Yi Guan Jian decoction may enhance hepatic differentiation of bone marrow‑derived mesenchymal stem cells via SDF‑1 in vitro

Fu,Lin‑Lin; Pang,Bing‑Yao; Zhu,Ying; Wang,Ling; Leng,Ai‑Jing; Chen,Hai‑Long

doi:10.3892/mmr.2017.6888

Yi Guan Jian decoction may enhance hepatic differentiation of bone marrow‑derived mesenchymal stem cells via SDF‑1 in vitro

Authors:
- Lin‑Lin Fu
- Bing‑Yao Pang
- Ying Zhu
- Ling Wang
- Ai‑Jing Leng
- Hai‑Long Chen
View Affiliations

Affiliations: Department of Infectious Disease, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Digestive Disease, Gansu Provincial Hospital, Lanzhou, Gansu 730000, P.R. China, Department of Chinese Medicine, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of General Surgery, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116011, P.R. China
Published online on: June 29, 2017 https://doi.org/10.3892/mmr.2017.6888
Pages: 2511-2521
Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

A previous study reported that Yi Guan Jian (YGJ) may increase the proliferation and differentiation of hepatic oval cells in a rat liver cirrhosis model. The aim of the present study was to investigate the effect and mechanism of action of YGJ on inducing hepatic differentiation in bone marrow‑derived mesenchymal stem cells (BM‑MSCs) via stromal‑cell derived factor‑1 (SDF‑1). Murine BM‑MSCs were isolated with whole bone marrow adherence, then identified by immunocytochemical staining and flow cytometry. Passage 2 cells were divided into 8 groups and their differentiation was induced by cell factors added to the medium, including hepatocyte growth factor (HGF), SDF‑1 and YGJ. Each of the cell factors was used alone and any two or three of them were combined to establish different cell microenvironments in the different treatment groups. Albumin (ALB) was selected as a hepatocellular marker and cytokeratin‑18 (CK‑18) as a cholangiocellular marker. The protein and mRNA expression levels of ALB and CK‑18 were used to determine the differentiation of BM‑MSCs using immunocytochemical staining, western blotting and reverse transcription‑quantitative polymerase chain reaction on days 7, 14, 21 and 28 during induction. The relative expression levels of ALB and CK‑18 resulted in time‑dependent increases in the groups supplemented only with HGF, SDF‑1 or YGJ. Combination treatment of any two HGF, SDF‑1 and YGJ led to a higher expression of ALB and CK‑18 compared with only one cell factor treatment. Additionally, when all three were used in a combined treatment the expression levels of ALB and CK‑18 occurred at an earlier time and was higher overall. Therefore, the present study suggested that YGJ had an effect on inducing hepatic differentiation in BM‑MSCs via SDF‑1 and may act in a synergistic manner with HGF and SDF‑1.

