Effects of R type and S type ginsenoside Rg3 on DNA methylation in human hepatocarcinoma cells

Teng,Siying; Wang,Yi; Li,Pingya; Liu,Jinhua; Wei,Anhui; Wang,Haotian; Meng,Xiangkun; Pan,Di; Zhang,Xinmin

doi:10.3892/mmr.2017.6255

Effects of R type and S type ginsenoside Rg3 on DNA methylation in human hepatocarcinoma cells

Authors:
- Siying Teng
- Yi Wang
- Pingya Li
- Jinhua Liu
- Anhui Wei
- Haotian Wang
- Xiangkun Meng
- Di Pan
- Xinmin Zhang
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Affiliations: School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: February 28, 2017 https://doi.org/10.3892/mmr.2017.6255
Pages: 2029-2038
Copyright: © Teng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ginsenoside Rg3, a bioactive constituent isolated from Panax ginseng, exhibits antitumorigenic, antioxidative, antiangiogenic, neuroprotective and other biological activities are associated with the regulation of multiple genes. DNA methylation patterns, particularly those in the promoter region, affect gene expression, and DNA methylation is catalyzed by DNA methylases. However, whether ginsenoside Rg3 affects DNA methylation is unknown. High performance liquid chromatography assay, MspI/HpaII polymerase chain reaction (PCR) and reverse transcription‑quantitative PCR were performed to assess DNA methylation. It was demonstrated that 20(S)‑ginsenoside Rg3 treatment resulted in increased inhibition of cell growth, compared with treatment with 20(R)‑ginsenoside Rg3 in the human HepG2 hepatocarcinoma cell line. It was additionally revealed that treatment with 20(S)‑ginsenoside Rg3 reduced global genomic DNA methylation, altered cystosine methylation of the promoter regions of P53, B cell lymphoma 2 and vascular endothelial growth factor, and downregulated the expression of DNA methyltransferase (DNMT) 3a and DNMT3b more than treatment with 20(R)‑ginsenoside Rg3 in HepG2 cells. These results revealed that the modulation of DNA methylation may be important in the pharmaceutical activities of ginsenoside Rg3.

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