Chronic administration of SUMO‑1 has negative effects on novel object recognition memory as well as cell proliferation and neuroblast differentiation in the mouse dentate gyrus

Yoo,Dae Young; Kim,Dae Won; Kwon,Hyun Jung; Jung,Hyo Young; Nam,Sung Min; Kim,Jong Whi; Chung,Jin Young; Won,Moo‑Ho; Yoon,Yeo Sung; Choi,Soo Young; Hwang,In Koo

doi:10.3892/mmr.2017.6946

Chronic administration of SUMO‑1 has negative effects on novel object recognition memory as well as cell proliferation and neuroblast differentiation in the mouse dentate gyrus

Authors:
- Dae Young Yoo
- Dae Won Kim
- Hyun Jung Kwon
- Hyo Young Jung
- Sung Min Nam
- Jong Whi Kim
- Jin Young Chung
- Moo‑Ho Won
- Yeo Sung Yoon
- Soo Young Choi
- In Koo Hwang
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Affiliations: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea, Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Kangneung‑Wonju National University, Gangneung 25457, Republic of Korea, Department of Veterinary Internal Medicine and Geriatrics, College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea, Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea, Department of Biomedical Sciences, Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea
Published online on: July 12, 2017 https://doi.org/10.3892/mmr.2017.6946
Pages: 3427-3432

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Abstract

Post‑translational modifications have been associated with developmental and aging processes, as well as in the pathogenesis of certain diseases. The present study aimed to investigate the effects of small ubiquitin‑like modifier 1 (SUMO‑1) on hippocampal dependent memory function, cell proliferation and neuroblast differentiation. To facilitate the delivery of SUMO‑1 into hippocampal neurons, a transactivator of transcription (Tat)‑SUMO‑1 fusion protein was constructed and mice were divided into two groups: A vehicle (Tat peptide)‑treated group and a Tat‑SUMO‑1‑treated group. The vehicle or Tat‑SUMO‑1 was administered intraperitoneally to 7‑week‑old mice once daily for 3 weeks, and a novel object recognition test was conducted following the final treatment; the animals were sacrificed 2 h following the test for further analysis. Administration of Tat‑SUMO‑1 significantly decreased exploration of a new object in a novel object recognition test compared with mice in the vehicle‑treated group. In addition, cell proliferation and neuroblast differentiation analyses (based on Ki67 and doublecortin immunohistochemistry, respectively) revealed that the administration of Tat‑SUMO‑1 significantly reduced cell proliferation and neuroblast differentiation in the dentate gyrus. These results suggested that chronic supplementation of Tat‑SUMO‑1 affects hippocampal functions by decreasing cell proliferation and neuroblast differentiation in the mouse dentate gyrus.

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