Inflammation has a role in urethane‑induced lung cancer in C57BL/6J mice

Xu,Cai; Zhou,Lingyu; Lu,Lei; Chen,Ting; Wei,Siyu; Lin,Xiaojing; Lian,Xuemei

doi:10.3892/mmr.2016.5661

Inflammation has a role in urethane‑induced lung cancer in C57BL/6J mice

Authors:
- Cai Xu
- Lingyu Zhou
- Lei Lu
- Ting Chen
- Siyu Wei
- Xiaojing Lin
- Xuemei Lian
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Affiliations: School of Public Health and Management, Research Center for Medicine and Social Development, Innovation Center for Social Risk Governance in Health, Chongqing Medical University, Chongqing 400016, P.R. China, Department of Prevention and Control of Chronic Diseases, Center for Disease Control and Prevention in Chongqing, Chongqing 400016, P.R. China
Published online on: August 19, 2016 https://doi.org/10.3892/mmr.2016.5661
Pages: 3323-3328

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Abstract

Lung cancer is a common and highly frequent cause of cancer‑associated mortality worldwide. Several studies have indicated that chronic inflammation is associated with an increased risk of several types of human cancer. The lung is vulnerable to various chemical and biological insults, and persistent exposure to these factors may result in the release of several inflammatory cytokines from inflammatory cells, thus leading to chronic inflammation and a risk of lung cancer. Due to the extensive application of C57BL/6J mice in lipid metabolism‑related research, it appears important to establish a lung cancer model based on C57BL/6J mice. Therefore, the present study designed an experimental model, in which C57BL/6J mice received several injections of urethane. The study aimed to explore whether inflammation has a role in this model of lung cancer. The results demonstrated that 10 weekly intraperitoneal injections of urethane induced a 100% lung tumor incidence, and urethane‑treated mice possessed higher numbers of immune cells. In addition, the expression levels of cytokines and chemokines in bronchoalveolar lavage fluid were significantly different between the two groups. Activation of the transcription factor nuclear factor‑κB was increased in the lung tissues of urethane‑treated mice, and its expression was upregulated in a time‑dependent manner. These results suggested that the accumulation of lung inflammation may be associated with the occurrence of lung cancer in C57BL/6J mice.

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