p53, Bcl-2 and cox-2 are involved in berberine hydrochloride-induced apoptosis of HeLa229 cells

  • Authors:
    • Hai‑Yan Wang
    • Hai‑Zhong Yu
    • Sheng‑Mou Huang
    • Yu‑Lan Zheng
  • View Affiliations

  • Published online on: August 31, 2016     https://doi.org/10.3892/mmr.2016.5696
  • Pages: 3855-3861
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Abstract

The present study aimed to investigate the effects of berberine hydrochloride on the proliferation and apoptosis of HeLa229 human cervical cancer cells. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to examine the cytotoxicity of berberine hydrochloride against HeLa229 cells. The effects of berberine hydrochloride on the apoptosis of HeLa229 cells was detected by immunofluorescence and flow cytometry, and the mRNA expression levels of p53, B‑cell lymphoma 2 (Bcl‑2) and cyclooxygenase‑2 (cox‑2) were analyzed by reverse transcription-quantitative polymerase chain reaction. Berberine hydrochloride inhibited the proliferation of HeLa229 cells in a dose‑dependent manner; minimum cell viability (3.61%) was detected following treatment with 215.164 µmol/l berberine hydrochloride and the half maximal inhibitory concentration value was 42.93 µmol/l following treatment for 72 h. In addition, berberine hydrochloride induced apoptosis in HeLa229 cells in a dose‑ and time‑dependent manner. Berberine hydrochloride upregulated the mRNA expression levels of p53, and downregulated mRNA expression levels of Bcl‑2 and cox‑2, in a dose‑dependent manner. In conclusion, berberine hydrochloride inhibited the proliferation and induced apoptosis of HeLa229 cells, potentially via the upregulation of p53 and the downregulation of Bcl‑2 and cox‑2 mRNA expression levels.
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October-2016
Volume 14 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang HY, Yu HZ, Huang SM and Zheng YL: p53, Bcl-2 and cox-2 are involved in berberine hydrochloride-induced apoptosis of HeLa229 cells. Mol Med Rep 14: 3855-3861, 2016
APA
Wang, H., Yu, H., Huang, S., & Zheng, Y. (2016). p53, Bcl-2 and cox-2 are involved in berberine hydrochloride-induced apoptosis of HeLa229 cells. Molecular Medicine Reports, 14, 3855-3861. https://doi.org/10.3892/mmr.2016.5696
MLA
Wang, H., Yu, H., Huang, S., Zheng, Y."p53, Bcl-2 and cox-2 are involved in berberine hydrochloride-induced apoptosis of HeLa229 cells". Molecular Medicine Reports 14.4 (2016): 3855-3861.
Chicago
Wang, H., Yu, H., Huang, S., Zheng, Y."p53, Bcl-2 and cox-2 are involved in berberine hydrochloride-induced apoptosis of HeLa229 cells". Molecular Medicine Reports 14, no. 4 (2016): 3855-3861. https://doi.org/10.3892/mmr.2016.5696