Decreased expression of miR‑9 due to E50K OPTN mutation causes disruption of the expression of BDNF leading to RGC‑5 cell apoptosis

Jiang,Bo; Gao,Lin; Lei,Dawei; Liu,Jiannan; Shao,Zhengbo; Zhou,Xinrong; Li,Renke; Wu,Donglai; Xue,Fei; Zhu,Yuanmao; Yuan,Huiping

doi:10.3892/mmr.2016.5810

Decreased expression of miR‑9 due to E50K OPTN mutation causes disruption of the expression of BDNF leading to RGC‑5 cell apoptosis

Authors:
- Bo Jiang
- Lin Gao
- Dawei Lei
- Jiannan Liu
- Zhengbo Shao
- Xinrong Zhou
- Renke Li
- Donglai Wu
- Fei Xue
- Yuanmao Zhu
- Huiping Yuan
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Affiliations: Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Division of Cardiovascular Surgery and Toronto General Research Institute, University Health Network and Department of Surgery, Division of Cardiac Surgery, University of Toronto, Toronto, ON M5G 1L7, Canada, The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150001, P.R. China
Published online on: October 6, 2016 https://doi.org/10.3892/mmr.2016.5810
Pages: 4901-4905

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Abstract

The aims of the present study were to investigate the effect of E50K optineurin (OPTN) mutation on RGC‑5 cells and to define the role of microRNA‑9 (miR‑9) in this system. Transfected RGC‑5 cells were used to evaluate the effects of E50K OPTN on the expression of miR‑9 and subsequent disruption of RGC‑5 cell apoptosis was analyzed using western blotting. The results showed that the expression of E50K OPTN was associated with a marked reduction in the levels of miR‑9 in the E50K OPTN‑transfected RGC‑5 cells. The E50K OPTN‑dependent reductions in miR‑9 led to increased expression of the transcriptional repressor, RE1‑silencing transcription factor and decreased the expression of brain‑derived neurotrophic factor. Thus, E50K OPTN may disrupt the expression of miR‑9, suggesting a potential mechanism by which E50K OPTN mutation may lead to RGC‑5 cell apoptosis.

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