Evaluation of velvet antler total protein effect on bone marrow‑derived endothelial progenitor cells

Xiao,Xiang; Li,Lin; Xu,Shuqiang; Mao,Min; Pan,Ruiyan; Li,Yanjun; Wu,Jiayun; Huang,Li; Zheng,Xiaoyun

doi:10.3892/mmr.2017.7019

Evaluation of velvet antler total protein effect on bone marrow‑derived endothelial progenitor cells

Authors:
- Xiang Xiao
- Lin Li
- Shuqiang Xu
- Min Mao
- Ruiyan Pan
- Yanjun Li
- Jiayun Wu
- Li Huang
- Xiaoyun Zheng
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Affiliations: Graduate School, Beijing University of Chinese Medicine, Beijing 100029, P.R. China, National Integrated Traditional and Western Medicine Center for Cardiovascular Disease, China‑Japan Friendship Hospital, Beijing 100029, P.R. China, Emergency Office, National Health and Family Planning Commission of the People's Republic of China, Beijing 100044, P.R. China, Pharmaceutical Department, China‑Japan Friendship Hospital, Beijing 100029, P.R. China, Department of Pharmacology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, P.R. China, Department of Senior Official Ward, China‑Japan Friendship Hospital, Beijing 100029, P.R. China
Published online on: July 15, 2017 https://doi.org/10.3892/mmr.2017.7019
Pages: 3161-3168
Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lu Rong, velvet antler (VA), is a traditional Chinese medicine, which is used as a food supplement and therapeutic drug in China, Japan, Russia, New Zealand and Southeast Asia. The regenerative characteristics of VA have resulted in great research interest, particularly regarding the fields of organ grafting and stem cell differentiation. Various VA proteomic studies verified that proteins act as the primary bioactive components of VA. The present study aimed to investigate if VA proteins (VA‑pro) influence endothelial progenitor cell (EPC) viability. Various methods have previously been used to investigate VA‑pro, including freeze‑drying technology, ultrasonic wave methods, high performance liquid chromatography‑mass spectrometry, EPCs extraction and culture. Results demonstrated that VA‑pro promoted EPCs proliferation and migration, particularly at a concentration of 1 mg/ml. Furthermore, VA‑pro increased the activation level of Notch1 intracellular domain and Hes1, and the level of phosphorylated‑Akt and phosphorylated‑mechanistic target of rapamycin. VA‑pro may therefore affect EPC viability via regulation of the Notch and Akt signaling pathways. The present study revealed the effects and potential molecular mechanism of VA‑pro on EPCs, and suggested an association between VA regeneration characteristics and the optimization of EPC viability. These findings may contribute to EPC transplantation research and aid in providing a novel treatment method for vascular diseases in the future.

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