Open Access

Identification of a gene expression profile associated with the regulation of angiogenesis in endometrial cancer

  • Authors:
    • Marcin Opławski
    • Mateusz Michalski
    • Andrzej Witek
    • Bogdan Michalski
    • Nikola Zmarzły
    • Agnieszka Jęda‑Golonka
    • Maria Styblińska
    • Joanna Gola
    • Małgorzata Kasprzyk‑Żyszczyńska
    • Urszula Mazurek
    • Andrzej Plewka
  • View Affiliations

  • Published online on: June 28, 2017     https://doi.org/10.3892/mmr.2017.6868
  • Pages: 2547-2555
  • Copyright: © Opławski et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The publication of the human genome sequence provided direction in the search for novel diagnostic and therapeutic methods for the treatment of human diseases. The aim of the present study was to investigate the hypothesis that the expression profile of genes involved in the regulation of angiogenesis may be a marker in endometrial cancer that facilitates the diagnosis and prognosis of patients, as well as the identification of novel therapeutic targets. The current study included 36 patients with grade (G) 1 to 3 endometrial cancer, and a control group of patients consisting of females that qualified for the removal of the uterus. Out of these, 28 samples (control, 3; G1, 7; G2, 12; and G3, 6) were selected for microarray analysis. Molecular analysis of the endometrial samples involved the extraction of total RNA, purification of the obtained extracts and subsequent analysis of the gene expression profiles using an oligonucleotide microarray technique (GeneChip® Human Genome U133A plates). The results indicated that the mRNA expression profile of genes involved in the regulation of angiogenesis varies depending on the degree of histological differentiation of endometrial adenocarcinoma. Similar results were obtained from descriptive statistics characterizing the expression profile of 691 mRNAs associated with the regulation of angiogenesis in the groups of patients with endometrial adenocarcinoma. In addition, the results of the present study indicated that neuropilin2 (NRP2) may serve an important role in the activity of endothelial cells, and may affect vascular endothelial growth factor, and potentially plexins and integrins via regulation of their functions. An understanding of how these proteins interact remains to be determined; however, elucidating these interactions may provide an explanation for the mechanisms underlying angiogenesis. In conclusion, the results of the present study suggest that NRP2 may be a valuable target for investigation in future pharmacological studies involving angiogenesis in endometrial cancer.
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September-2017
Volume 16 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Opławski M, Michalski M, Witek A, Michalski B, Zmarzły N, Jęda‑Golonka A, Styblińska M, Gola J, Kasprzyk‑Żyszczyńska M, Mazurek U, Mazurek U, et al: Identification of a gene expression profile associated with the regulation of angiogenesis in endometrial cancer. Mol Med Rep 16: 2547-2555, 2017
APA
Opławski, M., Michalski, M., Witek, A., Michalski, B., Zmarzły, N., Jęda‑Golonka, A. ... Plewka, A. (2017). Identification of a gene expression profile associated with the regulation of angiogenesis in endometrial cancer. Molecular Medicine Reports, 16, 2547-2555. https://doi.org/10.3892/mmr.2017.6868
MLA
Opławski, M., Michalski, M., Witek, A., Michalski, B., Zmarzły, N., Jęda‑Golonka, A., Styblińska, M., Gola, J., Kasprzyk‑Żyszczyńska, M., Mazurek, U., Plewka, A."Identification of a gene expression profile associated with the regulation of angiogenesis in endometrial cancer". Molecular Medicine Reports 16.3 (2017): 2547-2555.
Chicago
Opławski, M., Michalski, M., Witek, A., Michalski, B., Zmarzły, N., Jęda‑Golonka, A., Styblińska, M., Gola, J., Kasprzyk‑Żyszczyńska, M., Mazurek, U., Plewka, A."Identification of a gene expression profile associated with the regulation of angiogenesis in endometrial cancer". Molecular Medicine Reports 16, no. 3 (2017): 2547-2555. https://doi.org/10.3892/mmr.2017.6868