NF-κB inhibition attenuates LPS-induced TLR4 activation in monocyte cells

Wan,Jian; Shan,Yi; Fan,Yibo; Fan,Conghui; Chen,Song; Sun,Jie; Zhu,Lili; Qin,Long; Yu,Mengjin; Lin,Zhaofen

doi:10.3892/mmr.2016.5825

NF-κB inhibition attenuates LPS-induced TLR4 activation in monocyte cells

Authors:
- Jian Wan
- Yi Shan
- Yibo Fan
- Conghui Fan
- Song Chen
- Jie Sun
- Lili Zhu
- Long Qin
- Mengjin Yu
- Zhaofen Lin
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Affiliations: Department of Emergency and Critical Care Medicine, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China, Department of Emergency and Critical Care Medicine, The People's Hospital of Pudong New Area, Shanghai University of Health and Science, Shanghai 201200, P.R. China
Published online on: October 12, 2016 https://doi.org/10.3892/mmr.2016.5825
Pages: 4505-4510
Copyright: © Wan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Toll-like receptor (TLR) family are receptors for extracellular or intracellular signaling, such as lipopolysaccharide (LPS), or 12-O-tetradecanoylphorbol-13-acetate. TLR induces the differentiation of human myeloid monocytic‑leukemia cells (THP-1) to macrophages. However, the relationship between extracellular or intracellular signaling and the TLR protein level remain to be determined. Using RT-PCR and western blot analysis, the aim of the present study was to determine whether TLR4, a major TLR family member, could be moderately upregulated by high concentration of LPS and whether it promoted the maturation of THP1 cells. The results showed that, upregulated TLR4 at the protein level and mRNA level enriched the TLR4 modulation style. In addition, TLR4 expression was blocked by nuclear factor (NF)-κB inhibitor, and LPS stimulated NF-κB binding in the TLR4 gene promoter. Therefore, the increased expression of TLR4 in the responsiveness of LPS-treated THP1 cells occurred in response to the upregulation of their respective receptors, as well as a tight binding of NF-κB in the TLR4 gene promoter.

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