Correlative analyses of the expression levels of PIAS3, p-SHP2, SOCS1 and SOCS3 with STAT3 activation in human astrocytomas

  • Authors:
    • Li‑Hong Liu
    • Hong Li
    • Xiao‑Xin Cheng
    • Qing‑You Kong
    • Xiao‑Yan Chen
    • Mo‑Li Wu
    • Yan Li
    • Jia Liu
    • Cong Li
  • View Affiliations

  • Published online on: December 28, 2016     https://doi.org/10.3892/mmr.2016.6079
  • Pages: 847-852
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Abstract

The importance of signal transducer and activator of transcription 3 (STAT3) signaling in the growth and survival of glioblastoma cells has been well documented, while the reasons leading to STAT3 activation remains to be elucidated. Suppressors of cytokine signaling (SOCS) 1 and SOCS3, SH2 domain‑containing phosphatase (SHP2) and protein inhibitors of activated STAT3 (PIAS3) are known to inhibit STAT3 signal transduction, while their expression statuses in the four grades of astrocytomas and relevance with STAT3 activation remain to be described. The present study aimed to address these issues by tissue microarray‑based immunohistochemical profiling the expression levels of phosphorylated (p)‑STAT3, SOCS1, SOCS3, PIAS3 and p‑SHP2. The results revealed that p‑STAT3 nuclear translocation was rarely observed in non‑cancerous brain tissues and its frequencies were increased in a tumor grade‑associated manner (65.2, 77.1, 81.8 and 85.7% for grade I‑IV, respectively). PIAS3, p‑SHP2, SOCS1 and SOCS3 were expressed in higher levels (++ and +++) in 63.6, 90, 87.5 and 81.8% of tumor surrounding brain tissues, which reduced to 13.1, 47.8, 33.3 and 50% in grade I, 11.4, 65.7, 58.3 and 77.1% in grade II, 9.1, 63.6, 38.1 and 31.8% in grade III and 7.1, 66.7, 30.8 and 7.1% in grade IV astrocytomas. The above results revealed that although the expression levels of SOCS1, SOCS3 and, in particular, p‑SHP2, tend to decrease in the four types of astrocytomas, PIAS3 downregulation is more negatively correlated with STAT3 activation in the stepwise progress of astrocytomas and would indicate an unfavorable outcome.
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February-2017
Volume 15 Issue 2

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Online ISSN:1791-3004

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Spandidos Publications style
Liu LH, Li H, Cheng XX, Kong QY, Chen XY, Wu ML, Li Y, Liu J and Li C: Correlative analyses of the expression levels of PIAS3, p-SHP2, SOCS1 and SOCS3 with STAT3 activation in human astrocytomas. Mol Med Rep 15: 847-852, 2017
APA
Liu, L., Li, H., Cheng, X., Kong, Q., Chen, X., Wu, M. ... Li, C. (2017). Correlative analyses of the expression levels of PIAS3, p-SHP2, SOCS1 and SOCS3 with STAT3 activation in human astrocytomas. Molecular Medicine Reports, 15, 847-852. https://doi.org/10.3892/mmr.2016.6079
MLA
Liu, L., Li, H., Cheng, X., Kong, Q., Chen, X., Wu, M., Li, Y., Liu, J., Li, C."Correlative analyses of the expression levels of PIAS3, p-SHP2, SOCS1 and SOCS3 with STAT3 activation in human astrocytomas". Molecular Medicine Reports 15.2 (2017): 847-852.
Chicago
Liu, L., Li, H., Cheng, X., Kong, Q., Chen, X., Wu, M., Li, Y., Liu, J., Li, C."Correlative analyses of the expression levels of PIAS3, p-SHP2, SOCS1 and SOCS3 with STAT3 activation in human astrocytomas". Molecular Medicine Reports 15, no. 2 (2017): 847-852. https://doi.org/10.3892/mmr.2016.6079