Berberine induces apoptosis and DNA damage in MG‑63 human osteosarcoma cells

Zhu,Yu; Ma,Nan; Li,Hui‑Xiang; Tian,Lin; Ba,Yu‑Feng; Hao,Bin

doi:10.3892/mmr.2014.2405

Berberine induces apoptosis and DNA damage in MG‑63 human osteosarcoma cells

Authors:
- Yu Zhu
- Nan Ma
- Hui‑Xiang Li
- Lin Tian
- Yu‑Feng Ba
- Bin Hao
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Affiliations: Department of Orthopedic Surgery, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China, Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Medicine, Zhengzhou Ninth People's Hospital, Zhengzhou, Henan 450014, P.R. China, Department of Thoracic Surgery, The Affiliated Tumor Hospital of Zhengzhou University (Henan Tumor Hospital), Zhengzhou, Henan 450008, P.R. China, Department of Urology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China
Published online on: July 21, 2014 https://doi.org/10.3892/mmr.2014.2405
Pages: 1734-1738
Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Berberine, an isoquinoline alkaloid extracted from the dry root of Coptidis Rhizoma, has been found to exhibit marked anticancer effects on a panel of established cancer cells. Among the human osteosarcoma lines treated, MG‑63 cells were found to be the most sensitive. The present study investigated the potential genotoxic effect of berberine on MG‑63 human osteosarcoma cells. The effect of berberine on cell viability was determined using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay and cell apoptosis was analyzed by flow cytometry and a DNA ladder assay. γH2AX focus formation was used to detect DNA damage in MG‑63 cells. Berberine induced a significant increase in apoptosis in MG‑63 cells in a concentration‑ and time‑dependent manner, as determined by DNA fragmentation analysis and flow cytometry. Furthermore, berberine induced signiﬁcant concentration‑ and time‑dependent increases in DNA damage compared with that in the negative control. In conclusion, these observations indicated that berberine induced apoptosis and DNA damage in MG‑63 cells.

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