High‑throughput sequencing analyses of oral microbial diversity in healthy people and patients with dental caries and periodontal disease

Chen,Tingtao; Shi,Yan; Wang,Xiaolei; Wang,Xin; Meng,Fanjing; Yang,Shaoguo; Yang,Jian; Xin,Hongbo

doi:10.3892/mmr.2017.6593

High‑throughput sequencing analyses of oral microbial diversity in healthy people and patients with dental caries and periodontal disease

Authors:
- Tingtao Chen
- Yan Shi
- Xiaolei Wang
- Xin Wang
- Fanjing Meng
- Shaoguo Yang
- Jian Yang
- Hongbo Xin
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Affiliations: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, P.R. China, Department of Conservative Dentistry and Endodontics, Stomatological Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
Published online on: May 17, 2017 https://doi.org/10.3892/mmr.2017.6593
Pages: 127-132
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Recurrence of oral diseases caused by antibiotics has brought about an urgent requirement to explore the oral microbial diversity in the human oral cavity. In the present study, the high‑throughput sequencing method was adopted to compare the microbial diversity of healthy people and oral patients and sequence analysis was performed by UPARSE software package. The Venn results indicated that a mean of 315 operational taxonomic units (OTUs) was obtained, and 73, 64, 53, 19 and 18 common OTUs belonging to Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria and Fusobacteria, respectively, were identified in healthy people. Moreover, the reduction of Firmicutes and the increase of Proteobacteria in the children group, and the increase of Firmicutes and the reduction of Proteobacteria in the youth and adult groups, indicated that the age bracket and oral disease had largely influenced the tooth development and microbial development in the oral cavity. In addition, the traditional ‘pathogenic bacteria’ of Firmicutes, Proteobacteria and Bacteroidetes (accounted for >95% of the total sequencing number in each group) indicated that the ‘harmful’ bacteria may exert beneficial effects on oral health. Therefore, the data will provide certain clues for curing some oral diseases by the strategy of adjusting the disturbed microbial compositions in oral disease to healthy level.

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