Roles of mitochondrial transcription factor A and microRNA‑590‑3p in the development of colon cancer

Wu,Kaiming; Zhao,Zhenxian; Xiao,Yinglian; Peng,Jianjun; Chen,Jianhui; He,Yulong

doi:10.3892/mmr.2016.5955

Roles of mitochondrial transcription factor A and microRNA‑590‑3p in the development of colon cancer

Authors:
- Kaiming Wu
- Zhenxian Zhao
- Yinglian Xiao
- Jianjun Peng
- Jianhui Chen
- Yulong He
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Affiliations: Department of Gastrointestinal Surgery, First Affiliated Hospital of Sun Yat‑Sen Univesity, Guangzhou, Guangdong 510080, P.R. China, Department of Pancreato‑Biliary Surgery, First Affiliated Hospital of Sun Yat‑Sen Univesity, Guangzhou, Guangdong 510080, P.R. China, Department of Gastroenterology and Hepatology, First Affiliated Hospital of Sun Yat‑Sen Univesity, Guangzhou, Guangdong 510080, P.R. China
Published online on: November 21, 2016 https://doi.org/10.3892/mmr.2016.5955
Pages: 5475-5480
Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mitochondrial transcription factor A (TFAM) participates in the process of mitochondrial DNA replication and transcription. microRNAs (miRNAs) serve an important role in the regulation of gene expression. However, the roles of TFAM and certain miRNAs and their associations in the development of numerous cancer types remain unclear. The current study demonstrated that the expression of TFAM was significantly upregulated in colon cancer compared with the normal tissue, while the expression of miRNA‑590‑3p (miR‑590‑3p) was predicted with a high score using miRWalk software, and the luciferase assay demonstrated that TFAM was the direct target of miRNA‑590‑3p. miR‑590‑3p exhibited high expression levels in both colon cancer tissue and the SW480 cell line. Furthermore, downregulated expression of miR‑590‑3p significantly inhibited the growth of SW480 cells, which was consistent with results indicating downregulated expression of TFAM in SW480 cells from a previous study. In summary, the results of the current study concluded that miR‑590‑3p, via direct targeting of TFAM, may serve an important role in the tumorigenesis of colon cancer, and may be a promising target for colon cancer therapeutics.

