Age-associated alterations in constitutively expressed cyclooxygenase-2 immunoreactivity and protein levels in the hippocampus

Jung,Hyo  Young; Yoo,Dae  Young; Kim,Jong  Whi; Kwon,Hyun  Jung; Lee,Kwon  Young; Choi,Jung  Hoon; Kim,Dae  Won; Chung,Jin  Young; Yoon,Yeo  Sung; Hwang,In  Koo

doi:10.3892/mmr.2017.6512

Age-associated alterations in constitutively expressed cyclooxygenase-2 immunoreactivity and protein levels in the hippocampus

Authors:
- Hyo Young Jung
- Dae Young Yoo
- Jong Whi Kim
- Hyun Jung Kwon
- Kwon Young Lee
- Jung Hoon Choi
- Dae Won Kim
- Jin Young Chung
- Yeo Sung Yoon
- In Koo Hwang
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Affiliations: Department of Anatomy and Cell Biology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea, Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung‑Wonju National University, Gangneung, Gangwon 25457, Republic of Korea, Department of Anatomy, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Veterinary Internal Medicine and Geriatrics, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea
Published online on: April 26, 2017 https://doi.org/10.3892/mmr.2017.6512
Pages: 4333-4337

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Abstract

Cyclooxygenase-2 (COX-2) is a known inducible inflammatory mediator. COX-2 is constitutively expressed in the hippocampus and may regulate synaptic plasticity. The present study investigated the age‑associated alterations in white blood cell counts and hippocampal COX‑2 expression in healthy mice using immunohistochemical and western blot analyses at 1 month postnatal (PM1), PM3, PM6, PM12 and PM24. White blood cell counts were significantly decreased in the PM24 group when compared with the PM1 group. In addition, lymphocyte counts were decreased in the PM24 group when compared with all other groups. By contrast, monocyte, neutrophil and eosinophil counts were increased in the PM24 group; however, this did not reach statistical significance. COX‑2 expression was identified in the granule cells of the dentate gyrus and in the pyramidal cells of the hippocampal CA2/3 region. COX‑2 immunoreactivity was maintained until PM18, however, the levels significantly decreased by PM24. These results suggest that, despite alterations in the differential white blood cell counts, the significant decrease in constitutive COX‑2 expression in the hippocampus may be associated with degenerative age-associated alterations in synaptic plasticity in the hippocampus.

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