The expression pattern of Adam10 in the central nervous system of adult mice: Detection by in situ hybridization combined 
with immunohistochemistry staining

Guo,Zhi‑Bao; Su,Ying‑Ying; Wang,Yi‑Hui; Wang,Wei; Guo,Da‑Zhi

doi:10.3892/mmr.2016.5501

The expression pattern of Adam10 in the central nervous system of adult mice: Detection by in situ hybridization combined with immunohistochemistry staining

Authors:
- Zhi‑Bao Guo
- Ying‑Ying Su
- Yi‑Hui Wang
- Wei Wang
- Da‑Zhi Guo
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Affiliations: Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China, Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Hyperbaric Oxygen, Navy General Hospital, Beijing 100048, P.R. China
Published online on: July 11, 2016 https://doi.org/10.3892/mmr.2016.5501
Pages: 2038-2044
Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

ADAM10 (a disintegrin and metalloprotease 10) is a member of the ADAMs family, which is key in the development of the nervous system, by regulating proliferation, migration, differentiation and survival of various cells, including axonal growth and myelination. Previous studies have investigated the embryonic or postnatal expression of ADAM10, however, detailed information regarding its cellular distribution in the adult stage, to the best of our knowledge, is not available. The present study investigated the expression pattern of the ADAM10 gene in the adult mouse central nervous system (CNS) using an ADAM10 complementary RNA probe for in situ hybridization (ISH). Immunohistochemical staining was used to identify the type of the ISH staining‑positive cells with neuron‑ or astrocyte‑specific antibodies. The results of the current study demonstrated that the ADAM10 gene was predominantly expressed in the neurons of the cerebral cortex, hippocampus, thalamus and cerebellar granular cells in adult mouse CNS.

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