Open Access

Neuroprotective effects of ginsenosides on neural progenitor cells against oxidative injury

  • Authors:
    • Jun Ye
    • Jian‑Ping Yao
    • Xu Wang
    • Minying Zheng
    • Peng Li
    • Chengwei He
    • Jian‑Bo Wan
    • Xiaoli Yao
    • Huanxing Su
  • View Affiliations

  • Published online on: February 19, 2016     https://doi.org/10.3892/mmr.2016.4914
  • Pages: 3083-3091
  • Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ginsenosides exhibit various neuroprotective effects against oxidative stress. However, which ginsenoside provides optimal effects for the treatment of neurological disorders as a potent antioxidant remains to be elucidated. Therefore, the present study investigated and compared the neuroprotective effects of the Rb1, Rd, Rg1 and Re ginsenosides on neural progenitor cells (NPCs) following tert-Butylhydroperoxide (t-BHP)-induced oxidative injury. Primary rat embryonic cortical NPCs were prepared from E14.5 embryos of Sprague-Dawley rats. The oxidative injury model was established with t‑BHP. A lactate dehydrogenase assay and terminal deoxynucleotidyl transferase dUTP nick‑end labeling staining were used to measure the viability of the NPCs pre‑treated with ginsenosides under oxidative stress. Reverse transcription‑quantitative polymerase chain reaction analysis was used to determine the activation of intracellular signaling pathways triggered by the pretreatment of ginsenosides. Among the four ginsenosides, only Rb1 attenuated t‑BHP toxicity in the NPCs, and the nuclear factor (erythroizd‑derived 2)‑like 2/heme oxygenase‑1 pathway was found to be key in the intracellular defense against oxidative stress. The present study demonstrated the anti-oxidative effects of ginsenoside Rb1 on NPCs, and suggested that Rb1 may offer potential as a potent antioxidant for the treatment of neurological disorders.
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April-2016
Volume 13 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ye J, Yao JP, Wang X, Zheng M, Li P, He C, Wan JB, Yao X and Su H: Neuroprotective effects of ginsenosides on neural progenitor cells against oxidative injury. Mol Med Rep 13: 3083-3091, 2016
APA
Ye, J., Yao, J., Wang, X., Zheng, M., Li, P., He, C. ... Su, H. (2016). Neuroprotective effects of ginsenosides on neural progenitor cells against oxidative injury. Molecular Medicine Reports, 13, 3083-3091. https://doi.org/10.3892/mmr.2016.4914
MLA
Ye, J., Yao, J., Wang, X., Zheng, M., Li, P., He, C., Wan, J., Yao, X., Su, H."Neuroprotective effects of ginsenosides on neural progenitor cells against oxidative injury". Molecular Medicine Reports 13.4 (2016): 3083-3091.
Chicago
Ye, J., Yao, J., Wang, X., Zheng, M., Li, P., He, C., Wan, J., Yao, X., Su, H."Neuroprotective effects of ginsenosides on neural progenitor cells against oxidative injury". Molecular Medicine Reports 13, no. 4 (2016): 3083-3091. https://doi.org/10.3892/mmr.2016.4914