Exposure of keratinocytes to non‑thermal dielectric barrier discharge plasma increases the level of 8‑oxoguanine via inhibition of its repair enzyme

  • Authors:
    • Ki Cheon Kim
    • Madduma Hewage Susara Ruwan Kumara
    • Kyoung Ah Kang
    • Mei Jing Piao
    • Min Chang Oh
    • Yea Seong Ryu
    • Jin Oh Jo
    • Young Sun Mok
    • Jennifer H. Shin
    • Yeunsoo Park
    • Seong Bong Kim
    • Suk Jae Yoo
    • Jin Won Hyun
  • View Affiliations

  • Published online on: September 11, 2017     https://doi.org/10.3892/mmr.2017.7454
  • Pages: 6870-6875
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Abstract

Oxidative stress enhances cellular DNA oxidation and may cause mutations in DNA bases, including 8‑oxoguanine (8‑oxoG). Our recent study reported that exposure of cells to non‑thermal dielectric barrier discharge (DBD) plasma generates reactive oxygen species and damages DNA. The present study investigated the effect of non‑thermal DBD plasma exposure on the formation of 8‑oxoG in HaCaT human keratinocytes. Cells exposed to DBD plasma exhibited increased level of 8‑oxoG. In addition, mRNA and protein expression levels of 8‑oxoguanine glycosylase 1 (OGG1), an 8‑oxoG repair enzyme, were reduced in plasma‑exposed cells. Furthermore, the expression level of nuclear factor erythroid 2‑related factor 2 (Nrf2), a transcription factor that regulates OGG1 gene expression, was reduced following exposure to DBD plasma. Pretreatment of cells with an antioxidant, N‑acetyl cysteine (NAC), prior to plasma exposure suppressed the formation of 8‑oxoG and restored the expression levels of OGG1 and Nrf2. In addition, phosphorylation of protein kinase B (Akt), which regulates the activation of Nrf2, was reduced following plasma exposure. However, phosphorylation was restored by pretreatment with NAC. These findings suggested that non‑thermal DBD plasma exposure generates 8‑oxoG via inhibition of the Akt‑Nrf2‑OGG1 signaling pathway in HaCaT cells.
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November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kim K, Ruwan Kumara M, Kang K, Piao M, Oh M, Ryu Y, Jo J, Mok Y, Shin JH, Park Y, Park Y, et al: Exposure of keratinocytes to non‑thermal dielectric barrier discharge plasma increases the level of 8‑oxoguanine via inhibition of its repair enzyme. Mol Med Rep 16: 6870-6875, 2017.
APA
Kim, K., Ruwan Kumara, M., Kang, K., Piao, M., Oh, M., Ryu, Y. ... Hyun, J. (2017). Exposure of keratinocytes to non‑thermal dielectric barrier discharge plasma increases the level of 8‑oxoguanine via inhibition of its repair enzyme. Molecular Medicine Reports, 16, 6870-6875. https://doi.org/10.3892/mmr.2017.7454
MLA
Kim, K., Ruwan Kumara, M., Kang, K., Piao, M., Oh, M., Ryu, Y., Jo, J., Mok, Y., Shin, J. H., Park, Y., Kim, S., Yoo, S., Hyun, J."Exposure of keratinocytes to non‑thermal dielectric barrier discharge plasma increases the level of 8‑oxoguanine via inhibition of its repair enzyme". Molecular Medicine Reports 16.5 (2017): 6870-6875.
Chicago
Kim, K., Ruwan Kumara, M., Kang, K., Piao, M., Oh, M., Ryu, Y., Jo, J., Mok, Y., Shin, J. H., Park, Y., Kim, S., Yoo, S., Hyun, J."Exposure of keratinocytes to non‑thermal dielectric barrier discharge plasma increases the level of 8‑oxoguanine via inhibition of its repair enzyme". Molecular Medicine Reports 16, no. 5 (2017): 6870-6875. https://doi.org/10.3892/mmr.2017.7454