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1	http://www.who.int/mediacentre/factsheets/fs297/en/Accessed on 29 August 2012.
2	Bray F, Ren JS, Masuyer E and Ferlay J: Global estimates of cancer prevalence for 27 sites in the adult population in 2008. Int J Cancer. 132:1133–1145. 2013. View Article : Google Scholar : PubMed/NCBI
3	Bonawitz ND, Clayton DA and Shadel GS: Initiation and beyond: Multiple functions of the human mitochondrial transcription machinery. Mol Cell. 24:813–825. 2006. View Article : Google Scholar : PubMed/NCBI
4	Parisi MA and Clayton DA: Similarity of human mitochondrial transcription factor 1 to high mobility group proteins. Science. 252:965–969. 1991. View Article : Google Scholar : PubMed/NCBI
5	Litonin D, Sologub M, Shi Y, Savkina M, Anikin M, Falkenberg M, Gustafsson CM and Temiakov D: Human mitochondrial transcription revisited: Only TFAM and TFB2M are required for transcription of the mitochondrial genes in vitro. J Biol Chem. 285:18129–18133. 2010. View Article : Google Scholar : PubMed/NCBI
6	Tang D, Kang R, Zeh HJ III and Lotze MT: High-mobility group box 1 and cancer. Biochim Biophys Acta. 1799:131–140. 2010. View Article : Google Scholar : PubMed/NCBI
7	Baffy G: Uncoupling protein-2 and cancer. Mitochondrion. 10:243–252. 2010. View Article : Google Scholar : PubMed/NCBI
8	Yoshida Y, Hasegawa J, Nezu R, Kim YK, Hirota M, Kawano K, Izumi H and Kohno K: Clinical usefulness of mitochondrial transcription factor a expression as a predictive marker in colorectal cancer patients treated with FOLFOX. Cancer Sci. 102:578–582. 2011. View Article : Google Scholar : PubMed/NCBI
9	Toki N, Kagami S, Kurita T, Kawagoe T, Matsuura Y, Hachisuga T, Matsuyama A, Hashimoto H, Izumi H and Kohno K: Expression of mitochondrial transcription factor A in endometrial carcinomas: Clinicopathologic correlations and prognostic significance. Virchows Arch. 456:387–393. 2010. View Article : Google Scholar : PubMed/NCBI
10	Alam TI, Kanki T, Muta T, Ukaji K, Abe Y, Nakayama H, Takio K, Hamasaki N and Kang D: Human mitochondrial DNA is packaged with TFAM. Nucleic Acids Res. 31:1640–1645. 2003. View Article : Google Scholar : PubMed/NCBI
11	Kaufman BA, Durisic N, Mativetsky JM, Costantino S, Hancock MA, Grutter P and Shoubridge E: The mitochondrial transcription factor TFAM coordinates the assembly of multiple DNA molecules into nucleoid-like structures. Mol Biol Cell. 18:3225–3236. 2007. View Article : Google Scholar : PubMed/NCBI
12	Xu S, Zhong M, Zhang L, Wang Y, Zhou Z, Hao Y, Zhang W, Yang X, Wei A, Pei L and Yu Z: Overexpression of Tfam protects mitochondria against beta-amyloid-induced oxidative damage in SH-SY5Y cells. FEBS J. 276:3800–3809. 2009. View Article : Google Scholar : PubMed/NCBI
13	Aydin J, Andersson DC, Hänninen SL, Wredenberg A, Tavi P, Park CB, Larsson NG, Bruton JD and Westerblad H: Increased mitochondrial Ca2+ and decreased sarcoplasmic reticulum Ca2+ in mitochondrial myopathy. Hum Mol Genet. 18:278–288. 2009. View Article : Google Scholar : PubMed/NCBI
14	Kidani A, Izumi H, Yoshida Y, Kashiwagi E, Ohmori H, Tanaka T, Kuwano M and Kohno K: Thioredoxin2 enhances the damaged DNA binding activity of mtTFA through direct interaction. Int J Oncol. 35:1435–1440. 2009.PubMed/NCBI
15	Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI
16	Petersen CP, Bordeleau ME, Pelletier J and Sharp PA: Short RNAs repress translation after initiation in mammalian cells. Mol Cell. 21:533–542. 2006. View Article : Google Scholar : PubMed/NCBI
17	Adam L, Zhong M, Choi W, Qi W, Nicoloso M, Arora A, Calin G, Wang H, Siefker-Radtke A, McConkey D, et al: MiR-200 expression regulates epithelial-to-mesenchymal transition in bladder cancer cells and reverses resistance to epidermal growth factor receptor therapy. Clin Cancer Res. 15:5060–5072. 2009. View Article : Google Scholar : PubMed/NCBI
18	Saito Y, Suzuki H, Tsugawa H, Nakagawa I, Matsuzaki J, Kanai Y and Hibi T: Chromatin remodeling at Alu repeats by epigenetic treatment activates silenced microRNA-512-5p with down regulation of Mcl-1 in human gastric cancer cells. Oncogene. 28:2738–2744. 2009. View Article : Google Scholar : PubMed/NCBI
19	Mazar J, DeYoung K, Khaitan D, Meister E, Almodovar A, Goydos J, Ray A and Perera RJ: The regulation of miRNA-211 expression and its role in melanoma cell invasiveness. PLoS One. 5:e137792010. View Article : Google Scholar : PubMed/NCBI
20	Di Leva G, Piovan C, Gasparini P, Ngankeu A, Taccioli C, Briskin D, Cheung DG, Bolon B, Anderlucci L, Alder H, et al: Estrogen mediated-activation of miR-191/425 cluster modulates tumorigenicity of breast cancer cells depending on estrogen receptor status. PLoS Genet. 9:e10033112013. View Article : Google Scholar : PubMed/NCBI
21	Rivas MA, Venturutti L, Huang YW, Schillaci R, Huang TH and Elizalde PV: Downregulation of the tumor-suppressor miR-16 via progestin-mediated oncogenic signaling contributes to breast cancer development. Breast Cancer Res. 14:R772012. View Article : Google Scholar : PubMed/NCBI
22	Kalinowski FC, Giles KM, Candy PA, Ali A, Ganda C, Epis MR, Webster RJ and Leedman PJ: Regulation of epidermal growth factor receptor signaling and erlotinib sensitivity in head and neck cancer cells by miR-7. PLoS One. 7:e470672012. View Article : Google Scholar : PubMed/NCBI
23	Villa C, Fenoglio C, De Riz M, Clerici F, Marcone A, Benussi L, Ghidoni R, Gallone S, Cortini F, Serpente M, et al: Role of hnRNP-A1 and miR-590-3p in neuronal death: Genetics and expression analysis in patients with Alzheimer disease and frontotemporal lobar degeneration. Rejuvenation Res. 14:275–281. 2011. View Article : Google Scholar : PubMed/NCBI
24	Vinuesa CG, Rigby RJ and Yu D: Logic and extent of miRNA-mediated control of autoimmune gene expression. Int Rev Immunol. 28:112–138. 2009. View Article : Google Scholar : PubMed/NCBI
25	Benson AB III, Venook AP, Bekaii-Saab T, Chan E, Chen YJ, Cooper HS, Engstrom PF, Enzinger PC, Fenton MJ, Fuchs CS, et al: National Comprehensive Cancer Network: Colon cancer, version 3.2014. J Natl Compr Canc Netw. 12:1028–1059. 2014.PubMed/NCBI
26	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
27	Belyavsky A, Vinogradova T and Rajewsky K: PCR-based cDNA library construction: General cDNA libraries at the level of a few cells. Nucleic Acids Res. 17:2919–2932. 1989. View Article : Google Scholar : PubMed/NCBI
28	Esquela-Kerscher A and Slack FJ: Oncomirs-microRNAs with a role in cancer. Nat Rev Cancer. 6:259–269. 2006. View Article : Google Scholar : PubMed/NCBI
29	Bueno MJ and Malumbres M: MicroRNAs and the cell cycle. Biochim Biophys Acta. 1812:592–601. 2011. View Article : Google Scholar : PubMed/NCBI
30	Lieberman J, Slack F, Pandolfi PP, Chinnaiyan A, Agami R and Mendell JT: Noncoding RNAs and cancer. Cell. 153:9–10. 2013. View Article : Google Scholar : PubMed/NCBI
31	Kai-ming Wu, Yu-long He, Fa-keng Liu and Jian-hui Chen: Expression of mitochondrial transcription factor a in colon cancer and its role for proliferative regulation. Chinese Journal Of Bases And Clinics In General Surgery. 22:576–580. 2015.