Berberine enhances the anti‑tumor activity of tamoxifen in drug‑sensitive MCF‑7 and drug‑resistant MCF‑7/TAM cells

  • Authors:
    • Chunjie Wen
    • Lanxiang Wu
    • Lijuan Fu
    • Xue Zhang
    • Honghao Zhou
  • View Affiliations

  • Published online on: July 8, 2016     https://doi.org/10.3892/mmr.2016.5490
  • Pages: 2250-2256
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Abstract

Berberine, an isoquinoline alkaloid, has been previously demonstrated to possess anti‑breast cancer properties. Tamoxifen is widely used in the prevention and treatment of estrogen receptor-positive breast cancer. Thus, the aim of the present study was to assess whether berberine enhanced the anticancer effect of tamoxifen, and the underlying mechanism involved in this combined effect in tamoxifen-sensitive (MCF-7) and tamoxifen-resistant (MCF-7/TAM) cells using MTS, flow cytometry and western blot assays. The results indicated that berberine demonstrated dose‑ and time‑dependent anti‑proliferative activity in MCF‑7 and MCF‑7/TAM cells. Furthermore, the combination of berberine and tamoxifen induced cell growth inhibition more effectively than tamoxifen alone. The present study also demonstrated that combinational treatment is more effective in inducing G1 phase arrest and activating apoptosis compared tamoxifen alone, which may be due to upregulation of P21 expression and downregulation of the B‑cell CLL/lymphoma 2(Bcl‑2)/Bcl‑2 associated X protein ratio. The results of the present study suggested that berberine may potentially be useful as an adjuvant agent in cancer chemotherapy to enhance the effect of tamoxifen, which will be useful for anti‑tumor therapy and further research.
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September-2016
Volume 14 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wen C, Wu L, Fu L, Zhang X and Zhou H: Berberine enhances the anti‑tumor activity of tamoxifen in drug‑sensitive MCF‑7 and drug‑resistant MCF‑7/TAM cells. Mol Med Rep 14: 2250-2256, 2016.
APA
Wen, C., Wu, L., Fu, L., Zhang, X., & Zhou, H. (2016). Berberine enhances the anti‑tumor activity of tamoxifen in drug‑sensitive MCF‑7 and drug‑resistant MCF‑7/TAM cells. Molecular Medicine Reports, 14, 2250-2256. https://doi.org/10.3892/mmr.2016.5490
MLA
Wen, C., Wu, L., Fu, L., Zhang, X., Zhou, H."Berberine enhances the anti‑tumor activity of tamoxifen in drug‑sensitive MCF‑7 and drug‑resistant MCF‑7/TAM cells". Molecular Medicine Reports 14.3 (2016): 2250-2256.
Chicago
Wen, C., Wu, L., Fu, L., Zhang, X., Zhou, H."Berberine enhances the anti‑tumor activity of tamoxifen in drug‑sensitive MCF‑7 and drug‑resistant MCF‑7/TAM cells". Molecular Medicine Reports 14, no. 3 (2016): 2250-2256. https://doi.org/10.3892/mmr.2016.5490