Dynamic expression analysis of armc10, the homologous gene of human GPRASP2, in zebrafish embryos

Liu,Chunyu; Lin,Changsong; Yao,Jun; Wei,Qinjun; Xing,Guangqian; Cao,Xin

doi:10.3892/mmr.2017.7357

Dynamic expression analysis of armc10, the homologous gene of human GPRASP2, in zebrafish embryos

Authors:
- Chunyu Liu
- Changsong Lin
- Jun Yao
- Qinjun Wei
- Guangqian Xing
- Xin Cao
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Affiliations: Department of Biotechnology, School of Basic Medicinal Sciences, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China, Department of Otolaryngology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
Published online on: August 24, 2017 https://doi.org/10.3892/mmr.2017.7357
Pages: 5931-5937
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

G protein‑coupled receptor‑associated sorting protein 2 (GPRASP2), a member of the GASP family, has been reported to be involved in the modulation of transcription. However, few studies have revealed the role of GPRASP2 in the development and progression of diseases. As a model organism, zebrafish have been widely used to investigate human diseases. In the present study, zebrafish armadillo repeat‑containing 10 (armc10), an orthologous gene of human GPRASP2 was identified, and the spatial and temporal expression patterns of armc10 in zebrafish during early embryonic development were revealed. Bioinformatics analyses showed that ARMC10 protein sequences were highly conserved. Reverse transcription polymerase chain reaction analysis and whole mount in situ hybridization revealed that zebrafish armc10 was maternally expressed and was detected at a weak level up to 12 h post‑fertilization (hpf), however, its expression increased to a high level at 24 hpf. At the 75% epiboly stage and 12 hpf, armc10 was widely expressed in the embryo. At 24 hpf, armc10 mRNA was expressed in the nervous system of the zebrafish head. When the embryo was 2 days old, the wide expression of armc10 was maintained in the nervous system of the zebrafish head. At 72 hpf, the mRNA expression of armc10 was located specifically in the otic vesicles in addition to the nervous system of the head. At 96 hpf, the expression of armc10 was maintained in the otic vesicles and the nervous system of the head. The results of the present study provided novel insight into the spatial and temporal mRNA expression of armc10 in zebrafish, for the further investigation of nervous system diseases.

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