Integrated microRNA and protein expression analysis reveals novel microRNA regulation of targets in fetal down syndrome

Lin,Hua; Sui,Weiguo; Li,Wuxian; Tan,Qiupei; Chen,Jiejing; Lin,Xiuhua; Guo,Hui; Ou,Minglin; Xue,Wen; Zhang,Ruohan; Dai,Yong

doi:10.3892/mmr.2016.5775

Integrated microRNA and protein expression analysis reveals novel microRNA regulation of targets in fetal down syndrome

Authors:
- Hua Lin
- Weiguo Sui
- Wuxian Li
- Qiupei Tan
- Jiejing Chen
- Xiuhua Lin
- Hui Guo
- Minglin Ou
- Wen Xue
- Ruohan Zhang
- Yong Dai
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Affiliations: Nephrology Department of the 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi 541002, P.R. China, The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, P.R. China
Published online on: September 26, 2016 https://doi.org/10.3892/mmr.2016.5775
Pages: 4109-4118
Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Down syndrome (DS) is caused by trisomy of human chromosome 21 and is associated with a number of deleterious phenotypes. To investigate the role of microRNA (miRNA) in the regulation of DS, high‑throughput Illumina sequencing technology and isobaric tagging for relative and absolute protein quantification analysis were utilized for simultaneous expression profiling of miRNA and protein in fetuses with DS and normal fetuses. A total of 344 miRNAs were associated with DS. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were used to investigate the proteins found to be differentially expressed. Functionally important miRNAs were determined by identifying enriched or depleted targets in the transcript and the protein expression levels were consistent with miRNA regulation. The results indicated that GRB2, TMSB10, RUVBL2, the hsa‑miR‑329 and hsa‑miR‑27b, hsa‑miR‑27a targets, and MAPK1, PTPN11, ACTA2 and PTK2 or other differentially expressed proteins were connected with each other directly or indirectly. Integrative analysis of miRNAs and proteins provided an expansive view of the molecular signaling pathways in DS.

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