Protein kinase C α is involved in the regulation of AXL receptor tyrosine kinase expression in triple-negative breast cancer cells

Yue,Chia‑Herng; Liu,Liang‑Chih; Kao,Erl‑Shyh; Lin,Ho; Hsu,Li‑Sung; Hsu,Chih‑Wei; Lin,Yu‑Yu; Lin,Yi‑Syuan; Liu,Jer‑Yuh; Lee,Chia‑Jen

doi:10.3892/mmr.2016.5424

Protein kinase C α is involved in the regulation of AXL receptor tyrosine kinase expression in triple-negative breast cancer cells

Authors:
- Chia‑Herng Yue
- Liang‑Chih Liu
- Erl‑Shyh Kao
- Ho Lin
- Li‑Sung Hsu
- Chih‑Wei Hsu
- Yu‑Yu Lin
- Yi‑Syuan Lin
- Jer‑Yuh Liu
- Chia‑Jen Lee
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Affiliations: Department of Surgery, Tungs' Taichung Metroharbor Hospital, Taichung 435, Taiwan, R.O.C., Department of Surgery, China Medical University Hospital, Changhua 404, Taiwan, R.O.C., Department of Beauty Science and Graduate Institute of Beauty Science Technology, Chienkuo Technology University, Taichung 500, Taiwan, R.O.C., Department of Life Science, National Chung Hsing University, Taichung 40402, Taiwan, R.O.C., Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung 404, Taiwan, R.O.C., Graduate Institute of Cancer Biology, College of Medicine, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Biotechnology, Asia University, Taichung 413, Taiwan, R.O.C., Center for Molecular Medicine, China Medical University Hospital, Taichung 404, Taiwan, R.O.C.
Published online on: June 23, 2016 https://doi.org/10.3892/mmr.2016.5424
Pages: 1636-1642

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Abstract

AXL receptor tyrosine kinase is overexpressed in triple-negative breast cancer (TNBC), and has a function in cancer progression and metastases. However, the mechanism underlying AXL gene regulation in TNBC remains unknown. In this study, the involvement of protein kinase C α (PKCα) in the expression of AXL was investigated in human TNBC cells. The microarray data from other studies showed that PKCα is significantly correlated with AXL expression in TNBC cell lines. Tissue array analysis also confirmed their correlation in TNBC. The PKCα inhibitor Go6976 was used to treat MDA‑MB‑231 and Hs578T TNBC cells, which resulted in decreased expression of AXL and epithelia-mesenchymal transition-related gene vimentin, and decreased cell proliferation. An MZF‑1 acidic domain fragment (MZF-1 peptide), which was designed to downregulate PKCα expression, was transfected into the cells and resulted in inhibition of AXL expression. This effect was reversed by co‑treatment with the constitutive form of PKCα. Moreover, the downregulation of PKCα was also confirmed by treatment with TAT‑fused MZF‑1 peptide. Thus, the current study proposes that AXL may be correlated with PKCα‑dependent TNBC cells, and could be modulated by MZF‑1 peptides.

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