Effects of S100A6 gene silencing on the biological features of eutopic endometrial stromal cells and β‑catenin expression

Zhang,Xiaoling; Liu,Zequn; Chen,Meihong; Cao,Qing; Huang,Donghua

doi:10.3892/mmr.2017.6105

Effects of S100A6 gene silencing on the biological features of eutopic endometrial stromal cells and β‑catenin expression

Authors:
- Xiaoling Zhang
- Zequn Liu
- Meihong Chen
- Qing Cao
- Donghua Huang
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Affiliations: Department of Gynecology, Jiangxi Maternal and Child Health Hospital, Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Medicine, Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Jiangxi Province Key Laboratory of Molecular Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
Published online on: January 5, 2017 https://doi.org/10.3892/mmr.2017.6105
Pages: 1279-1285
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Protein expression levels of S100 calcium binding protein A6 (S100A6) are increased in various malignancies and are associated with tumor behavior; however, the association between S100A6 and endometriosis remains to be elucidated. In order to investigate the influence of S100A6 protein, recombinant lentivirus siS100A6 was used to transfect the eutopic endometrial stromal cells. CCK‑8 assay was performed to identify the proliferation ability of cell and the cell migration was detected by Transwell assay. Flow cytometry was performed to detect cell apoptosis, and western blotting and reverse transcription‑quantitative polymerase chain reaction were performed to identify the expression of β‑catenin. The present study investigated the role of S100A6 in endometriosis and its interaction with β‑catenin by transfecting eutopic endometrial stromal cells with a recombinant lentivirus containing S100A6‑specific small interfering RNA. Inhibition of S100A6 expression had a significant antiproliferative effect and reduced the migratory ability of eutopic endometrial stromal cells, and induced their apoptosis. In addition, inhibition of S100A6 expression suppressed β‑catenin expression. These results suggested that inhibition of S100A6 may represent a promising novel approach for the targeted therapy of endometriosis.

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