Differential activation of c‑Fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats

Tae,Hyun‑Jin; Park,Seung Min; Cho,Jeong Hwi; Kim,In Hye; Ahn,Ji Hyeon; Park,Joon Ha; Won,Moo‑Ho; Chen,Bai Hui; Shin,Bich‑Na; Shin,Myoung Cheol; Lee,Choong Hyun; Hong,Seongkweon; Lee,Jae‑Chul; Cho,Jun Hwi

doi:10.3892/mmr.2016.5731

Differential activation of c‑Fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats

Authors:
- Hyun‑Jin Tae
- Seung Min Park
- Jeong Hwi Cho
- In Hye Kim
- Ji Hyeon Ahn
- Joon Ha Park
- Moo‑Ho Won
- Bai Hui Chen
- Bich‑Na Shin
- Myoung Cheol Shin
- Choong Hyun Lee
- Seongkweon Hong
- Jae‑Chul Lee
- Jun Hwi Cho
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Affiliations: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Emergency Medicine, Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, Gyeonggi 14068, Republic of Korea, Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Physiology, College of Medicine, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, Chungcheongnam 16890, Republic of Korea, Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea
Published online on: September 7, 2016 https://doi.org/10.3892/mmr.2016.5731
Pages: 3503-3508
Copyright: © Tae et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Proto-oncogene c‑Fos (c‑Fos) is frequently used to detect a pathogenesis in central nervous system disorders. The present study examined changes in the immunoreactivity of c‑Fos in the paraventricular nucleus of the hypothalamus (PVNH) and paraventricular nucleus of the thalamus (PVNT) following myocardial infarction (MI) in rats. Infarction in the left ventricle was examined by Masson's trichrome staining. Neuronal degeneration was monitored for 56 days after MI using crystal violet and Fluoro‑Jade B histofluorescence staining. Changes in the immunoreactivity of c‑Fos were determined using immunohistochemistry for c‑Fos. The average infarct size of the left ventricle circumference was ~44% subsequent to MI. Neuronal degeneration was not detected in PVNH and PVNT following MI. c‑Fos immunoreactive (+) cells were infrequently observed in the nuclei of the sham‑group. However, the number of c‑Fos+ cells was increased in the nuclei following MI and peaked in the PVNH and PVNT at 3 and 14 days, respectively. The number of c‑Fos+ cells were comparable with the sham group at 56 days after MI. Therefore, MI may induce c‑Fos immunoreactivity in PVNH and PVNT, this increase of c‑Fos expression levels may be associated with the stress that occurs in the brain following MI.

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