View Figures

View References

1	O'Grady J: Modern management of acute liver failure. Clin Liver Dis. 11:291–303. 2007. View Article : Google Scholar : PubMed/NCBI
2	Lei Y, Liu Z, Han Q, Kang W, Zhang L and Lou S: G-CSF enhanced SDF-1 gradient between bone marrow and liver associated with mobilization of peripheral blood CD34+ cells in rats with acute liver failure. Dig Dis Sci. 55:285–291. 2010. View Article : Google Scholar : PubMed/NCBI
3	Baertschiger RM, Serre-Beinier V, Morel P, Bosco D, Peyrou M, Clément S, Sgroi A, Kaelin A, Buhler LH and Gonelle-Gispert C: Fibrogenic potential of human multipotent mesenchymal stromal cells in injured liver. PLoS One. 4:66572009. View Article : Google Scholar
4	Amado LC, Saliaris AP, Schuleri KH, St John M, Xie JS, Cattaneo S, Durand DJ, Fitton T, Kuang JQ, Stewart G, et al: Cardiac repair with intramyocardial injection of allogeneic mesenchymal stem cells after myocardial infarction. Proc Natl AcadSci USA. 102:11474–11479. 2005. View Article : Google Scholar
5	Son BR, Marquez-Curtis LA, Kucia M, Wysoczynski M, Turner AR, Ratajczak J, Ratajczak MZ and Janowska-Wieczorek A: Migration of bone marrow and cord blood mesenchymal stem cells in vitro is regulated by stromal-derived factor-1-CXCR4 and hepatocyte growth factor-c-met axes and involves matrix metalloproteinases. Stem Cells. 24:1254–1264. 2006. View Article : Google Scholar : PubMed/NCBI
6	Askari AT, Unzek S, Popovic ZB, Goldman CK, Forudi F, Kiedrowski M, Rovner A, Ellis SG, Thomas JD, DiCorleto PE, et al: Effect of stromal-cell-derived factor 1 on stem-cell homing and tissue regeneration in ischaemic cardiomyopathy. Lancet. 362:697–703. 2003. View Article : Google Scholar : PubMed/NCBI
7	Lin HJ, Chen JY, Lin CF, Kao ST, Cheng JC, Chen HL and Chen CM: Hepatoprotective effects of Yi Guan Jian, an herbal medicine, in rats with dimethylnitrosamine-induced liver fibrosis. J Ethnopharmacol. 134:953–960. 2011. View Article : Google Scholar : PubMed/NCBI
8	Chen W, Chen JY, Tung YT, Chen HL, Kuo CW, Chuang CH, Chong KY, Mao FC and Chen CM: High-frequency ultrasound imaging to evaluate liver fibrosis progression in rats and Yi Guan Jian herbal therapeutic effects. Evid Based Complement Alternat Med. 2013:3023252013. View Article : Google Scholar : PubMed/NCBI
9	Wei ZX: Xu Ming Yi Lei An. 1770.(In Chinese).
10	Chen Z, Sun M, Yuan Q, Niu M, Yao C, Hou J, Wang H, Wen L, Liu Y, Li Z and He Z: Generation of functional hepatocytes from human spermatogonial stem cells. Oncotarget. 7:8879–8895. 2016. View Article : Google Scholar : PubMed/NCBI
11	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
12	Herzog EL, Chai L and Krause DS: Plasticity of marrow-derived stem cells. Blood. 102:3483–3493. 2003. View Article : Google Scholar : PubMed/NCBI
13	Jin SZ, Liu BR, Xu J, Gao FL, Hu ZJ, Wang XH, Pei FH, Hong Y, Hu HY and Han MZ: Ex vivo-expanded bone marrow stem cells home to the liver and ameliorate functional recovery in a mouse model of acute hepatic injury. Hepatobiliary Pancreat Dis Int. 11:66–73. 2012. View Article : Google Scholar : PubMed/NCBI
14	Ferrari G, Cusella-De Angelis G, Coletta M, Paolucci E, Stornaiuolo A, Cossu G and Mavilio F: Muscle regeneration by bone marrow-derived myogenic progenitors. Science. 279:1528–1530. 1998. View Article : Google Scholar : PubMed/NCBI
15	Hassink RJ, de la Brutel Rivière A, Mummery CL and Doevendans PA: Transplantation of cells for cardiac repair. J Am Coll Cardiol. 41:711–717. 2003. View Article : Google Scholar : PubMed/NCBI
16	Mezey E, Chandross KJ, Harta G, Maki RA and McKercher SR: Turning blood into brain: Cells bearing neuronal antigens generated in vivo from bone marrow. Science. 290:1779–1782. 2000. View Article : Google Scholar : PubMed/NCBI
17	Ayatollahi M, Soleimani M, Tabei SZ and Salmani M Kabir: Hepatogenic differentiation of mesenchymal stem cells induced by insulin like growth factor-I. World J Stem Cells. 3:113–121. 2011. View Article : Google Scholar : PubMed/NCBI
