Secretory leukocyte protease inhibitor promotes differentiation and mineralization of MC3T3-E1 preosteoblasts on a titanium surface

Choi,Baik‑Dong; Lee,Seung‑Yeon; Jeong,Soon‑Jeong; Lim,Do‑Seon; Cha,Hee‑Jae; Chung,Won‑Gyun; Jeong,Moon‑Jin

doi:10.3892/mmr.2016.5381

Secretory leukocyte protease inhibitor promotes differentiation and mineralization of MC3T3-E1 preosteoblasts on a titanium surface

Authors:
- Baik‑Dong Choi
- Seung‑Yeon Lee
- Soon‑Jeong Jeong
- Do‑Seon Lim
- Hee‑Jae Cha
- Won‑Gyun Chung
- Moon‑Jin Jeong
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Affiliations: Department of Oral Histology and Developmental Biology, School of Dentistry, Chosun University, Gwangju 501‑759, Republic of Korea, Department of Dental Hygiene, Youngsan University, Yangsan, Gyeongsangnam‑do 626‑790, Republic of Korea, Department of Dental Hygiene, Eulji University, Seongnam, Gyeonggi 461‑713, Republic of Korea, Department of Parasitology and Genetics, College of Medicine, Kosin University, Busan 602‑072, Republic of Korea, Department of Dental Hygiene, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 220‑701, Republic of Korea
Published online on: June 9, 2016 https://doi.org/10.3892/mmr.2016.5381
Pages: 1241-1246

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Abstract

Mineralized bone matrix constituted with collagenous and non-collagenous proteins was synthesized by osteoblasts differentiated from mesenchymal stem cells. Secretory leukocyte protease inhibitor (SLPI), a serine protease inhibitor, promotes cell migration and proliferation, and suppresses the inflammatory response. Recent studies reported that SLPI regulates the formation of dentin and mineralization by odontoblasts and increases the adhesion and viability of preosteoblasts on a titanium (Ti) surface. Ti and its alloys are widely used implant materials in artificial joints and dental implants owing to their biocompatibility with bone. Therefore, this study aimed to examine whether SLPI can be an effective molecule in promoting differentiation and mineralization of osteoblasts on a Ti surface. In order to investigate the effects of SLPI on osteoblasts, an MTT assay, PCR, western blotting and Alizarin Red S staining were performed. The results demonstrated that SLPI increased the viability of osteoblasts during differentiation on Ti discs compared with that of the control. The expression levels of SLPI mRNA and protein were higher than that of the control after treatment of osteoblasts with SLPI on Ti discs during differentiation. SLPI increased the formation of mineralized nodules and mRNA expression of alkaline phosphatase, dentin sialophosphoprotein, dentin matrix protein 1, bone sialoprotein, and collagen I in osteoblasts on Ti discs compared with that of the control. In conclusion, SLPI increases the viability and promotes the differentiation and mineralization of osteoblasts on Ti surfaces, suggesting that SLPI is an effective molecule for achieving successful osseointegration between osteoblasts and a Ti surface.

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