydroxyapatite-β-tricalcium phosphate scaffold (Skelite™) for late healing of critical size rabbit calvarial defects. J Craniomaxillofac Surg. Aug 7–2013.(Epub ahead of print).
6	Jiang ZQ, Liu HY, Zhang LP, Wu ZQ and Shang DZ: Repair of calvarial defects in rabbits with platelet-rich plasma as the scaffold for carrying bone marrow stromal cells. Oral Surg Oral Med Oral Pathol Oral Radiol. 113:327–333. 2012. View Article : Google Scholar
7	Kanthan SR, Kavitha G, Addi S, Choon DS and Kamarul T: Platelet-rich plasma (PRP) enhances bone healing in non-united critical-sized defects: a preliminary study involving rabbit models. Injury. 42:782–789. 2011. View Article : Google Scholar : PubMed/NCBI
8	Kilroy GE, Foster SJ, Wu X, et al: Cytokine profile of human adipose-derived stem cells: expression of angiogenic, hematopoietic, and pro-inflammatory factors. J Cell Physiol. 212:702–709. 2007. View Article : Google Scholar : PubMed/NCBI
9	Xue K, Qi L, Zhou G and Liu K: A two-step method of constructing mature cartilage using bone marrow-derived mesenchymal stem cells. Cells Tissues Organs. 197:484–495. 2013. View Article : Google Scholar : PubMed/NCBI
10	Björnsson S: Simultaneous preparation and quantitation of proteoglycans by precipitation with alcian blue. Anal Biochem. 210:282–291. 1993.PubMed/NCBI
11	Zhou G1, Liu W, Cui L, Wang X, Liu T and Cao Y: Repair of porcine articular osteochondral defects in non-weightbearing areas with autologous bone marrow stromal cells. Tissue Eng. 12:3209–3221. 2006. View Article : Google Scholar : PubMed/NCBI
12	Reddy GK and Enwemeka CS: A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem. 29:225–229. 1996. View Article : Google Scholar : PubMed/NCBI
13	Kuhne M, John T, El-Sayed K, et al: Characterization of auricular chondrocytes and auricular/articular chondrocyte co-cultures in terms of an application in articular cartilage repair. Int J Mol Med. 25:701–708. 2010.
14	Moon MH, Jeong JK, Lee YJ, Seol JW and Park SY: Sphingosine-1-phosphate inhibits interleukin-1β-induced inflammation in human articular chondrocytes. Int J Mol Med. 30:1451–1458. 2012.
15	Schubert T, Anders S, Neumann E, et al: Long-term effects of chondrospheres on cartilage lesions in an autologous chondrocyte implantation model as investigated in the SCID mouse model. Int J Mol Med. 23:455–460. 2009.
16	Sharma B, Fermanian S, Gibson M, et al: Human cartilage repair with a photoreactive adhesive-hydrogel composite. Sci Transl Med. 5:167ra1662013. View Article : Google Scholar : PubMed/NCBI
17	Vinatier C, Bouffi C, Merceron C, et al: Cartilage tissue engineering: towards a biomaterial-assisted mesenchymal stem cell therapy. Curr Stem Cell Res Ther. 4:318–329. 2009. View Article : Google Scholar : PubMed/NCBI
18	Akeda K, An HS, Okuma M, et al: Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis. Osteoarthritis Cartilage. 14:1272–1280. 2006. View Article : Google Scholar : PubMed/NCBI
19	Staudenmaier R, Froelich K, Birner M, et al: Optimization of platelet isolation and extraction of autogenous TGF-beta in cartilage tissue engineering. Artif Cells Blood Substit Immobil Biotechnol. 37:265–272. 2009. View Article : Google Scholar : PubMed/NCBI
20	Wu W, Zhang J, Dong Q, Liu Y, Mao T and Chen F: Platelet-rich plasma - a promising cell carrier for micro-invasive articular cartilage repair. Med Hypotheses. 72:455–457. 2009. View Article : Google Scholar : PubMed/NCBI
21	Arora R, Milz S, Sprecher C, Sitte I, Blauth M and Lutz M: Behaviour of ChronOS Inject in metaphyseal bone defects of distal radius fractures: tissue reaction after 6–15 months. Injury. 43:1683–1688. 2012.PubMed/NCBI
22	Pascual G, Rodriguez M, Sotomayor S, Perez-Kohler B and Bellon JM: Inflammatory reaction and neotissue maturation in the early host tissue incorporation of polypropylene prostheses. Hernia. 16:697–707. 2012. View Article : Google Scholar : PubMed/NCBI
23	Torrero JI, Aroles F and Ferrer D: Treatment of knee chondropathy with platelet rich plasma. Preliminary results at 6 months of follow-up with only one injection. J Biol Regul Homeost Agents. 26(2 Suppl 1): S71–S78. 2012.
24	Redaelli A, Romano D and Marciano A: Face and neck revitalization with platelet-rich plasma (PRP): clinical outcome in a series of 23 consecutively treated patients. J Drugs Dermatol. 9:466–472. 2010.PubMed/NCBI
25	Borrione P, Gianfrancesco AD, Pereira MT and Pigozzi F: Platelet-rich plasma in muscle healing. Am J Phys Med Rehabil. 89:854–861. 2010. View Article : Google Scholar : PubMed/NCBI
26	Yu W, Wang J and Yin J: Platelet-rich plasma: a promising product for treatment of peripheral nerve regeneration after nerve injury. Int J Neurosci. 121:176–180. 2011. View Article : Google Scholar : PubMed/NCBI
27	Centrella M, McCarthy TL and Canalis E: Effects of transforming growth factors on bone cells. Connect Tissue Res. 20:267–275. 1989. View Article : Google Scholar : PubMed/NCBI
28	Centrella M, McCarthy TL and Canalis E: Platelet-derived growth factor enhances deoxyribonucleic acid and collagen synthesis in osteoblast-enriched cultures from fetal rat parietal bone. Endocrinology. 125:13–19. 1989. View Article : Google Scholar
29	McCarthy TL, Centrella M and Canalis E: Regulatory effects of insulin-like growth factors I and II on bone collagen synthesis in rat calvarial cultures. Endocrinology. 124:301–309. 1989. View Article : Google Scholar : PubMed/NCBI
30	Canalis E, McCarthy TL and Centrella M: Effects of platelet-derived growth factor on bone formation in vitro. J Cell Physiol. 140:530–537. 1989. View Article : Google Scholar : PubMed/NCBI
31	Bastiaansen-Jenniskens YM, Koevoet W, de Bart AC, et al: Contribution of collagen network features to functional properties of engineered cartilage. Osteoarthritis Cartilage. 16:359–366. 2008. View Article : Google Scholar : PubMed/NCBI
32	Mikic B, Isenstein AL and Chhabra A: Mechanical modulation of cartilage structure and function during embryogenesis in the chick. Ann Biomed Eng. 32:18–25. 2004. View Article : Google Scholar : PubMed/NCBI