18	Petersen BE, Bowen WC, Patrene KD, Mars WM, Sullivan AK, Murase N, Boggs SS, Greenberger JS and Goff JP: Bone marrow as a potential source of hepatic ovel cells. Science. 284:1168–1170. 1990. View Article : Google Scholar
19	Theise ND, Badve S, Saxena R, Henegariu O, Sell S, Crawford JM and Krause DS: Derivitation of hepatocytes from bone marrow cells in mice after radiation-induced myeloablation. Hepatology. 31:235–240. 2003. View Article : Google Scholar
20	Hong H, Chen JZ, Zhou F, Xue L and Zhao GQ: Influence of serum from liver-damaged rats on differentiation tendency of bone marrow-derived stem cells. World J Gastroenterol. 10:2250–2253. 2004.PubMed/NCBI
21	Ohgushi H and Caplan AI: Stem cell technology and bioceramics: From cell to gene engineering. J Biomed Mater Res. 48:913–927. 1999. View Article : Google Scholar : PubMed/NCBI
22	Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI
23	Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR, Reyes M, Lenvik T, Lund T, Blackstad M, et al: Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. 418:41–49. 2002. View Article : Google Scholar : PubMed/NCBI
24	Polisetti N, Chaitanya VG, Babu PP and Vemuganti GK: Isolation, characterization and differentiation potential of rat bone marrow stromal cells. Neurol India. 58:201–208. 2010. View Article : Google Scholar : PubMed/NCBI
25	Friedenstein AJ, Chailakhyan RK and Gerasimov UV: Bone marrow osteogenic stem cell: In vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet. 20:263–272. 1987.PubMed/NCBI
26	Oh SH, Miyazaki M, Kouchi H, Inoue Y, Sakaguchi M, Tsuji T, Shima N, Higashio K and Namba M: Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro. Biochem Biophys Res Commun. 279:500–504. 2000. View Article : Google Scholar : PubMed/NCBI
27	Schwartz RE, Reyes M, Koodie L, Jiang Y, Blackstad M, Lund T, Lenvik T, Johnson S, Hu WS and Verfaillie CM: Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells. J Clin Invest. 109:1291–1302. 2002. View Article : Google Scholar : PubMed/NCBI
28	Lam SP, Luk JM, Man K, Ng KT, Cheung CK, Rose-John S and Lo CM: Activation of interleukin-6-induced glycoprotein 130/signal transducer and activator of transcription 3 pathway in mesenchymal stem cells enhances hepatic differentiation, proliferation, and liver regeneration. Liver Transpl. 16:1195–1206. 2010. View Article : Google Scholar : PubMed/NCBI
29	Martínez-Palacián A, del Castillo G, Herrera B, Fernández M, Roncero C, Fabregat I and Sánchez A: EGFR is dispensable for c-Met-mediated proliferation and survival activities in mouse adult liver oval cells. Cell Signal. 24:505–513. 2012. View Article : Google Scholar : PubMed/NCBI
30	Neuss S, Becher E, Wöltje M, Tietze L and Jahnen-Dechent W: Functional expression of HGF and HGF receptor/c-met in adult human mesenchymal stem cells suggests a role in cell mobilization, tissue repair, and wound healing. Stem Cells. 22:405–414. 2004. View Article : Google Scholar : PubMed/NCBI
31	Kaido T, Yamaoka S, Seto S, Funaki N, Kasamatsu T, Tanaka J, Nakamura T and Imamura M: Continuous hepatocyte growth factor supply prevents lipopolysaccharide-induced liver injury in rats. FEBS Lett. 411:378–382. 1997. View Article : Google Scholar : PubMed/NCBI
32	Xue F, Takahara T, Yata Y, Kuwabara Y, Shinno E, Nonome K, Minemura M, Takahara S, Li X, Yamato E and Watanabe A: Hepatocyte growth factor gene therapy accelerates regeneration in cirrhotic mouse livers after hepatectomy. Gut. 52:694–700. 2003. View Article : Google Scholar : PubMed/NCBI
33	Liu WL, You HJ and Che NC: The promotion of Yiguanjian on the recovery of liver fibrosis through mesenchymal stem cells: An experimental study. Global Traditional Chinese Med. 7:401–405. 2014.
34	Zhu Y and Liu P: Effect of Yi Guan Jian on proliferation and differentiation of hepatic oval cells in rat liver cirrhosis model set up by dimethylnitrosamine. J Dalian Med University. 33:11–16. 2011.(In Chinese).