Open Access

Bioinformatics analyses of the differences between lung adenocarcinoma and squamous cell carcinoma using The Cancer Genome Atlas expression data

  • Authors:
    • Fenghao Sun
    • Xiaodong Yang
    • Yulin Jin
    • Li Chen
    • Lin Wang
    • Mengkun Shi
    • Cheng Zhan
    • Yu Shi
    • Qun Wang
  • View Affiliations

  • Published online on: May 25, 2017     https://doi.org/10.3892/mmr.2017.6629
  • Pages: 609-616
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to explore gene and microRNA (miRNA) expression differences between lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). Differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) were identified by analyzing mRNA and miRNA expression data in normal and cancerous lung tissues that were obtained from The Cancer Genome Atlas database. A total of 778 DEGs and 7 DEMs were identified. Altered gene functions and signaling pathways were investigated using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses, which revealed that DEGs were significantly enriched in extracellular matrix organization, cell differentiation, negative regulation of toll signaling pathway, and several other terms and pathways. Transcription factor (TF)‑miRNA‑gene networks in LUAD and LUSC were predicted using the TargetScan, Miranda, and TRANSFAC databases, which revealed the regulatory links among the TFs, DEMs, and DEGs. The central TFs, i.e., the TFs in the middle of the TF‑miRNA‑gene network, of LUAD and LUSC were similar. Although LUAD and LUSC shared similar miRNAs in the predicted networks, miR‑29b‑3p was demonstrated to be upregulated only in LUAD, whereas miR‑1, miR‑105‑5p, and miR‑193b‑5p were altered in LUSC. These findings may improve our understanding of the different molecular mechanisms in non‑small cell lung cancers and may promote new and accurate strategies for prevention, diagnosis, and treatment.
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July-2017
Volume 16 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Sun F, Yang X, Jin Y, Chen L, Wang L, Shi M, Zhan C, Shi Y and Wang Q: Bioinformatics analyses of the differences between lung adenocarcinoma and squamous cell carcinoma using The Cancer Genome Atlas expression data. Mol Med Rep 16: 609-616, 2017
APA
Sun, F., Yang, X., Jin, Y., Chen, L., Wang, L., Shi, M. ... Wang, Q. (2017). Bioinformatics analyses of the differences between lung adenocarcinoma and squamous cell carcinoma using The Cancer Genome Atlas expression data. Molecular Medicine Reports, 16, 609-616. https://doi.org/10.3892/mmr.2017.6629
MLA
Sun, F., Yang, X., Jin, Y., Chen, L., Wang, L., Shi, M., Zhan, C., Shi, Y., Wang, Q."Bioinformatics analyses of the differences between lung adenocarcinoma and squamous cell carcinoma using The Cancer Genome Atlas expression data". Molecular Medicine Reports 16.1 (2017): 609-616.
Chicago
Sun, F., Yang, X., Jin, Y., Chen, L., Wang, L., Shi, M., Zhan, C., Shi, Y., Wang, Q."Bioinformatics analyses of the differences between lung adenocarcinoma and squamous cell carcinoma using The Cancer Genome Atlas expression data". Molecular Medicine Reports 16, no. 1 (2017): 609-616. https://doi.org/10.3892/mmr.2017.6629