Open Access

Ag85A/ESAT-6 chimeric DNA vaccine induces an adverse response in tuberculosis-infected mice

  • Authors:
    • Yan Liang
    • Xuejuang Bai
    • Junxian Zhang
    • Jingying Song
    • Yourong Yang
    • Qi Yu
    • Ning Li
    • Xueqiong Wu
  • View Affiliations

  • Published online on: June 3, 2016     https://doi.org/10.3892/mmr.2016.5364
  • Pages: 1146-1152
  • Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The Mycobacterium tuberculosis (M. tb) antigens encoded by the 6 kDa early secretory antigenic target (esat-6) and antigen 85A (ag85a) genes are known to exert protective effects against tuberculosis in animal models. In addition, these antigens represent vaccine components that were tested in early human clinical trials. In the present study, a chimeric DNA vaccine was constructed that contained two copies of the esat‑6 gene inserted into the ag85a gene from M. tb. BALB/c mice were treated with this chimeric vaccine following infection with either M. tb H37Rv or a clinical multi-drug-resistant tuberculosis isolate. Treatment of both groups of mice with the chimeric vaccine resulted in accelerated mortality. These findings are in contrast with previous results, which indicated that DNA vaccines expressing the individual antigens were either beneficial or at least not harmful. The results of the present study suggested that the ESAT-6 antigen is not suitable for inclusion in therapeutic vaccines.
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August-2016
Volume 14 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liang Y, Bai X, Zhang J, Song J, Yang Y, Yu Q, Li N and Wu X: Ag85A/ESAT-6 chimeric DNA vaccine induces an adverse response in tuberculosis-infected mice. Mol Med Rep 14: 1146-1152, 2016
APA
Liang, Y., Bai, X., Zhang, J., Song, J., Yang, Y., Yu, Q. ... Wu, X. (2016). Ag85A/ESAT-6 chimeric DNA vaccine induces an adverse response in tuberculosis-infected mice. Molecular Medicine Reports, 14, 1146-1152. https://doi.org/10.3892/mmr.2016.5364
MLA
Liang, Y., Bai, X., Zhang, J., Song, J., Yang, Y., Yu, Q., Li, N., Wu, X."Ag85A/ESAT-6 chimeric DNA vaccine induces an adverse response in tuberculosis-infected mice". Molecular Medicine Reports 14.2 (2016): 1146-1152.
Chicago
Liang, Y., Bai, X., Zhang, J., Song, J., Yang, Y., Yu, Q., Li, N., Wu, X."Ag85A/ESAT-6 chimeric DNA vaccine induces an adverse response in tuberculosis-infected mice". Molecular Medicine Reports 14, no. 2 (2016): 1146-1152. https://doi.org/10.3892/mmr.2016.5364