Identification and bioinformatic analysis of dysregulated microRNAs in human oligodendroglial cells infected with borna disease virus

Zhang,Hong; He,Peng; Huang,Rongzhong; Sun,Lin; Liu,Siwen; Zhou,Jingjing; Guo,Yujie; Yang,Deyu; Xie,Peng

doi:10.3892/mmr.2016.5842

Identification and bioinformatic analysis of dysregulated microRNAs in human oligodendroglial cells infected with borna disease virus

Authors:
- Hong Zhang
- Peng He
- Rongzhong Huang
- Lin Sun
- Siwen Liu
- Jingjing Zhou
- Yujie Guo
- Deyu Yang
- Peng Xie
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Affiliations: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China, Department of Rehabilitation, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing 400016, P.R. China
Published online on: October 13, 2016 https://doi.org/10.3892/mmr.2016.5842
Pages: 4715-4722

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Abstract

MicroRNAs (miRNAs) are recognized as important regulators of gene expression via translational depression or mRNA degradation. Previously, dysregulated miRNAs have been found in neurodegenerative and neuropsychiatric disorders. Borna disease virus (BDV) is a neurotropic, negative single‑stranded RNA virus, which may be a cause of human neuropsychiatric disease. BDV is regarded as an ideal model to analyze the molecular mechanisms of mental disorders caused by viral infection. In the present study, 10 miRNAs were dysregulated in human oligodendrocytes (OL cells) infected with the BDV strain, Hu‑H1 (OL/BDV). The predicted target genes of those different miRNAs were closely associated with DNA binding, receptor activity, cytoplasm and membrane, biopolymer metabolic process and signal transduction, which were ranked highest using Gene Ontology (GO) analysis, and were predominantly involved in ‘Immune system and adaptive Immune system pathways’ on pathway analysis. Reverse transcription‑quantitative polymerase chain reaction analysis confirmed that seven miRNAs (miR‑1290, miR‑1908, miR‑146a‑5p, miR‑424‑5p, miR‑3676‑3p, miR‑296‑3p and miR‑7‑5p) were significantly downregulated in the OL/BDV cells, whereas two miRNAs (miR‑1244 and miR‑4521) showed no significant differences between the two groups. The present study revealed for the first time, to the best of our knowledge, the miRNA profile of BDV Hu‑H1‑infected human OL cells. Based on GO and pathway analyses, further investigation of the signaling processes in BDV‑infected oligodendrocytes may offer particular promise in improving understanding of the neuropathogenesis of BDV.

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