Suppression of TAFI by siRNA inhibits invasion and migration of breast cancer cells

  • Authors:
    • Chao Yu
    • Yun Luan
    • Zejun Wang
    • Jingjie Zhao
    • Chengwei Xu
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  • Published online on: July 18, 2017     https://doi.org/10.3892/mmr.2017.7031
  • Pages: 3469-3474
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Abstract

Human thrombin activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2), is a procarboxypeptidase enzyme. The purpose of the present study was to observe the expression of TAFI in breast cancer (BC) and breast cancer cell (BCC) lines and to investigate the effect of TAFI suppression by small interfering (si)RNA gene silencing on invasion and migration of BCC lines. A significant increase in TAFI level was identified by immunohistochemical analysis in BC tissues compared with normal breast tissues. TAFI suppression also inhibited cell viability, invasion and migration ability as demonstrated by MTT, Transwell chamber, and wound scratch assays, respectively (P<0.05). The data suggested that suppression of TAFI by siRNA inhibits invasion and migration of breast cancer cells and that TAFI may be a new target for breast cancer therapy.
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September-2017
Volume 16 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yu C, Luan Y, Wang Z, Zhao J and Xu C: Suppression of TAFI by siRNA inhibits invasion and migration of breast cancer cells. Mol Med Rep 16: 3469-3474, 2017
APA
Yu, C., Luan, Y., Wang, Z., Zhao, J., & Xu, C. (2017). Suppression of TAFI by siRNA inhibits invasion and migration of breast cancer cells. Molecular Medicine Reports, 16, 3469-3474. https://doi.org/10.3892/mmr.2017.7031
MLA
Yu, C., Luan, Y., Wang, Z., Zhao, J., Xu, C."Suppression of TAFI by siRNA inhibits invasion and migration of breast cancer cells". Molecular Medicine Reports 16.3 (2017): 3469-3474.
Chicago
Yu, C., Luan, Y., Wang, Z., Zhao, J., Xu, C."Suppression of TAFI by siRNA inhibits invasion and migration of breast cancer cells". Molecular Medicine Reports 16, no. 3 (2017): 3469-3474. https://doi.org/10.3892/mmr.2017.7031